Vascular Endothelial Glycocalyx and Interstitial Fluid Excretion
| Project/Area Number |
19H03756
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 55060:Emergency medicine-related
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| Research Institution | Gifu University |
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Principal Investigator |
Okada Hideshi 岐阜大学, 大学院医学系研究科, 准教授 (30402176)
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| Co-Investigator(Kenkyū-buntansha) |
渡邉 崇量 岐阜大学, 医学部附属病院, 助教 (30509435)
富田 弘之 岐阜大学, 大学院医学系研究科, 准教授 (50509510)
宮崎 渚 朝日大学, 歯学部, 准教授 (50550141)
竹村 元三 朝日大学, 歯学部, 教授 (40283311)
鈴木 浩大 岐阜大学, 医学部附属病院, 助教 (80724583)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2020: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2019: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 血管内皮グリコカリックス / 血管透過性 / 体液貯留 / 心不全 / 血管炎 / 血管内皮障害 / グリコカリックス / 腎不全 / 血管内皮 / 微小循環 |
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| Outline of Research at the Start |
正常時に血管内に存在するグリコカリックスは炎症により障害され、血管透過性亢進が生じる。この中で浮腫により開大した血管外腔の周辺組織表面を覆うように薄いグリコカリックス層が出現することを確認した。この現象は、グリコカリックスが血管外に漏出し、血管外腔周辺組織にグリコカリックスが付着した状態と考えられ、間質に漏れ出した水分がグリコカリックスと血管外組織により構成される水路を通ってリンパ管へ排出される代償機構であると仮定した。本研究では、この血管外に生じたグリコカリックスの由来と役割を明らかにし、浮腫改善による臓器障害抑制を目的とした治療を開発する。
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| Outline of Final Research Achievements |
Glycocalyx presents on vascular endothelium under normal conditions is injured by inflammation. We observed a thin layer of glycocalyx covering the surface of the tissue surrounding the open vessel lumen due to edema, and investigated whether this layer was derived from the injured endothelial glycocalyx. Using a mouse model of heart failure showing fluid retention, structures depicted by lanthanum nitrate were identified in the perivascular tissue. Furthermore, when thrombomodulin was administered to a lipopolysaccharide-induced vasculitis model, the efflux of lanthanum nitrate out of blood vessels was suppressed. Although it is still unclear whether eGCX migrates into the perivascular lumen, lanthanum nitrate may be an indicator of vascular permeability.
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| Academic Significance and Societal Importance of the Research Achievements |
グリコカリックスは血管の恒常性維持に重要な役割を果たしており、その機能維持・回復は新しい治療の開発のターゲットとして注目されている。本研究は障害されて剥離したグリコカリックスと浮腫の関連に注目した極めて独創的な研究である。臨床においては血管透過性を表すマーカーについては未だ確立したものがないが、今回の研究において、硝酸ランタンを臨床においても散ることはできないものの、血管透過性のマーカーとして血管内の構造物がターゲットとなり得る可能性を示唆した点において社会的意義があったと考える。
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Severe heat stroke complicated by multiple cerebral infarctions: a case report2021
Author(s)
Kamidani Ryo、Okada Hideshi、Kitagawa Yuichiro、Kusuzawa Keigo、Ichihashi Masahiro、Kakino Yoshinori、Oiwa Hideaki、Yasuda Ryu、Fukuta Tetsuya、Yoshiyama Naomasa、Miyake Takahito、Okamoto Haruka、Suzuki Kodai、Yamada Noriaki、Doi Tomoaki、Yoshida Takahiro、Ushikoshi Hiroaki、Kumada Keisuke、Yoshida Shozo、Ogura Shinji
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Journal Title
Journal of Medical Case Reports
Volume: 15
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Glucose transporter Glut1 controls diffuse invasion phenotype with perineuronal satellitosis in diffuse glioma microenvironment.2020
Author(s)
Miyai M, Kanayama T, Hyodo F, Kinoshita T, Ishihara T, Okada H, Suzuki H, Takashima S, Wu Z, Hatano Y, Egashira Y, Enomoto Y, Nakayama N, Soeda A, Yano H, Hirata A, Niwa M, Sugie S, Mori T, Maekawa Y, Iwama T, Matsuo M, Hara A, Tomita H
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Journal Title
Neurooncol Adv.
Volume: 30
Issue: 1
Pages: 06198-06198
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Vacuolated cardiomyocytes in human endomyocardial biopsy specimens2020
Author(s)
Takemura Genzou、Onoue Kenji、Arimoto Takanori、Watanabe Tetsu、Tsujimoto Akiko、Takada Chihiro、Okada Hideshi、Nakano Tomoya、Sakaguchi Yasuhiro、Miyazaki Nagisa、Watanabe Takatomo、Kanamori Hiromitsu、Ogura Shinji、Saito Yoshihiko、Fujiwara Takako、Fujiwara Hisayoshi、Hotta Yasuaki
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Journal Title
Journal of Cardiology Cases
Volume: 21
Issue: 2
Pages: 54-58
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Diffuse midline glioma in the spinal cord with rapid respiratory deterioration: A case report2020
Author(s)
Kamidani R, Okada H, Yasuda R, Yoshida T, Kasuzawa K, Ichihasi M, Kakino Y, Oiwa H, Kitagawa Y, Fukuta T, Suzuki K, Okamoto H , Miyake T, Tachi M, Kanada N, Iwai C, Shiba M, Yamada N, Nozawa S, Nakayama N, Doi T, Fushimi K, Yano H, Tomita H, Yoshida S, Iwama T, Ogura S.
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Journal Title
Acute Medicine & Surgery
Volume: 2020
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Neutrophil Elastase Inhibition Ameliorates Endotoxin-Induced Myocardial Injury Accompanying Degradation of Cardiac Capillary Glycocalyx2019
Author(s)
Fukuta T, Okada H, Takemura G, Suzuki K, Takada C, Tomita H, Suzuki A, Oda K, Uchida A, Matsuo S, Fukuda H, Yano H, Muraki I, Zaikokuji R, Kuroda A, Nishio A, Sampei S, Miyazaki N, Hotta Y, Yamada N, Watanabe T, Morishita K, Doi T, Yoshida T, Ushikoshi H, Yoshida S, Maekawa Y, Ogura S
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Journal Title
Shock
Volume: 2019 Nov 19.
Issue: 3
Pages: 1-1
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Factors Enhancing Serum Syndecan-1 Concentrations: A Large-Scale Comprehensive Medical Examination2019
Author(s)
Oda K, Okada H, Suzuki A, Tomita H, Kobayashi R, Sumi K, Suzuki K, Takada C, Ishihara T, Suzuki K, Kano S, Kondo K, Iwashita Y, Yano H, Zaikokuji R, Sampei S, Fukuta T, Kitagawa Y, Okamoto H, Watanabe T, Kawaguchi T, Kojima T, Deguchi F, Miyazaki N, Yamada N, Doi T, Yoshida T et al.
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Journal Title
Journal of Clinical Medicine
Volume: 8
Issue: 9
Pages: 1320-1320
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Human Colorectal Cancer Infrastructure Constructed by the Glycocalyx.2019
Author(s)
Tachi M, Okada H, Matsuhashi N, Takemura G, Suzuki K, Fukuda H, Niwa A, Tanaka T, Mori H, Hara A, Yoshida K, Ogura S, Tomita H
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Journal Title
J Clin Med
Volume: 8(9)
Issue: 9
Pages: 1270-1270
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia2019
Author(s)
Suzuki K, Okada H, Takemura G, Takada C, Kuroda A, Yano H, Zaikokuji R, Morishita K, Tomita H, Oda K, Matsuo S, Uchida A, Fukuta T, Sampei S, Miyazaki N, Kawaguchi T, Watanabe T, Yoshida T, Ushikoshi H, Yoshida S, Maekawa Y, Ogura S.
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Journal Title
The American Journal of Pathology
Volume: 189
Issue: 8
Pages: 1526-1535
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] High concentration oxygen administration injures pulmonary microcirculation2020
Author(s)
Ryo Kamidani, Hideshi Okada, Genzou Takemura, Chihiro Takada, Kodai Suzuki, Hirohisa Yano, Ayumi Kuroda, Tetsuya Fukuta, Takahito Miyake, Noriaki Yamada Norihide hide Kanda, Hiroaki Ushikoshi, Shozo Yoshida, Shinji Ogura
Organizer
American Thoracic Society 2020
Related Report
Int'l Joint Research
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[Presentation] Recombinant Thrombomodulin Protect Cardiac Capillary Endothelial Glycocalyx under Experimental Endotoxemia2020
Author(s)
Yuichiro Kitagawa, Hideshi Okada, Genzou Takemura, Kodai Suzuki, Chihiro Takada, Hiroyuki Tomita, Shigeo Takashima, Tomoaki Doi, Takahiro Yoshida, Hiroaki Ushikoshi, Shozo Yoshida, Shinji Ogura
Organizer
American Thoracic Society 2020
Related Report
Int'l Joint Research
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