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Development of Bone Organoid Models Towards the Precision Prosthodontics

Research Project

Project/Area Number 19H03840
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 57050:Prosthodontics-related
Research InstitutionTohoku University

Principal Investigator

Egusa Hiroshi  東北大学, 歯学研究科, 教授 (30379078)

Co-Investigator(Kenkyū-buntansha) 新部 邦透  東北大学, 大学病院, 講師 (50468500)
山田 将博  東北大学, 歯学研究科, 准教授 (90549982)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2020: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
KeywordsiPS細胞 / オルガノイド / 骨 / 軟骨 / 歯胚 / 自己組織化 / 生体機能チップ / 歯科補綴学 / 骨機能チップ
Outline of Research at the Start

歯を失った後の顎堤吸収や骨再生の「個人差」は補綴・インプラント治療に大きな影響を与える。本研究では,生体組織を疑似した骨オルガノイド培養系を用いることで,患者個人の骨代謝・骨再生を試験管内に再現し,術前診断に活用する技術基盤の構築を目的とする。そのために,患者の体質を反映するiPS細胞を用いた骨の生体機能チップ(Organ-on-a-chip)開発を試み,“Precision Prosthodontics(高精度補綴歯科治療)”への道筋を探索する。

Outline of Final Research Achievements

Recently, organoid-on-a-chip technologies, which enable us to reproduce biological function of cells and tissues at the organ level in vitro, commands considerable attention. The aim of this study was to establish culture methods for the bone/cartilage/tooth organoids-on-a-chip using induced pluripotent stem (iPS) cell technology towards creating precision prosthodontics. We established induction method of iPS cells to bone organoid using micro-space culture wells. We also found a method to induce the bone/cartilage hybrid by applying a shaking culture. Furthermore, we established a step-wise culture method to induce iPS cells to enamelblasts. These results would contribute to the production of bone/cartilage/tooth organoids-on-a-chip in the future, and may lead to the basic technology of precision prosthetics that reproduces patient hard tissues in vitro.

Academic Significance and Societal Importance of the Research Achievements

近年、個人差を適切な治療法の選択に利用する精密医療の有用性が認識されており、個人差の術前診断に向けたヒトの病態・体質を試験管内で再現・評価するモデル(オルガノイド)の開発が重要となっている。本研究は、万能細胞として知られるiPS細胞を利用して、試験管内で骨、軟骨、歯のオルガノイドの作製技術を検討したものであり、その成果は顎の骨や歯の個人差を分子生物学的に検出する基盤技術に繋がることが期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (25 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (6 results) Journal Article (7 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (11 results) (of which Int'l Joint Research: 6 results,  Invited: 11 results) Book (1 results)

  • [Int'l Joint Research] Chulalongkorn University(タイ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Shanghai Jiao Tong University(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Chulalongkorn University(タイ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Shanghai Jiao Tong University(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Chulalongkorn University(タイ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Shanghai Jiao Tong University(中国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Intermittent compressive force induces cell cycling and reduces apoptosis in embryoid bodies of mouse induced pluripotent stem cells2022

    • Author(s)
      Manokawinchoke J, Limraksasin P, Okawa H, Pavasant P, Egusa H, Osathanon T
    • Journal Title

      Int J Oral Sci

      Volume: 14 Issue: 1 Pages: 1-1

    • DOI

      10.1038/s41368-021-00151-3

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Stage-Specific Role of Amelx Activation in Stepwise Ameloblast Induction from Mouse Induced Pluripotent Stem Cells2021

    • Author(s)
      Miao Xinchao、Niibe Kunimichi、Zhang Maolin、Liu Zeni、Nattasit Praphawi、Ohori-Morita Yumi、Nakamura Takashi、Jiang Xinquan、Egusa Hiroshi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 13 Pages: 7195-7195

    • DOI

      10.3390/ijms22137195

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mechanical loading and the control of stem cell behavior2021

    • Author(s)
      Manokawinchoke J, Pavasant P, Limjeerajarus CN, Limjeerajarus N, Osathanon T, Egusa H
    • Journal Title

      Arch Oral Biol

      Volume: 125 Pages: 105092-105092

    • DOI

      10.1016/j.archoralbio.2021.105092

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A method for in vitro fabrication of hybrid bone/cartilage tissue using mouse induced pluripotent stem cells2021

    • Author(s)
      Limraksasin P, Egusa H
    • Journal Title

      Methods Mol Biol

      Volume: - Pages: 509-520

    • DOI

      10.1007/7651_2021_361

    • ISBN
      9781071621189, 9781071621196
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Shaking culture enhances chondrogenic differentiation of mouse induced pluripotent stem cell constructs2020

    • Author(s)
      Limraksasin P, Kosaka Y, Zhang M, Horie N, Kondo T, Okawa H, Yamada M, Egusa H
    • Journal Title

      Sci Rep

      Volume: 10 Issue: 1 Pages: 14996-14996

    • DOI

      10.1038/s41598-020-72038-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In vitro fabrication of hybrid bone/cartilage complex using mouse induced pluripotent stem cells2020

    • Author(s)
      Limraksasin P, Kondo T, Zhang M, Okawa H, Osathanon T, Pavasant P, Egusa H
    • Journal Title

      Int J Mol Sci

      Volume: 21 Issue: 2 Pages: 581-581

    • DOI

      10.3390/ijms21020581

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Size-optimized micro-space culture facilitates differentiation of mouse induced pluripotent stem cells into osteoid-rich bone constructs2020

    • Author(s)
      Limraksasin P, Okawa H, Zhang M, Kondo T, Osathanon T, Pavasant P, Egusa H
    • Journal Title

      Stem Cells Int

      Volume: 2020 Pages: 7082679-7082679

    • DOI

      10.1155/2020/7082679

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 細胞を利用した再生歯科補綴治療法の開発2022

    • Author(s)
      江草 宏
    • Organizer
      第21回 再生医療学会総会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] iPS cell-based strategies in bone tissue engineering2021

    • Author(s)
      Hiroshi Egusa
    • Organizer
      Society of Dental Material Science, Chinese Stomatological Association & Academy of Dental Materials Joint Hybrid Meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Biodental Engineering ~2040 年の補綴歯科治療に向けて~2021

    • Author(s)
      江草 宏
    • Organizer
      第24回 日本歯科医学会学術大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 次世代の再生補綴歯科治療に向けたイノベーションロードマップ2021

    • Author(s)
      江草 宏
    • Organizer
      第63回歯科基礎医学会学術大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 細胞を利用した再生歯科補綴治療法の開発2021

    • Author(s)
      江草 宏
    • Organizer
      第19回 日本再生歯科医学会学術大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Emerging Approaches for Regenerative Prosthodontics2020

    • Author(s)
      Hiroshi Egusa
    • Organizer
      1st Chiang Mai University-Tohoku University Special Online Dental Meeting 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Emerging Approaches for Regenerative Prosthodontics2020

    • Author(s)
      Hiroshi Egusa
    • Organizer
      8th International Students’ Dental Comference 2020, University of Sharjah Dental Student Association
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] iPS細胞を用いた骨/軟骨バイオエンジニアリング2019

    • Author(s)
      江草 宏
    • Organizer
      第128回 日本補綴歯科学会学術大会 シンポジウム「Biodental Engineering-再生歯科補綴に向けた人工臓器の創成-」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Emerging Approaches for Regenerative Prosthodontics2019

    • Author(s)
      Hiroshi Egusa
    • Organizer
      18th International College of Prosthodontists & 43rd European Prosthodontic Association
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] iPS Cell-Based Strategies in Bone Tissue Engineering2019

    • Author(s)
      Hiroshi Egusa
    • Organizer
      2nd Joint Symposium Between School of Stomatology Wuhan University and Tohoku University Graduate School of Dentistry
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Emerging Approaches for Regenerative Prosthodontics2019

    • Author(s)
      Hiroshi Egusa
    • Organizer
      Tohoku-Taiwan-YangMing Dental Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] 関節・軟骨の再生医療(執筆担当:軟骨内骨化を利用した顎骨再生のアプローチ)2019

    • Author(s)
      堀江尚弘,近藤 威,江草 宏
    • Total Pages
      250
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314860
    • Related Report
      2019 Annual Research Report

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Published: 2019-04-18   Modified: 2023-01-30  

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