| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2023: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2022: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2021: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Outline of Final Research Achievements |
We developed the world's widest-field and fastest two-photon microscope (FASHIO-2PM) (Ota et al. Neuron 2021). In this research project, Aonishi was contributing in developing a low-computational-cost cell detection algorithm, LCCD, which automatically detects cells at high speed from the large-scale imaging data acquired by FASHIO-2PM. We succeeded in automatically detecting the activity of 16,000 cells from 15 brain regions (3mm x 3mm) including sensory, motor, and higher-level areas with high accuracy and high speed (Ito et al. Neurosci. Res. 2022). To improve cell detection performance, the fluorescent signal corresponding to the neuropil signal was detected and deleted from the original signal.
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