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In-situ determination of denitrification, assimilation, and nitrification rates in streams using triple oxygen isotopes of dissolved nitrate as tracer

Research Project

Project/Area Number 19H04254
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 63010:Environmental dynamic analysis-related
Research InstitutionNagoya University

Principal Investigator

Nakagawa Fumiko  名古屋大学, 環境学研究科, 准教授 (70360899)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Keywords河川 / 脱窒速度 / 硝化速度 / 同化速度 / 三酸素同位体組成 / 大気硝酸濃度 / 硝酸 / 三酸素同位体異常 / 大気硝酸 / 窒素循環 / 脱窒・硝化・同化速度 / 河川環境 / 脱窒 / 同化
Outline of Research at the Start

本研究では、河川水に溶存する硝酸の濃度と三酸素同位体組成から算出される大気硝酸濃度に着目し、この時空間変化を追跡することによって、河川環境下における脱窒速度を中心とした窒素循環速度を見積もる新手法を開発し、検証する。この新手法は天然トレーサーをそのまま使っており、人工トレーサーを添加したり培養したりすることなく自然の状態のまま河床を含めた河川環境全体の脱窒速度を定量できる画期的手法であり、あらゆる河川環境で利用できるポテンシャルがある。

Outline of Final Research Achievements

In this study, we propose a new tracer called “atmospheric nitrate concentration”, which can be calculated from both concentration and triple oxygen isotopic composition of dissolved nitrate in river water, to determine the removal rate of nitrate (sum of denitrification rate and assimilation rate) in the river environments. We determine the in-situ denitrification, assimilation, and nitrification rates in the river water individually by tracing the atmospheric nitrate concentration together with total nitrate concentration. The results were compared with the denitrification / assimilation rate obtained by the conventional culture method. The new method is an epoch-making method that can quantify the denitrification rate of the entire river environment including the riverbed in the natural state without adding or culturing an artificial tracer.

Academic Significance and Societal Importance of the Research Achievements

本研究の目的は、河川環境下における脱窒・硝化・同化速度の時空間変化を正確に実測する手法を開発することである。従来の河川環境における脱窒速度の推定は、生態系モデルを使った研究例や、アセチレン阻害法や人工同位体トレーサー法といった培養法を使った研究例が多く、実際の河川環境で自然の状態のまま脱窒速度を実測する手法は無かった。本研究が提案する新手法は、天然トレーサーを使っており、自然の状態のまま河床を含めた河川環境全体の窒素循環速度を定量できる。この新手法が確立することで、河川環境の窒素浄化能力やその規定因子に対する知見が深まり、関連する環境学、工学、農学、水産学など多くの分野の発展に貢献する。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (26 results)

All 2022 2021 2020 2019 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 4 results) Presentation (18 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Journal Article] Tracing the source of nitrate enriched in a forested stream during storm events2022

    • Author(s)
      Ding, W., U. Tsunogai, F. Nakagawa, T. Sambuichi, H. Sase, M. Morohashi, and H. Yotsuyanagi
    • Journal Title

      Biogeosciences

      Volume: -

    • DOI

      10.5194/bg-2022-30

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 赤外分光法による軽元素安定同位体比の分析2022

    • Author(s)
      角皆 潤, 中川 書子
    • Journal Title

      澤田嗣郎監修「先端の分析法 第2版」

      Volume: - Pages: 250-254

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitrogen saturation of forested catchments in central Japan - Progress or recovery?2021

    • Author(s)
      Sase Hiroyuki、Takahashi Masamichi、Matsuda Kazuhide、Yamashita Naoyuki、Tsunogai Urumu、Nakagawa Fumiko、Morohashi Masayuki、Yotsuyanagi Hiroki、Ohizumi Tsuyoshi、Sato Keiichi、Kurokawa Junichi、Nakata Makoto
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 68 Issue: 1 Pages: 5-14

    • DOI

      10.1080/00380768.2021.1991228

    • NAID

      210000159409

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vertical Changes in the Flux of Atmospheric Nitrate From a Forest Canopy to the Surface Soil Based on Δ17O Values2021

    • Author(s)
      Inoue Takahiro, Nakagawa Fumiko, Shibata Hideaki, Tsunogai Urumu
    • Journal Title

      Journal of Geophysical Research: Biogeosciences

      Volume: 126 Issue: 4

    • DOI

      10.1029/2020jg005876

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Determination of the triple oxygen isotopic composition of tropospheric ozone in terminal positions using a multistep nitrite-coated filter-pack system.2021

    • Author(s)
      Xu, H., U. Tsunogai, F. Nakagawa, Y. Li, M. Ito, K. Sato, and H. Tanimoto
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: 35 Issue: 15

    • DOI

      10.1002/rcm.9124

    • NAID

      120007183210

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-precision Δ′17O measurements of geothermal H2O and MORB on the VSMOW-SLAP scale: evidence for active oxygen exchange between the lithosphere and hydrosphere.2021

    • Author(s)
      Sambuichi, T., U. Tsunogai, K. Kura, F. Nakagawa, and T. Ohba
    • Journal Title

      Geochemical Journal

      Volume: 55 Issue: 6 Pages: e25-e33

    • DOI

      10.2343/geochemj.2.0644

    • NAID

      130008119591

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dual stable isotope characterization of excess methane in oxic waters of a mesotrophic lake2020

    • Author(s)
      Tsunogai Urumu、Miyoshi Yuko、Matsushita Toshiyuki、Komatsu Daisuke D.、Ito Masanori、Sukigara Chiho、Nakagawa Fumiko、Maruo Masahiro
    • Journal Title

      Limnology and Oceanography

      Volume: 65 Issue: 12 Pages: 2937-2952

    • DOI

      10.1002/lno.11566

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 降雨に伴う渓流水硝酸濃度増大の原因解明とその大気硝酸直接流出率算出への影響評価2021

    • Author(s)
      丁 イ天, 角皆 潤, 中川 書子, 佐瀬 裕之, 諸橋 将雪, 四柳 宏基
    • Organizer
      JpGU Meeting 2021
    • Related Report
      2021 Annual Research Report
  • [Presentation] Quantifying denitrification rate across water-sediment interface in aquatic areas by using triple oxygen isotopes as tracers2021

    • Author(s)
      フェイ 粹霞, 中川 書子, 角皆 潤, 伊藤 昌稚, 水野 広香
    • Organizer
      JpGU Meeting 2021
    • Related Report
      2021 Annual Research Report
  • [Presentation] 三酸素同位体組成を指標に用いた対流圏ガス状亜硝酸の起源解明2021

    • Author(s)
      中川 書子, 頼 鵬, 平野 一哉, 伊藤 昌稚, 角皆 潤
    • Organizer
      JpGU Meeting 2021
    • Related Report
      2021 Annual Research Report
  • [Presentation] High-precision measurement of 17O-excess in phosphate in hydrosphere2021

    • Author(s)
      三歩一 孝, 角皆 潤, 中川 書子, 伊藤 昌稚
    • Organizer
      JpGU Meeting 2021
    • Related Report
      2021 Annual Research Report
  • [Presentation] 光量の違いが表層水中の酸素消費速度に及ぼす影響2021

    • Author(s)
      大石 雄太, 角皆 潤, 中川 書子, 伊藤 昌稚
    • Organizer
      日本地球化学会第68回年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 土壌由来一酸化二窒素の三酸素同位体組成2021

    • Author(s)
      黄 天政, 角皆 潤, Kim Yongwon,中川 書子, 伊藤 昌稚
    • Organizer
      日本地球化学会第68回年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 現場環境下における都市河川中の脱窒・同化・硝化速度定量法開発2020

    • Author(s)
      中川 書子, 鈴木 謙介, 伊藤 昌稚, 角皆 潤
    • Organizer
      JpGU-AGU Joint Meeting 2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] 安定同位体組成を指標に用いた伊勢湾における過飽和メタンの起源および挙動解明2020

    • Author(s)
      蘭 慧, 角皆 潤, 中川 書子, 伊藤 昌稚, 三好 友子, 原 修一
    • Organizer
      JpGU-AGU Joint Meeting 2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] 対流圏一酸化窒素の窒素及び三酸素同位体組成の定量2020

    • Author(s)
      平野一哉, 角皆 潤, 中川書子, 伊藤昌稚
    • Organizer
      第25回大気化学討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 多段フィルターパック法を用いたガス状亜硝酸および二酸化窒素の三酸素同位体異常定量2020

    • Author(s)
      頼 鵬, 中川書子, 角皆 潤, 丁 とう, 野口 泉, 山口高志
    • Organizer
      2020年度日本地球化学会第67回オンライン年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] The triple oxygen isotopes of nitrate as tracer of atmospheric nitrate deposition in coastal seawater2019

    • Author(s)
      Fumiko Nakagawa, Urumu Tsunogai, Takuya Ohyama, Daisuke D. Komatsu, Atsushi Kubo, and Jota Kanda
    • Organizer
      SOLAS Open Science Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The 17O-excess of nitrate in the Japan Sea2019

    • Author(s)
      Urumu Tsunogai, Peng Weiqing, Masanori Ito, Chiho Sukigara, D.D. Komatsu, and Fumiko Nakagawa
    • Organizer
      SOLAS Open Science Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 大気硝酸添加培養法に基づく都市河川における河床窒素循環速度の季節変化定量2019

    • Author(s)
      中川書子, 山本雄大, 池上文香, 角皆 潤
    • Organizer
      2019年度日本地球化学会第66回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 現場環境下における河川硝酸除去速度の時間変化定量法開発2019

    • Author(s)
      鈴木謙介,角皆 潤,中川書子,伊藤昌稚
    • Organizer
      2019年度日本地球化学会第66回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 水環境中の溶存無機態リン酸の三酸素同位体組成定量2019

    • Author(s)
      三歩一孝, 角皆 潤, 中川書子, 伊藤昌稚
    • Organizer
      2019年度日本地球化学会第66回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 降水用自動サンプラーの試作及びこれを用いた短時間化学組成変化の定量化2019

    • Author(s)
      嶺野雄登, 角皆潤, 鈴木謙介, 中川書子, 高橋菜椿子
    • Organizer
      第24回大気化学討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 対流圏二酸化窒素の三酸素同位体組成定量2019

    • Author(s)
      中川書子, 丁トウ, 角皆 潤, 山口高志, 野口 泉
    • Organizer
      日本地球惑星科学連合2019年大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 都市大気中二酸化窒素および亜硝酸ガスの三酸素同位体組成の時間変化2019

    • Author(s)
      中川書子・丁トウ・頼鵬・平野一哉・角皆潤,野口泉・山口高志
    • Organizer
      第24回大気化学討論会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 名古屋大学 環境学研究科 地球環境科学専攻 大気水圏科学系 生物地球化学グループ

    • URL

      http://biogeochem.has.env.nagoya-u.ac.jp

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

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