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A new view of methanogenesis with a function-specific biomarker

Research Project

Project/Area Number 19H04261
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 63010:Environmental dynamic analysis-related
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Kaneko Masanori  国立研究開発法人産業技術総合研究所, 地質調査総合センター, 主任研究員 (80633239)

Co-Investigator(Kenkyū-buntansha) 延 優 (NOBUMASARU・KONISHI)  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (40805644)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywordsメタン菌 / 補酵素F430 / メチルコエンザイムM還元酵素 / 海底堆積物 / メタン生成アーキア / 遺伝子解析 / メタン生成菌 / メタゲノム解析 / 海洋堆積物 / 未知機能
Outline of Research at the Start

本研究の目的は,①補酵素F430の分布解析により、海洋堆積物表層から生命の増殖温度限界を超える基盤岩までのメタン生成菌の分布を明らかにし,メタン生成菌の存在限界およびそれを支配する環境要因の解明をすること,②未知メタン菌の存在および多様性を明らかにすることである。本研究の成功は,「どこに,どれくらいメタン生成菌が存在するのか」という長年の学術的問いに解を与える。また,微生物による地球表層の炭素循環において,時空間的議論を可能にするだけでなく,メタン生成菌の多様性と初期生命のありようについて新たな知見をもたらし,全く新しいメタン生成観の構築が期待できる。

Outline of Final Research Achievements

The objectives of this research were 1) to clarify the distribution of methanogens from the surface of marine sediments to basement rocks and to identify environmental factors controlling their distribution, and 2) to prove the link between the unknown function of coenzyme F430 and the unknown methanogens.
The distribution and kinetics of non-biological degradation of coenzyme F430 up to 2.5 km below the sediment off the Shimokita Peninsula were clarified, and the distribution of methanogens in the deep marine sediment was quantitatively clarified for the first time. The methanogenic biomass was 1-2 orders of magnitude higher than estimation based on genome analysis, indicating the possible presence of unknown methanogens.we also clarified the unknown function of coenzyme F430, and generated new insights into the evolution of methanogens.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は「メタン菌はどこに、どれくらいいるのか」という長年の学術的問いに対し、初めて定量的にメタン菌の分布を明らかにした。また、そこから未知メタン菌の存在の可能性について示した。
補酵素F430の未知機能について明らかにし、触媒としての可能性と、未知機能を持ったメタン菌の探索に資する結果を得た。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (12 results)

All 2023 2022 2021 2020 Other

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 6 results) Presentation (3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] The origin and evolution of methanogenesis and<i>Archaea</i>are intertwined2023

    • Author(s)
      Mei Ran、Kaneko Masanori、Imachi Hiroyuki、Nobu Masaru K
    • Journal Title

      PNAS Nexus

      Volume: 2 Issue: 2 Pages: 1-10

    • DOI

      10.1093/pnasnexus/pgad023

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 最先端の研究開発(産業技術総合研究所)第1回「メタンハイドレート胚胎域の南海トラフ海底堆積物で生きるメタン生成微生物の実態の解明」2022

    • Author(s)
      吉岡 秀佳, 片山 泰樹, 金子 雅紀, 坂田 将, 天羽 美紀, 藤井 哲哉
    • Journal Title

      日本原子力学会誌「アトモス」

      Volume: 64 Pages: 24-27

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Cultivation and biogeochemical analyses reveal insights into methanogenesis in deep subseafloor sediment at a biogenic gas hydrate site2022

    • Author(s)
      Katayama Taiki、Yoshioka Hideyoshi、Kaneko Masanori、Amo Miki、Fujii Tetsuya、Takahashi Hiroshi A.、Yoshida Satoshi、Sakata Susumu
    • Journal Title

      The ISME Journal

      Volume: 16 Issue: 5 Pages: 1464-1472

    • DOI

      10.1038/s41396-021-01175-7

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insights into the Methanogenic Population and Potential in Subsurface Marine Sediments Based on Coenzyme F430 as a Function-Specific Biomarker2021

    • Author(s)
      Kaneko Masanori、Takano Yoshinori、Kamo Masashi、Morimoto Kazuya、Nunoura Takuro、Ohkouchi Naohiko
    • Journal Title

      JACS Au

      Volume: 1 Issue: 10 Pages: 1743-1751

    • DOI

      10.1021/jacsau.1c00307

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis2021

    • Author(s)
      Urai Atsushi、Matsushita Makoto、Park Ho-Dong、Imachi Hiroyuki、Ogawara Miyuki、Iwata Hiroki、Kaneko Masanori、Ogawa Nanako O.、Ohkouchi Naohiko、Takano Yoshinori
    • Journal Title

      Progress in Earth and Planetary Science

      Volume: 8 Issue: 1 Pages: 1-17

    • DOI

      10.1186/s40645-021-00450-7

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections2021

    • Author(s)
      Suda Konomi、Ikarashi Masayuki、Tamaki Hideyuki、Tamazawa Satoshi、Sakata Susumu、Haruo Maeda、Kamagata Yoichi、Kaneko Masanori、Ujiie Tomomi、Shinotsuka Yumi、Wakayama Tatsuki、Iwama Hiroki、Osaka Noriko、Mayumi Daisuke、Yonebayashi Hideharu
    • Journal Title

      Journal of Petroleum Science and Engineering

      Volume: 201 Pages: 108458-108458

    • DOI

      10.1016/j.petrol.2021.108458

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Temperature limits to deep subseafloor life in the Nankai Trough subduction zone2020

    • Author(s)
      Heuer Verena B.、Inagaki Fumio、Morono Yuki、Kubo Yusuke、Spivack Arthur J.、Viehweger Bernhard、Treude Tina、Beulig Felix、Schubotz Florence、Tonai Satoshi、Bowden Stephen A.、Cramm Margaret、Henkel Susann、Hirose Takehiro、Homola Kira、Hoshino Tatsuhiko、Ijiri Akira、Imachi Hiroyuki、Kamiya Nana、et al.
    • Journal Title

      Science

      Volume: 370 Issue: 6521 Pages: 1230-1234

    • DOI

      10.1126/science.abd7934

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 東部南海トラフ海底堆積物の微生物メタン生成活動2022

    • Author(s)
      吉岡 秀佳, 片山 泰樹, 金子 雅紀, 坂田 将, 天羽 美紀, 藤井 哲哉
    • Organizer
      JpGU Meeting 2022
    • Related Report
      2021 Annual Research Report
  • [Presentation] 日本海酒田海丘における掘削調査で発見されたメタンハイドレートと堆積物間隙水の地球化学的特徴2022

    • Author(s)
      宮嶋 佑典, 太田 雄貴, 青木 伸輔, 金子 雅紀, 吉岡 秀佳, 高橋 浩, 井川 怜欧, 町田 功, 棚橋 学, 鈴木 清史, 佐藤 幹夫
    • Organizer
      JpGU Meeting 2022
    • Related Report
      2021 Annual Research Report
  • [Presentation] 東部南海トラフ海底下堆積物における生物的メタン生成2021

    • Author(s)
      吉岡 秀佳、片山 泰樹、金子 雅紀、坂田 将
    • Organizer
      砂層型メタンハイドレートフォーラム2021
    • Related Report
      2020 Annual Research Report
  • [Remarks] 南海トラフの深海底堆積物で生きるメタン生成微生物の特徴を解明

    • URL

      https://www.aist.go.jp/aist_j/press_release/pr2022/pr20220202/pr20220202.html

    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] メタン生成補酵素を用いた電気化学的バイオガス生産システム2020

    • Inventor(s)
      金子雅紀、辻村清也
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
    • Overseas

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

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