Role of non-apoptotic caspase in environmental electrophile-induced disorder of central nervous system

• Project/Area Number: 19H04279
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 63030:Chemical substance influence on environment-related
• Research Institution: Tokyo University of Science
• Principal Investigator: Ichihara Gaku 東京理科大学, 薬学部薬学科, 教授 (90252238)
• Co-Investigator(Kenkyū-buntansha): 宗 才 東京理科大学, 薬学部薬学科, 助教 (20817267)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2021: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2020: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: カスパーゼ / 神経変性 / 炎症 / 酸化ストレス / 神経突起 / ミクログリア / 神経毒性 / 非アポトーシス / 中枢神経 / 毒性 / 親電子性物質

Outline of Research at the Start

本研究は環境化学物質のハザード評価においてこれまで見逃されてきた中枢神経障害の同定を可能にするとともに、アポトーシスと強く結びつけられて議論されることが多かったカスパーゼ－の非アポトーシス機能の解明およびその制御機構を明らかにする。本研究の成果は、環境化学物質による中枢神経障害だけでなく、広汎な神経変性疾患における非アポトーシス誘導性カスパーゼ－の役割の解明に資する。

Outline of Final Research Achievements

The study demonstrated that exposure to acrylamide induced expression of initiator caspase 8, inflammatory caspase 1 and 4 and proinflammatory cytokines. These results suggest receptor-mediated extrinsic pathway of cell death, not intrinsic pathway, is operating in acrylamide-exposed neurons, and intercellular signals from microglia play important role in neurite retraction. The study also shows that caspase 6, which is an effector caspase, in neurons is involved in acrylamide-induced neurodegeneration. The study demonstrates neuronal caspases are involved in neurite retraction and neurodegeneration without induction of neuronal apoptosis.

Academic Significance and Societal Importance of the Research Achievements

本研究は環境化学物質のハザード評価においてこれまで見逃されてきた中枢神経障害の同定を可能にするとともに、アポトーシスと強く結びつけられて議論されることが多かったカスパーゼ－の非アポトーシス機能が環境化学物質による神経障害に関与すること、ミクログリアからニューロンへの細胞間シグナルが神経変性誘導に貢献していることを明らかにした。本研究は産業現場における自律的な化学物質のリスク管理が求められている状況に対し、未知の化学物質の中枢神経毒性を予見するための有効なツール構築の基礎を提供する。

Research Products

• [Int'l Joint Research] University of Paris V Descartes(フランス)
• [Journal Article] Higher viral load and infectivity increase risk of aerosol transmission for Delta and Omicron variants of SARS-CoV-2 (2022)
  • Author(s): Michael Riediker, Leonardo Briceno-Ayala, Gaku Ichihara, Daniele Albani, Deyan Poffet, Dai-Hua Tsai, Samuel Iff, Christian Monn
  • Journal Title: Swiss Medical Weekly Volume: 152 Issue: 0102 Pages: w30133-w30133
  • DOI: 10.4414/smw.2022.w30133
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Factory site analysis of respirable fibers generated during the process of cutting and grinding of carbon fibers-reinforced plastics (2022)
  • Author(s): Kumoi Jun、Ikegami Akihiko、Fujitani Yuji、Morikawa Kota、Ichihara Gaku、Yano Takeo、Ichihara Sahoko
  • Journal Title: International Archives of Occupational and Environmental Health Volume: - Issue: 7 Pages: 1557-1565
  • DOI: 10.1007/s00420-022-01840-0
  • Peer Reviewed
• [Journal Article] Genetic ablation of Nrf2 exacerbates neurotoxic effects of acrylamide in mice (2021)
  • Author(s): Ekuban Frederick Adams、Zong Cai、Takikawa Madoka、Morikawa Kota、Sakurai Toshihiro、Ichihara Sahoko、Itoh Ken、Yamamoto Masayuki、Ohsako Seiichiroh、Ichihara Gaku
  • Journal Title: Toxicology Volume: 456 Pages: 152785-152785
  • DOI: 10.1016/j.tox.2021.152785
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nrf2 Activation Attenuates Acrylamide-Induced Neuropathy in Mice (2021)
  • Author(s): Davuljigari Chand Basha、Ekuban Frederick Adams、Zong Cai、Fergany Alzahraa A. M.、Morikawa Kota、Ichihara Gaku
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 11 Pages: 5995-5995
  • DOI: 10.3390/ijms22115995
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of Macrophages in Cytotoxicity, Reactive Oxygen Species Production and DNA Damage in 1,2-Dichloropropane-Exposed Human Cholangiocytes In Vitro (2021)
  • Author(s): Ekuban Abigail、Zong Cai、Ekuban Frederick Adams、Kimura Yusuke、Takizawa Ryoya、Morikawa Kota、Kinoshita Kazuo、Ichihara Sahoko、Ohsako Seiichiroh、Ichihara Gaku
  • Journal Title: Toxics Volume: 9 Issue: 6 Pages: 128-128
  • DOI: 10.3390/toxics9060128
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 1,2-Dichloropropane induces γ-H2AX expression in human cholangiocytes only in the presence of macrophages (2021)
  • Author(s): Takizawa Ryoya、Ichihara Sahoko、Zong Cai、Kinoshita Kazuo、Sakurai Toshihiro、Ikegami Akihiko、Mise Nathan、Ichihara Gaku
  • Journal Title: Toxicology Letters Volume: 349 Pages: 134-144
  • DOI: 10.1016/j.toxlet.2021.06.009
  • Peer Reviewed
• [Journal Article] Environmental arsenic exposure and its contribution to human diseases, toxicity mechanism and management (2021)
  • Author(s): Rahaman Md. Shiblur、Rahman Md. Mostafizur、Mise Nathan、Sikder Md. Tajuddin、Ichihara Gaku、Uddin Md. Khabir、Kurasaki Masaaki、Ichihara Sahoko
  • Journal Title: Environmental Pollution Volume: 289 Pages: 117940-117940
  • DOI: 10.1016/j.envpol.2021.117940
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Occupational exposure limits for acetaldehyde, 2-bromopropane, glyphosate, manganese and inorganic manganese compounds, and zinc oxide nanoparticle, and the biological exposure indices for cadmium and cadmium compounds and ethylbenzene, and carcinogenicity, occupational sensitizer, and reproductive toxicant classifications (2021)
  • Author(s): The Committee for Recommendation of Occupational Exposure Limits, Japan Society for Occupational Health
  • Journal Title: Journal of Occupational Health Volume: 63 Issue: 1 Pages: n/a
  • DOI: 10.1002/1348-9585.12294
  • NAID: 130008144183
  • ISSN: 1341-9145, 1348-9585
  • Peer Reviewed / Open Access
• [Journal Article] Recovery scenario and immunity in COVID-19 disease: A new strategy to predict the potential of reinfection (2021)
  • Author(s): Khoshkam Zahra、Aftabi Younes、Stenvinkel Peter、Paige Lawrence B.、Rezaei Mehran Habibi、Ichihara Gaku、Fereidouni Sasan
  • Journal Title: Journal of Advanced Research Volume: - Pages: 49-60
  • DOI: 10.1016/j.jare.2020.12.013
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Functionalized surface-charge SiO2 nanoparticles induce pro-inflammatory responses, but are not lethal to Caco-2 cells. (2020)
  • Author(s): Tada-Oikawa S, Eguchi M, Yasuda MT, Izuoka K, Ikegami A, Vranic S, Boland S, Tran L, Ichihara G, Ichihara S
  • Journal Title: Chem Res Toxicol Volume: 印刷中 Issue: 5 Pages: 1226-1236
  • DOI: 10.1021/acs.chemrestox.9b00478
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification by TCGA database search of five genes that are aberrantly expressed and involved in hepatocellular carcinoma potentially via DNA methylation changes (2020)
  • Author(s): Matsushita Junya、Suzuki Takehiro、Okamura Kazuyuki、Ichihara Gaku、Nohara Keiko
  • Journal Title: Environmental Health and Preventive Medicine Volume: 25 Issue: 1 Pages: 31-31
  • DOI: 10.1186/s12199-020-00871-8
  • NAID: 50014448484
  • Peer Reviewed / Open Access
• [Journal Article] Proteomic analysis of liver proteins of mice exposed to 1,2-dichloropropane. (2020)
  • Author(s): Zhang X, Morikawa K, Mori Y, Zong C, Zhang L, Garner E, Huang C, Wu W, Chang J, Nagashima D, Sakurai T, Ichihara S, Oikawa S, Ichihara G.
  • Journal Title: Arch Toxicol. Volume: 94 Issue: 8 Pages: 2691-2705
  • DOI: 10.1007/s00204-020-02785-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An introduction to EpiPol (Epigenetic affecting Polymorphism) concept with an in silico identification of CpG-affecting SNPs in the upstream regulatory sequences of human AHR gene (2020)
  • Author(s): Aftabi Younes、Khoshkam Zahra、Amiri-Sadeghan Amir、Khalili Yeganeh、Ichihara Gaku
  • Journal Title: Meta Gene Volume: 26 Pages: 100805-100805
  • DOI: 10.1016/j.mgene.2020.100805
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Exposure to acrylamide decreases noradrenergic axons in rat brain (2020)
  • Author(s): Zhang Lingyi、Hara Satoshi、Ichinose Hiroshi、Nagashima Daichi、Morita Kyo、Sakurai Toshihiro、Ichihara Sahoko、Ichihara Gaku
  • Journal Title: NeuroToxicology Volume: 78 Pages: 127-133
  • DOI: 10.1016/j.neuro.2020.03.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Occupational exposure limits for cumene, 2,4-dichlorophenoxy acetic acid, silicon carbide whisker, benzyl alcohol, and methylamine, and carcinogenicity, occupational sensitizer, and reproductive toxicant classifications (2019)
  • Author(s): The Committee for Recommendation of Occupational Exposure Limits, Japan Society for Occupational Health
  • Journal Title: Journal of Occupational Health Volume: 61 Issue: 4 Pages: 328-330
  • DOI: 10.1002/1348-9585.12073
  • NAID: 130007683730
  • ISSN: 1341-9145, 1348-9585
  • Year and Date: 2019-07-20
  • Peer Reviewed / Open Access
• [Journal Article] Urinary trimethyl tin reflects blood trimethyl tin in workers recycling organotins (2019)
  • Author(s): Ichihara Gaku、Iida Mayu、Watanabe Eri、Fujie Tomoya、Kaji Toshiyuki、Lee Eunmi、Kim Yangho
  • Journal Title: Journal of Occupational Health Volume: 61 Issue: 3 Pages: 257-260
  • DOI: 10.1002/1348-9585.12052
  • NAID: 130007653262
  • ISSN: 1341-9145, 1348-9585
  • Year and Date: 2019-05-20
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pyrrole adducts in globin and plasma of workers exposed to hexane (2019)
  • Author(s): Ichihara Gaku、Amarnath Venkataraman、Valentine Holly L.、Takeshita Tatsuya、Morimoto Kanehisa、Sobue Tomotaka、Kawai Toshio、Valentine William M.
  • Journal Title: International Archives of Occupational and Environmental Health Volume: 印刷中 Issue: 6 Pages: 873-881
  • DOI: 10.1007/s00420-019-01430-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Proteomic analysis of hippocampal proteins in acrylamide-exposed Wistar rats (2019)
  • Author(s): Nagashima D, Zhang L, Kitamura Y, Ichihara S, Watanabe E, Zong C, Yamano Y, Sakurai T, Oikawa S, Ichihara G
  • Journal Title: Archives of Toxicology Volume: 印刷中 Issue: 7 Pages: 1993-2006
  • DOI: 10.1007/s00204-019-02484-9
  • Peer Reviewed / Open Access
• [Journal Article] Ablation of aryl hydrocarbon receptor promotes angiotensin II-induced cardiac fibrosis through enhanced c-Jun/HIF-1α signaling (2019)
  • Author(s): Ichihara S, Li P, Mise N, Suzuki Y, Izuoka K, Nakajima T, Gonzalez F, Ichihara G.
  • Journal Title: Archives of Toxicology Volume: 印刷中 Issue: 6 Pages: 1543-1553
  • DOI: 10.1007/s00204-019-02446-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Particle toxicology and health - where are we? (2019)
  • Author(s): Riediker M, Zink D, Kreyling W, Oberdorster G, Elder A, Graham U, Lynch I, Duschl A, Ichihara G, Ichihara S, Kobayashi T, Hisanaga N, Umezawa M, Cheng TJ, Handy R, Gulumian M, Tinkle S, Cassee F
  • Journal Title: Part Fibre Toxicol Volume: 印刷中 Issue: 1 Pages: 19-19
  • DOI: 10.1186/s12989-019-0302-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of microglial activation and neuroinflammation in neurotoxicity of acrylamide in vivo and in vitro (2019)
  • Author(s): Zong Cai, Hasegawa Rieka, Urushitani Makoto, Zhang Lingyi, Nagashima Daichi, Sakurai Toshihiro, Ichihara Sahoko, Ohsako Seiichiro, Ichihara Gaku
  • Journal Title: Archives of Toxicology Volume: 印刷中 Issue: 7 Pages: 2007-2019
  • DOI: 10.1007/s00204-019-02471-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Drosophila melanogaster as an in vivo model to study the potential toxicity of cerium oxide nanoparticles (2019)
  • Author(s): Sundararajan Vignesh、Dan Pallavi、Kumar Ajay、Venkatasubbu G. Devanand、Ichihara Sahoko、Ichihara Gaku、Sheik Mohideen Sahabudeen
  • Journal Title: Applied Surface Science Volume: 490 Pages: 70-80
  • DOI: 10.1016/j.apsusc.2019.06.017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Correction to: Particle toxicology and health - where are we? (2019)
  • Author(s): Riediker Michael、Zink Daniele、Kreyling Wolfgang、Oberd?rster G?nter、Elder Alison、Graham Uschi、Lynch Iseult、Duschl Albert、Ichihara Gaku、Ichihara Sahoko、Kobayashi Takahiro、Hisanaga Naomi、Umezawa Masakazu、Cheng Tsun-Jen、Handy Richard、Gulumian Mary、Tinkle Sally、Cassee Flemming
  • Journal Title: Particle and Fibre Toxicology Volume: 16 Issue: 1 Pages: 26-26
  • DOI: 10.1186/s12989-019-0308-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of Nrf2 in inflammatory response in lung of mice exposed to zinc oxide nanoparticles (2019)
  • Author(s): Sehsah Radwa、Wu Wenting、Ichihara Sahoko、Hashimoto Naozumi、Hasegawa Yoshinori、Zong Cai、Itoh Ken、Yamamoto Masayuki、Elsayed Ahmed Ali、El-Bestar Soheir、Kamel Emily、Ichihara Gaku
  • Journal Title: Particle and Fibre Toxicology Volume: 16 Issue: 1 Pages: 47-47
  • DOI: 10.1186/s12989-019-0328-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 薬局・薬剤師に望む感染症へのアプローチ (2019)
  • Author(s): 長嶋大地、山内武紀、市原学、山野優子
  • Journal Title: 医学と薬学/自然科学社 Volume: 76 Pages: 471-476
• [Presentation] 環境労働衛生学における実験医学の役割 (2022)
  • Author(s): 市原 学
  • Organizer: 第21回分子予防環境医学研究会
  • Invited
• [Presentation] 1,2-ジクロロプロパンの毒性発現におけるNRF2の役割の検討 (2022)
  • Author(s): 木村優介、指宿大介、フレデリックアダムズエクバン、宗才、市原学
  • Organizer: 第21回分子予防環境医学研究会
• [Presentation] Role of IL-1β in Acrylamide-induced Neurotoxicity in Mice (2022)
  • Author(s): Alzahraa A.M. Fergnay, Frederick Adams Ekuban, Cai Zong, Aina Suzuki, Gaku Ichihara
  • Organizer: 第21回分子予防環境医学研究会
• [Presentation] ケモカイン受容体CCR2の細胞内領域ペプチドは細胞死を誘導する (2022)
  • Author(s): 八島拓巳, 遠田悦子, 小窪香菜, 市原学, 松島綱治, 寺島裕也
  • Organizer: 第21回分子予防環境医学研究会
• [Presentation] Arsenic induces hypertension by suppressing the vasoprotective axes of the renin-angiotensin system (2022)
  • Author(s): Md Shiblur Rahaman, Nathan Mise, Cai Zong, Gaku Ichihara, Sahoko Ichihara
  • Organizer: 第21回分子予防環境医学研究会
• [Presentation] 1,2-ジクロロプロパンの毒性発現におけるNRF2の役割の検討 (2022)
  • Author(s): 木村 優介、指宿 大介、フレデリック アダムズ エクバン、宗 才、伊東 健、山本 雅之、市原 学
  • Organizer: 第92回日本衛生学会学術総会
• [Presentation] 素による血圧上昇におけるレニン‐アンギオテンシン系の役割の解明 (2022)
  • Author(s): ラハマン エムディー シブルール、三瀬 名丹、宗 才、市原 学、市原 佐保子
  • Organizer: 第92回日本衛生学会学術総会
• [Presentation] Debris from carbon fiber reinforced plastics (CFRP) in an occupational setting (2022)
  • Author(s): Akihiko Ikegami, Jun Kumoi, Yuji Fujitami, Gaku Ichihara, Takeo Yano, Sahoko Ichihara
  • Organizer: 33rd International Congress on Occupational Health (ICPH 2022)
  • Int'l Joint Research
• [Presentation] Effect of Nrf2 deletion on inflammatory response in lung of female mice exposed to zinc oxide nanoparticles (2021)
  • Author(s): Sehsah R, Wu W, Ichihara S, Hashimoto N, Hasegawa Y, Zong C, Yamazaki K, Sato H, Itoh K, Yamamoto M, Ali Elsayed A, El-Bestar S, Kamel E, Ichihara G
  • Organizer: NanoTox2021
  • Int'l Joint Research
• [Presentation] Pulmonary effects of Fe3O4-PEG-PLGA nanoparticles in human bronchial epithelial cells and in wild type and Nrf2 knockout mice following pharyngeal aspiration (2021)
  • Author(s): Sato H, Zong C, Devineau S, McCord C, Ichihara S, Brookes O, Ekuban F, Itoh K, Yamamoto M, Boland S, Baeza-Squiban A, Ichihara G
  • Organizer: NanoTox2021
  • Int'l Joint Research
• [Presentation] 炭素繊維強化プラスチック製造加工現場の繊維状物質 (2021)
  • Author(s): 雲井 純、池上 昭彦、藤谷 雄二、市原 学、矢野 竹男、市原 佐保子
  • Organizer: 第94回日本産業衛生学会
• [Presentation] 東京理科大学における新型コロナウイルス感染予防対策 (2021)
  • Author(s): 市原 学、岩崎 等、北村 春幸、岡村 総一郎、井手本 康、小林 秀至、久保 聡、伊藤 亨子、坂本 那奈、鈴木 雅子、江口 泉、滝野 妙子、杉崎 芳子、小澤 亮、柴崎 伸明、東甫 伸一、清水 勉、伊藤 真紀子、入江 淑人、青木 良明、下山 佳子、名富 麻衣
  • Organizer: 第94回日本産業衛生学会
• [Presentation] 不織布マスク、フェイスシールド併用時における二酸化炭素濃度 (2021)
  • Author(s): 市原 佐保子、市原 学
  • Organizer: 第94回日本産業衛生学会
• [Presentation] Role of microglia in mediating neurite retraction of noradrenergic neurons following acrylamide exposure and transcriptomic analysis (2021)
  • Author(s): Cai ZONG, Harue SATO, Sho IWAMA, Schneider BENOIT, Makoto URUSHITANI, Shigeyuki SHICHINO, Kouji MATSUSHIMA, Seiichiroh OHSAKO, Gaku ICHIHARA
  • Organizer: 第48回日本毒性学会学術年会
• [Presentation] Sulforaphane Attenuates Acrylamide-induced Neuropathy in Mice (2021)
  • Author(s): Chand Basha DAVULJIGARI, Frederick Adams EKUBAN, Cai ZONG, Alzahraa A.M. FERGANY, Kota MORIKAWA, Gaku ICHIHARA
  • Organizer: 第48回日本毒性学会学術年会
• [Presentation] Role of macrophages in cytotoxicity, reactive oxygen species production and DNA damage in 1,2-dichloropropane-exposed human cholangiocytes in vitro (2021)
  • Author(s): Abigail EKUBAN, Cai ZONG, Frederick Adams EKUBAN, Yusuke KIMURA, Ryoya TAKIZAWA, Kota MORIKAWA, Kazuo KINOSHITA, Sahoko ICHIHARA, Seiichiroh OHSAKO, Gaku ICHIHARA
  • Organizer: 第48回日本毒性学会学術年会
• [Presentation] Pulmonary effects of Fe3O4-PEG-PLGA nanoparticles in wild type and Nrf2 knockout mice following pharyngeal aspiration (2021)
  • Author(s): Harue SATO, Cai ZONG, Stephanie DEVINEAU, Claire MCCORD, Sahoko ICHIHARA, Oliver BROOKES, Frederick Adams EKUBAN, Ken ITOH, Masayuki YAMAMOTO, Sonja BOLAND, Armelle BAEZA-SQUIBAN, Gaku ICHIHARA
  • Organizer: 第48回日本毒性学会学術年会
• [Presentation] Deletion of Nrf2 aggravates acrylamide-induced degeneration of monoaminergic axons in mice (2021)
  • Author(s): Frederick Adams Ekuban, Cai Zong, Madoka Takikawa, Kouta Morikawa, Toshihiro Sakurai, Sahoko Ichihara, Ken Itoh, Masayuki Yamamoto, Seiichiroh Ohsako, Gaku Ichihara
  • Organizer: 第44回 日本神経科学大会/第1回 CJK 国際会議
  • Int'l Joint Research
• [Presentation] Fe3O4-PEG-PLGA ナノ粒子の経気道曝露が肺にもたらす影響とNrf2 の役割 (2021)
  • Author(s): 佐藤春恵, 宗才, Stephanie Devineau, Claire McCord, 市原佐保子, Oliver Brookes, 伊東健, 山本雅之, Sonja Boland, Armelle Baeza-Squiban, 市原学
  • Organizer: フォーラム2021 衛生薬学・環境トキシコロジー
• [Presentation] フィプロニル曝露が引き起こすマウス脳海馬への影響 (2021)
  • Author(s): 安藤 宥理、宗 才、市原 学
  • Organizer: フォーラム2021 衛生薬学・環境トキシコロジー
• [Presentation] 炭素繊維強化プラスチック製造工場現場の微小粒子 (2021)
  • Author(s): 池上 昭彦、雲井 純、藤谷 雄二、矢野 竹男、市原 学、市原 佐保子
  • Organizer: 第48回産業中毒・生物学的モニタリング研究会
• [Presentation] Conditioned medium of ACR-exposed microglia induces neurite retraction of monoaminergic neurons (2021)
  • Author(s): Harue Sato, Cai Zong, Sho Iwama, Benoit Schneider, Makoto Urushitani, Gaku Ichihara
  • Organizer: 米国神経学会(SfN Global Connectome2021)
  • Int'l Joint Research
• [Presentation] Sulforaphane Attenuates Acrylamide-induced Neuropathy in Mice: Involvement of Nrf2 Pathway (2021)
  • Author(s): Chand Basha Davuljigari, Frederick Adams Ekuban, Cai Zong, Kota Morikawa, Alzahraa AM Fergany, Gaku Ichihara
  • Organizer: 第20回分子予防環境医学研究会
• [Presentation] Microglia mediates neurite retraction of monoaminergic neurons in acrylamide exposure (2021)
  • Author(s): Cai Zong, Harue Sato, Sho Iwama, Benoit Schneider, Makoto Urushitani, Shigeyuki Shichino, Kouji Matsushima, Seiichiro Ohsako, Gaku Ichihara
  • Organizer: 第20回分子予防環境医学研究会
• [Presentation] Genetic ablation of Nrf2 exacerbates neurotoxic effects of acrylamide in mice (2021)
  • Author(s): Frederick Adams Ekuban, Cai Zong, Madoka Takikawa, Kota Morikawa, Toshihiro Sakurai, Sahoko Ichihara, Ken Itoh, Masayuki Yamamoto, Seiichiroh Ohsako, Gaku Ichihara
  • Organizer: 第20回分子予防環境医学研究会
• [Presentation] Fe3O4-PEG-PLGA曝露によるヒト気管支上皮細胞と野生型および Nrf2欠損マウスの肺への影響 (2021)
  • Author(s): 佐藤 春恵、宗 才、Stephan ie Devineau, Claire McCord、市原 佐保子、Oliver Brookes、Frederick Adams Ekuban, 伊東 健、山本 雅之、Sonja Boland、Armelle Baeza Squiban、市原 学
  • Organizer: 第20回分子予防環境医学研究会
• [Presentation] Fe3O4-PEG-PLGAの経気道曝露によるマウスの肺への影響とNrf2の役割 (2021)
  • Author(s): 佐藤春恵、宗才、市原佐保子、伊東健、山本雅之、Boland Sonja、Baeza-Squiban Armelle、市原学
  • Organizer: 第91回日本衛生学会学術総会
• [Presentation] アクリルアミド曝露により誘導されるモノアミン神経変性のメカニズムに関する研究 (2021)
  • Author(s): 宗才、佐藤春恵、岩間聖、Benoit Schneider、漆谷真、市原学
  • Organizer: 第91回日本衛生学会学術総会
• [Presentation] 1,2-Dichloropropaneによる胆管細胞におけるDNA二重鎖切断の誘導機序の解明 (2021)
  • Author(s): 滝澤亮哉、市原佐保子、宗才、木下和生、櫻井敏博、池上明彦、三瀬名丹、市原学
  • Organizer: 第91回日本衛生学会学術総会
• [Presentation] Neurite retraction of monoaminergic neurons following exposure to conditioned medium of acrylamide-treated microglia and transcriptome analysis (2021)
  • Author(s): Cai Zong, Harue Sato, Sho Iwama, Benoit Schneider, Makoto Urushitani, Gaku Ichihara
  • Organizer: SOT2021（米国毒性学会）
  • Int'l Joint Research
• [Presentation] Pulmonary effects of Fe3O4-PEG-PLGA nanoparticles in human bronchial epothalial cells and in wild type and Nrf2 knockout mice following pharyngeal aspiration (2021)
  • Author(s): H. Sato, C. Zong, S. Devineau, C. McCord, S. Ichihara, O. Brookes, K.Itoh, M. Yamamoto, S. Boland, A. Baeza-Squiban, G. Ichihara
  • Organizer: SOT2021（米国毒性学会）
  • Int'l Joint Research
• [Presentation] 1,2-dichloropropane Induces γ-H2AX Expression in Human Cholangiocytes Only in the Presence of Macrophages (2021)
  • Author(s): R. Takizawa, C. Zong, K. Kinoshita, T. Sakurai, S. Ichihara, G. Ichihara
  • Organizer: SOT2021（米国毒性学会）
  • Int'l Joint Research
• [Presentation] Sulforaphane Attenuates Acrylamide-Induced Neuropathy in Mice: Involvement of Nrf2 Pathway (2021)
  • Author(s): C. B. Davuljigari, F. A. Ekuban, C. Zong, A. A. Fergany, G. Ichihara
  • Organizer: SOT2021（米国毒性学会）
  • Int'l Joint Research
• [Presentation] Neurite retraction of monoaminergic neurons following exposure to conditioned medium of acrylamide-treated microglia and transcriptome analysis (2021)
  • Author(s): 宗才、佐藤春恵、岩間聖、Benoit Schneider、漆谷真、市原学
  • Organizer: 第141回日本薬学会
• [Presentation] Fe3O4-PEG-PLGA経気道曝露による野生型およびNrf2欠損マウスの肺への影響 (2021)
  • Author(s): 佐藤春恵、宗才、市原佐保子、伊東健、山本雅之、Boland Sonja、Baeza-Squiban Armelle、市原学
  • Organizer: 第141回日本薬学会
• [Presentation] 肺胞オルガノイドを用いた肺線維症のモデル化 (2021)
  • Author(s): 松清里奈、小川達郎、七野成之、白石一茂、上羽悟史、市原学、松島鋼治
  • Organizer: 第141回日本薬学会
• [Presentation] 炭素繊維強化プラスチックの製造加工現場で発生する微小粒子の個人曝露評価 (2020)
  • Author(s): 雲井 純、池上 昭彦、松見 豊、藤谷 雄二、矢野 竹男、市原 学、市原 佐保子
  • Organizer: 第93回日本産業衛生学会
• [Presentation] 中枢神経毒性を有する新電子性物質はノルアドレナリン作動性神経を減少させる (2020)
  • Author(s): 長嶋 大地、張 霊逸、原 怜、一瀬 宏、市原 佐保子、山野 優子、市原 学
  • Organizer: 第93回日本産業衛生学会
• [Presentation] Protective effect of sulforaphane against acrylamide-induced neuropathy in adult mice (2020)
  • Author(s): Chand Basha DAVULJIGARI, Frederick Adams EKUBAN, Cai ZONG, Kouta MORIKAWA, Gaku ICHIHARA
  • Organizer: 第47回日本毒性学会学術年会
• [Presentation] Role of reactive oxygen species in cholangiocarcinoma induced by exposure to 1,2-dichloropropane in humans (2020)
  • Author(s): Abigail EKUBAN, Cai ZONG, Sahoko ICHIHARA, Seiichiroh OHSAKO, Gaku ICHIHARA
  • Organizer: 第47回日本毒性学会学術年会
• [Presentation] マウスにおけるNrf2の欠損はアクリルアミドの神経毒性に対する感受性を高める (2020)
  • Author(s): Frederick Adams EKUBAN, 宗 才, 瀧川 円賀, 森川 浩太, 櫻井 敏博, 市原 佐保子, 伊東 健, 山本 雅之, 大迫 誠一郎, 市原 学
  • Organizer: 第47回日本毒性学会学術年会
• [Presentation] アクリルアミド曝露によるバイオアミン神経変性におけるミクログリアの役割 (2020)
  • Author(s): 佐藤 春恵, 宗 才, 岩間 聖, Benoit SCHNEIDER, 漆谷 真, 市原 学
  • Organizer: 第47回日本毒性学会学術年会
• [Presentation] アルカリ性条件下1,2-ジクロロプロパンはヒト胆管細胞にγH2AXを誘導する (2020)
  • Author(s): 木村 優介, 宗 才, 大迫 誠一郎, 市原 学
  • Organizer: 第47回日本毒性学会学術年会
• [Presentation] Ahr欠損マウスにおける炎症性腸疾患と網羅的エピゲノム解析 (2020)
  • Author(s): 大迫 誠一郎, 鈴木 壮登, 矢田 健太郎, 上羽 悟史, 陳 旻岑, 荻原 春, 松島 綱治, 市原 学
  • Organizer: 第47回日本毒性学会学術年会
• [Presentation] Neurite retraction in bioaminergic neurons induced by exposure to conditioned medium from acrylamide-treated microglial cells (2020)
  • Author(s): Harue Sato, Cai Zong, Sho Iwama, Benoit Schneider, Makoto Urushitani, Gaku Ichihara
  • Organizer: 第43回日本神経科学大会
• [Presentation] アクリルアミド曝露により誘導されるモノアミン神経変性のメカニズムに関する研究 (2020)
  • Author(s): 佐藤 春恵，宗 才，岩間 聖，Benoit Schneider，漆谷 真，市原 学
  • Organizer: フォーラム2020：衛生薬学・環境トキシコロジー
• [Presentation] Fe3O4-PEG-PLGA経気道曝露による野生型およびNrf2欠損マウスの肺への影響 (2020)
  • Author(s): 佐藤春恵、宗才、市原佐保子、伊東健、山本雅之、Sonja Boland、Armelle Baeza、市原学
  • Organizer: 第15回ナノ・バイオメディカル学会大会
• [Presentation] Pulmonary effect of exposure to Fe3O4-PEG-PLGA nanoparticles via pharyngeal aspiration in wild type and Nrf2 knockout mice (2020)
  • Author(s): Harue Sato, Cai Zong, Sahoko Ichihara, Ken Itoh, Masayuki Yamamoto, Sonja Boland, Armelle Baeza-Squiban, Gaku Ichihara
  • Organizer: 9th NANO Conference
  • Int'l Joint Research
• [Presentation] Role of inflammation in neurodegeneration and carcinogenesis (2020)
  • Author(s): Gaku Ichihara, Cai Zong, Chand Basha, Kouji Matsushima and Seiichiroh Ohsako
  • Organizer: 7th International Postgraduate Conference on Pharmacerutical Sciences(iPoPS 2020)
  • Int'l Joint Research / Invited
• [Presentation] Deletion of Nrf2 enhances susceptibility to neurotoxic effects of acrylamide in mice (2020)
  • Author(s): Frederick Adams Ekuban, Zong Cai, Madoka Takikawa, Kouta Morikawa, Toshihiro Sakurai, Sahoko Ichihara, Ken Itoh, Masayuki Yamamoto, Seiichiro Ohsako, Gaku Ichihara
  • Organizer: 7th International Postgraduate Conference on Pharmacerutical Sciences(iPoPS 2020)
  • Int'l Joint Research
• [Presentation] Alkaline conditions enhance 1,2-dichiloropropane-induced γ-H2AX expression in human cholangiocytes (2020)
  • Author(s): Yusuke Kimura, Cai Zong and Gaku Ichihara
  • Organizer: 7th International Postgraduate Conference on Pharmacerutical Sciences(iPoPS 2020)
  • Int'l Joint Research
• [Presentation] Role of reactive oxygen species in cholangiocarcinoma induced by exposuer to 1,2-Dichloropropane in humans (2020)
  • Author(s): Abigail Ekuban, Cai Zong, Yusuke Kimura, Sahoko Ichihara, Gaku Ichihara
  • Organizer: 7th International Postgraduate Conference on Pharmacerutical Sciences(iPoPS 2020)
  • Int'l Joint Research
• [Presentation] Neurite degeneration in 1C11NE noradrenergic neurons after exposure to culture supernatant of acrylamide treated BV2 microglia (2020)
  • Author(s): Cai Zong, Harue Sato, Sho Iwama, Benoit Schneider, Makoto Urushitani, Gaku Ichihara
  • Organizer: 7th International Postgraduate Conference on Pharmacerutical Sciences(iPoPS 2020)
  • Int'l Joint Research
• [Presentation] Effects of fipronil exposure on BV2 microglia (2020)
  • Author(s): Yuri Ando, Cai Zong, Akira Sato, Makoto Urushitani, Gaku Ichihara
  • Organizer: 7th International Postgraduate Conference on Pharmacerutical Sciences(iPoPS 2020)
  • Int'l Joint Research
• [Presentation] Comparatives study on susceptibility to acrylamide exposure in CATH.a neurons and HepG2 hepatocytes (2020)
  • Author(s): Aina Suzuki, Cai Zong, Gaku Ichihara
  • Organizer: 7th International Postgraduate Conference on Pharmacerutical Sciences(iPoPS 2020)
  • Int'l Joint Research
• [Presentation] Role of reactive oxygen species in cholangiocarcinoma induced by exposure to 1,2-Dichloropropane in humans (2020)
  • Author(s): Abigail Ekuban, Cai Zong, Yusuke Kimura, Sahoko Ichihara, Seiichiroh Ohsako, Gaku Ichihara
  • Organizer: 第19回分子予防環境医学研究会大会
• [Presentation] アルカリ性条件下1,2-ジクロロプロパンはヒト胆管細胞にγH2AX を誘導する (2020)
  • Author(s): 木村優介、宗才、大迫誠一郎、市原学
  • Organizer: 第19回分子予防環境医学研究会大会
• [Presentation] アクリルアミドの曝露は脳内ノルアドレナリン作動性神経線維を減少させる (2020)
  • Author(s): 長嶋大地、張霊逸、原怜、一瀬宏、山野優子、市原学
  • Organizer: 第19回分子予防環境医学研究会大会
• [Presentation] アクリルアミド曝露によるミクログリアを介したモノアミン神経への影響 (2020)
  • Author(s): 佐藤春恵、宗才、岩間聖、Benoit Schneider、漆谷真、市原学
  • Organizer: 第19回分子予防環境医学研究会大会
• [Presentation] アリールハイドロカーボン受容体欠損マウスで観察された炎症性腸疾患 (2020)
  • Author(s): 大迫誠一郎、鈴木壮登、上羽悟史、陳旻岑、萩原春、松島綱治、市原学
  • Organizer: 第19回分子予防環境医学研究会大会
• [Presentation] Deletion of Nrf2 enhances susceptibility to neurotoxic effects of acrylamide in mice (2020)
  • Author(s): Frederick Adams Ekuban, Cai Zong, Madoka Takikawa, Kouta Morikawa, Toshihiro Sakurai, Sahoko Ichihara, Ken Itoh, Masayuki Yamamoto, Seiichiroh Ohsako, Gaku Ichihara
  • Organizer: 第19回分子予防環境医学研究会大会
• [Presentation] Deletion of NRF2 Enhances Susceptibility to Neurotoxic Effects of Acrylamide in Mice (2020)
  • Author(s): F. A. Ekuban, C. Zong, M. Takikawa, K. Morikawa, T. Sakurai, S. Ichihara, K. Itoh, M. Yamamoto, S. Ohsako, and G. Ichihara.
  • Organizer: Society of Toxicology 59th Annual Meeting (SOT2020)
  • Int'l Joint Research
• [Presentation] Nrf2の欠損はアクリルアミド神経毒性に対する感受性を増強する (2020)
  • Author(s): エクバンフレデリック アダムス，宗 才，瀧川 円賀，森川 浩太，櫻井 敏博，市原 佐保子，伊藤健，山本雅之，大迫 誠一郎，市原 学
  • Organizer: 第90回日本衛生学会学術総会
• [Presentation] 1,2-DCP暴露により誘導される胆管がん発生のメカニズムにおけるマクロファージを介したDNA二重鎖切断 (2019)
  • Author(s): 滝澤 亮哉、宗 才、木下 和生、櫻井 敏博、市原 佐保子、市原 学
  • Organizer: 新学術領域「予防を科学する炎症細胞社会学」班会議/ワークショップ
• [Presentation] 1-Bromopropane暴露ラットの慢性・急性期炎症におけるトランスクリプトームパターンの相違 (2019)
  • Author(s): 陳 影、藤渕 航、市原 学、大迫 誠一郎
  • Organizer: 新学術領域「予防を科学する炎症細胞社会学」班会議/ワークショップ
• [Presentation] 1,2-DCP曝露による胆管がん誘導メカニズムにおけるマクロファージを介したDNA二重鎖切断 (2019)
  • Author(s): 滝澤 亮哉, 宗 才, 木下 和生, 櫻井 敏博, 市原 佐保子, 市原 学
  • Organizer: 第46回日本毒性学会学術年会
• [Presentation] ニューロンとミクログリアにおけるアクリルアミドの細胞毒性とオートファジーの関係 (2019)
  • Author(s): 宗 才, 岩間 聖, 山村 征寛, 漆谷 真, 櫻井 敏博, 市原 佐保子, 大迫 誠一郎, 市原 学
  • Organizer: 第46回日本毒性学会学術年会
• [Presentation] Deletion of Nrf2 enhances susceptibility to neurotoxic effects of acrylamide in mice (2019)
  • Author(s): Frederick Adams EKUBAN, Cai ZONG, Kouta MORIKAWA, Madoka TAKIKAWA, Toshihiro SAKURAI, Sahoko ICHIHARA, Seiichiro OHSAKO, Gaku ICHIHARA
  • Organizer: 第46回日本毒性学会学術年会
• [Presentation] Nrf2の欠損はアクリルアミド神経毒性に対する感受性を増強する (2019)
  • Author(s): エクバンフレデリック アダムス，宗 才，森川 浩太，瀧川 円賀，櫻井 敏博，市原 佐保子，大迫 誠一郎，市原 学
  • Organizer: フォーラム2019 ～衛生薬学・環境トキシコロジー
• [Presentation] フィプロニル曝露が引き起こす中枢神経ミクログリアへの影響 (2019)
  • Author(s): 安藤 宥理，宗 才，漆谷 真，市原 学
  • Organizer: フォーラム2019 ～衛生薬学・環境トキシコロジー
• [Presentation] Deletion of Nrf2 enhances susceptibility to neurotoxic effects of acrylamide in mice (2019)
  • Author(s): Frederick Adams EKUBAN, Cai ZONG, Kouta MORIKAWA, Madoka TAKIKAWA, Toshihiro SAKURAI, Sahoko ICHIHARA, Seiichiro OHSAKO, Gaku ICHIHARA
  • Organizer: The Environmental Response V, 17th JBS Biofrontier Symposium
  • Int'l Joint Research
• [Presentation] 殺虫剤フィプロニルの室内散布後に神経症状を呈した一例 (2019)
  • Author(s): 市原 学
  • Organizer: 第47回産業中毒・生物学的モニタリング研究会
• [Presentation] Deletion of Nrf2 enhances susceptibility to neurotoxic effects of actylamide in mice (2019)
  • Author(s): Frederick Ekuban, Zong Cai, Kouta Morikawa, Madoka Takikawa, Toshihiro Sakurai, Sahoko Ichihara, Ken Itoh, Masayuki Yamamoto, Seiichiroh Ohsako, Gaku Ichihara
  • Organizer: 第14回ナノ・バイオメディカル学会
• [Presentation] Inflammatoty bowel desease observed in aryl-hydrocarbon receptor gene knock-out mice (2019)
  • Author(s): Souto SUZUKI, Satochi UEHA, Ming-Chen CHEN, Haru OGIWARA, Koji MATSUSHIMA, Gaku ICHIHARA, Seiichiroh OHSAKO
  • Organizer: 1st International Symposium on Inflammation Cellular Sociology
  • Int'l Joint Research
• [Presentation] Deletion of Nrf2 enhances susceptibility to neurotoxic effects of actylamide in mice (2019)
  • Author(s): Frederick Ekuban, Zong Cai, Kouta Morikawa, Madoka Takikawa, Toshihiro Sakurai, Sahoko Ichihara, Ken Itoh, Masayuki Yamamoto, Seiichiroh Ohsako, Gaku Ichihara
  • Organizer: 1st International Symposium on Inflammation Cellular Sociology
  • Int'l Joint Research
• [Book] 産業保健マニュアル (2021)
  • Author(s): 森 晃爾
  • Total Pages: 488
  • Publisher: 南山堂
  • ISBN: 9784525184582
• [Remarks] 非アポトーシスカスパーゼが環境起因性中枢神経障害のメカニズムに果たす役割
  • URL: https://www.rs.tus.ac.jp/oeh/theme/theme5.html