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Reconstruction of Past Fish Communities Using eDNA Metabarcoding in Coastal Sediment Cores

Research Project

Project/Area Number 19H04284
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 63040:Environmental impact assessment-related
Research InstitutionUniversity of Hyogo

Principal Investigator

Doi Hideyuki  兵庫県立大学, 情報科学研究科, 准教授 (80608505)

Co-Investigator(Kenkyū-buntansha) 加 三千宣  愛媛大学, 沿岸環境科学研究センター, 准教授 (70448380)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords環境DNA / 堆積物 / 海洋 / 魚類 / メタバーコーディング / 近過去復元 / 哺乳類 / 環境影響調査
Outline of Research at the Start

堆積物コア試料を用いた近過去の生態系状態の復元は、過去の生態系の状態について把握することができる数少ない手法である。しかし、これまで手法の制約から復元できる生物群集は、プランクトンなどの低次消費者に限られており、魚類群集については、その群集を復元することができなかった。近年急速に発展してきた、環境水中からDNAを取り出して、多種の同時検出を可能とする環境DNAメタバーコーディング技術は、これまで復元できなかった堆積物試料からの魚類群集の解明に大いに威力を発揮する可能性がある。本研究では、堆積物試料に環境DNAメタバーコーディングを適用することにより近過去の魚類群集を再現する。

Outline of Final Research Achievements

Paleoceanographic studies on fish have been limited to the presence of scaled rock lobsters in sediments, and there has been no way to reconstruct other fish communities as a whole. Our project focuses on environmental DNA (eDNA0 remaining in sediment cores. The eDNA refers to DNA fragments from various organisms contained in the environment, and by collecting and analyzing these fragments, it will be possible to determine what species live in the environment. We collected sediment core samples from the deepest part of Beppu Bay and conducted eDNA analysis. We conducted the eDNA metabarcoding using MIFish universal primers which comprehensively amplify fish DNA. Then, we confirmed that these fish species can be detected in the recent-past layers of the sediment cores. We demonstrated that metabarcoding is possible for sediment samples using MIFish universal primers.

Academic Significance and Societal Importance of the Research Achievements

本研究により、環境DNAを用いて、海洋における過去数百年の魚類群集の変動について、堆積物コア試料による近過去復元が可能であることを明らかにした。これは今後の水産資源管理、生態系管理において、生態系変動予測に利用できる データを得ることができる有力な手法となったと考えられる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (20 results)

All 2022 2021 2020 2019

All Journal Article (16 results) (of which Peer Reviewed: 16 results,  Open Access: 11 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Reconstruction of 100-year dynamics in Daphnia spawning activity revealed by sedimentary DNA.2022

    • Author(s)
      Narumi Tsugeki, Kai Nakane, Hideyuki Doi, Natsuki Ochi and Michinobu Kuwae
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 1741-1741

    • DOI

      10.1038/s41598-021-03899-0

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Causal analysis of the temperature impact on deep-sea biodiversity.2021

    • Author(s)
      Hideyuki Doi , Moriaki Yasuhara and Masayuki Ushio
    • Journal Title

      Biology Letters

      Volume: 17 Issue: 7 Pages: 20200666-20200666

    • DOI

      10.1098/rsbl.2020.0666

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of biodiversity metrics by environmental DNA metabarcoding compared with visual and capture surveys of river fish communities2021

    • Author(s)
      Doi Hideyuki、Inui Ryutei、Matsuoka Shunsuke、Akamatsu Yoshihisa、Goto Masuji、Kono Takanori
    • Journal Title

      Freshwater Biology

      Volume: ‐ Issue: 7 Pages: 1257-1266

    • DOI

      10.1111/fwb.13714

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Taxonomic, functional, and phylogenetic diversity of fungi in a forest-tundra ecotone in Qu?bec2021

    • Author(s)
      Matsuoka Shunsuke、Doi Hideyuki、Masumoto Shota、Kitagawa Ryo、Nishizawa Keita、Tanaka Kaho、Hasegawa Motohiro、Hobara Satoru、Osono Takashi、Mori Akira S.、Uchida Masaki
    • Journal Title

      Polar Science

      Volume: 27 Pages: 100594-100594

    • DOI

      10.1016/j.polar.2020.100594

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simultaneous absolute quantification and sequencing of fish environmental DNA in a mesocosm by quantitative sequencing technique.2021

    • Author(s)
      Hoshino T, Nakao R, Doi H, Minamoto T
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 4372-4372

    • DOI

      10.1038/s41598-021-83318-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Suppression of environmental DNA degradation in water samples associated with different storage temperature and period using benzalkonium chloride2020

    • Author(s)
      Takahara Teruhiko、Taguchi Junya、Yamagishi Satoshi、Doi Hideyuki、Ogata Shigeki、Yamanaka Hiroki、Minamoto Toshifumi
    • Journal Title

      Limnology and Oceanography: Methods

      Volume: 18 Issue: 8 Pages: 437-445

    • DOI

      10.1002/lom3.10374

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sedimentary DNA tracks decadal-centennial changes in fish abundance2020

    • Author(s)
      Kuwae Michinobu、Tamai Hiromichi、Doi Hideyuki、Sakata Masayuki K.、Minamoto Toshifumi、Suzuki Yoshiaki
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s42003-020-01282-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Broad-scale detection of environmental DNA for an invasive macrophyte and the relationship between DNA concentration and coverage in rivers2020

    • Author(s)
      Doi Hideyuki、Akamatsu Yoshihisa、Goto Masuji、Inui Ryutei、Komuro Takashi、Nagano Mariko、Minamoto Toshifumi
    • Journal Title

      Biological Invasions

      Volume: 23 Issue: 2 Pages: 507-520

    • DOI

      10.1007/s10530-020-02380-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exploring a legendary giant squid: An environmental DNA approach.2020

    • Author(s)
      Wada T, Doi H, Togaki D, Kaida R, Nagano M, Katano I, Suzuki M, Ohtani T, Mitsuhashi H.
    • Journal Title

      Marine Biology

      Volume: 167 Issue: 11 Pages: 1-7

    • DOI

      10.1007/s00227-020-03773-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Environmental DNA metabarcoding for biodiversity monitoring of a highly diverse tropical fish community in a coral reef lagoon: Estimation of species richness and detection of habitat segregation2020

    • Author(s)
      Oka Shin‐ichiro、Doi Hideyuki、Miyamoto Kei、Hanahara Nozomi、Sado Tetsuya、Miya Masaki
    • Journal Title

      Environmental DNA

      Volume: 3 Issue: 1 Pages: 55-69

    • DOI

      10.1002/edn3.132

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols2020

    • Author(s)
      Minamoto Toshifumi、Miya Masaki、Sado Tetsuya、Seino Satoquo、Doi Hideyuki、Kondoh Michio、Nakamura Keigo、Takahara Teruhiko、Yamamoto Satoshi、Yamanaka Hiroki、Araki Hitoshi、Iwasaki Wataru、Kasai Akihide、Masuda Reiji、Uchii Kimiko
    • Journal Title

      Environmental DNA

      Volume: 3 Issue: 1 Pages: 8-13

    • DOI

      10.1002/edn3.121

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The detection of aquatic macroorganisms using environmental DNA analysis?A review of methods for collection, extraction, and detection2019

    • Author(s)
      Tsuji Satsuki、Takahara Teruhiko、Doi Hideyuki、Shibata Naoki、Yamanaka Hiroki
    • Journal Title

      Environmental DNA

      Volume: 1 Issue: 2 Pages: 99-108

    • DOI

      10.1002/edn3.21

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatial structures of fungal DNA assemblages revealed with eDNA metabarcoding in a forest river network in western Japan.2019

    • Author(s)
      Matsuoka S, Sugiyama Y, Sato H, Katano I, Harada K, Doi H.
    • Journal Title

      Metabarcoding and Metagenomics

      Volume: 3

    • DOI

      10.3897/mbmg.3.36335

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Seasonal change in environmental DNA concentration of a submerged aquatic plant species2019

    • Author(s)
      Matsuhashi Saeko、Minamoto Toshifumi、Doi Hideyuki
    • Journal Title

      Freshwater Science

      Volume: 38 Issue: 3 Pages: 654-660

    • DOI

      10.1086/704996

    • NAID

      120006996926

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimating native and invasive crayfish distributions in relation to culvert barriers with environmental DNA2019

    • Author(s)
      Ikeda Kousuke、Doi Hideyuki、Terui Shigeharu、Kato Atsuko、Mitsuzuka Takashi、Kawai Tadashi、Negishi Junjiro N.
    • Journal Title

      Freshwater Science

      Volume: 38 Issue: 3 Pages: 629-641

    • DOI

      10.1086/704998

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA.2019

    • Author(s)
      Uchii K, Doi H, Okahashi T, Katano I, Yamanaka H, Sakata KM, Minamoto T.
    • Journal Title

      Environmental DNA

      Volume: 1 Issue: 4 Pages: 359-367

    • DOI

      10.1002/edn3.37

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 環境中に漂うDNAの情報から何がわかってきたか?2021

    • Author(s)
      土居秀幸
    • Organizer
      第64回日本生態学会 公開講演会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Environmental DNA methods for macroorganism populations2021

    • Author(s)
      Hideyuki Doi
    • Organizer
      The 1st DNAqua-Net international conference
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 海底堆積物からの環境DNA検出による 近過去生物群集の再現2019

    • Author(s)
      海田竜太郎, 土居秀,永野真理子,加三千宣,玉井大道,倉田純
    • Organizer
      第41回日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Book] 環境DNA2021

    • Author(s)
      一般社団法人 環境DNA学会、土居 秀幸、近藤 倫生
    • Total Pages
      300
    • Publisher
      共立出版
    • ISBN
      9784320058163
    • Related Report
      2020 Annual Research Report

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Published: 2019-04-18   Modified: 2023-01-30  

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