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Establishment of a biological method to treat 1,4-dioxane in landfill leachate by selective enrichment and activation of indigenous 1,4-dioxane-degrading bacteria

Research Project

Project/Area Number 19H04301
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 64020:Environmental load reduction and remediation-related
Research InstitutionOsaka University

Principal Investigator

Inoue Daisuke  大阪大学, 大学院工学研究科, 准教授 (70448091)

Co-Investigator(Kenkyū-buntansha) 池 道彦  大阪大学, 大学院工学研究科, 教授 (40222856)
矢吹 芳教  地方独立行政法人大阪府立環境農林水産総合研究所(環境研究部、食と農の研究部及び水産研究部), その他部局等, 主任研究員 (00360818)
黒田 真史  大阪大学, 工学研究科, 助教 (20511786)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2022: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2021: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords1,4-ジオキサン / 処分場浸出水 / 生物学的処理 / 分解菌集積 / 処分場進出水 / 選択的分解菌集積法 / 安定的活性化法
Outline of Research at the Start

1,4-ジオキサンは、水環境中における残留性が極めて高く、発がん性が疑われている重要な水環境汚染物質である。本研究では、最終処分場において広く適用できる生物学的1,4-ジオキサン処理技術を確立することを目標とし、流動担体と特異的増殖基質を用いた土着1,4-ジオキサン分解菌の迅速かつ選択的な集積法と、集積した分解菌の安定した活性発現のための好適な運転制御ならびに分解阻害物質の前処理法を確立し、土着分解菌を活用した現場での処理の可能性を明らかにすることを目指す。

Outline of Final Research Achievements

This study aimed to establish a method to rapidly and selectively enrich indigenous 1,4-dioxane-degrading bacteria in the actual landfill leachate treatment facility, and to demonstrate efficient biological 1,4-dioxane treatment using the enriched 1,4-dioxane-degrading bacteria. Lab-scale experiments revealed that 1,4-dioxane-degrading bacteria present in the landfill leachate treatment facility can be enriched onto the carrier material, and 1,4-dioxane can be efficiently degraded under various environmental and operating conditions by selecting an appropriate carrier material. Furthermore, a demonstration test in an actual landfill leachate treatment facility clarified that 1,4-dioxane-degrading bacteria can be enriched by the introduction of carrier materials in situ, and resultantly 1,4-dioxane treatment capability can be enhanced for a long period.

Academic Significance and Societal Importance of the Research Achievements

本研究では、最終処分場の浸出水に含まれる1,4-ジオキサンの効率的な処理を目指し、既設の水処理施設に生息する土着の分解菌を活用する新規の生物学的1,4-ジオキサン処理技術を確立した。本研究で確立した処理技術は、大規模改修や高度処理の導入が望めない小規模な最終処分場の簡易水処理施設でも適用可能であり、多くの最終処分場における1,4-ジオキサン処理に活用され、1,4-ジオキサンに起因するリスクの低減に貢献し得るものと期待される。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (21 results)

All 2023 2022 2021 2020 2019

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (17 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] Development of a New Polar Organic Chemical Integrative Sampler for 1,4‐dioxane Using Silicone Membrane as a Diffusion Barrier2023

    • Author(s)
      Noro Kazushi、Endo Satoshi、Inoue Daisuke、Suzuki Natsumi、Kameoka Hiroshi、Ono Junko、Nakamura Satoshi、Yabuki Yoshinori
    • Journal Title

      Environmental Toxicology and Chemistry

      Volume: 42 Issue: 2 Pages: 296-302

    • DOI

      10.1002/etc.5518

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of tetrahydrofuran at enhancing the 1,4-dioxane degradation ability of activated sludge lacking prior exposure to 1,4-dioxane2022

    • Author(s)
      Inoue Daisuke、Hisada Kazuki、Ike Michihiko
    • Journal Title

      Water Science and Technology

      Volume: 86 Issue: 7 Pages: 1707-1718

    • DOI

      10.2166/wst.2022.296

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Treatment of 1,4-dioxane-containing water using carriers immobilized with indigenous microorganisms in landfill leachate treatment sludge: A laboratory-scale reactor study2021

    • Author(s)
      Daisuke Inoue, Takumi Yoshikawa, Takuya Okumura, Yoshinori Yabuki, Michihiko Ike
    • Journal Title

      Journal of Hazardous Materials

      Volume: 414 Pages: 125497-125497

    • DOI

      10.1016/j.jhazmat.2021.125497

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Carbon sources that enable enrichment of 1,4-dioxane-degrading bacteria in landfill leachate2020

    • Author(s)
      Inoue Daisuke、Hisada Kazuki、Okumura Takuya、Yabuki Yoshinori、Yoshida Gen、Kuroda Masashi、Ike Michihiko
    • Journal Title

      Biodegradation

      Volume: 31 Issue: 1-2 Pages: 23-34

    • DOI

      10.1007/s10532-019-09891-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] Pseudonocardia sp. D17による塩素化エチレン類の好気分解機構の解明2023

    • Author(s)
      西峯隆悟、南薗洸佑、井上大介、池道彦
    • Organizer
      第57回日本水環境学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 塩素化エチレン類複合汚染に対するPseudonocardia sp. D17の適用の検討2023

    • Author(s)
      藤原進平、西峯隆悟、南薗洸佑、井上大介、池道彦
    • Organizer
      第57回日本水環境学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Aerobic degradation of chlorinated ethenes without auxiliary substrates by Pseudonocardia sp. D172022

    • Author(s)
      Ryugo Nishimine, Kosuke Minamizono, Masatoshi Nakazawa, Daisuke Inoue, Michihiko Ike
    • Organizer
      Water and Environment Technology Conference Online 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pseudonocardia sp. D17による塩素化エチレン類の好気分解機構の検討2022

    • Author(s)
      西峯隆悟、南薗洸佑、中澤昌俊、井上大介、池道彦
    • Organizer
      日本水処理生物学会第58回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Aerobic degradation of chloroethylenes by Pseudonocardia sp. D172021

    • Author(s)
      Kosuke Minamizono, Daisuke Inoue, Michihiko Ike
    • Organizer
      The 13th Joint Workshop on Advanced Engineering Technology for Environment and Energy
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Treatment of 1,4-dioxane-containing water by bioaugmentation of Pseudonocardia sp. D17 with fluidized carrier materials2021

    • Author(s)
      Daisuke Inoue, Takumi Yoshikawa, Michihiko Ike
    • Organizer
      The 14th Annual Conference on the Challenges in Environmental Science and Engineering (CESE-2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pseudonocardia sp. D17株による塩素化エチレン類の好気分解能の評価2021

    • Author(s)
      南薗洸佑, 西峯隆悟, 中澤昌俊, 井上大介, 池道彦
    • Organizer
      日本水処理生物学会第57回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Pseudonocardia sp. D17が保有する1,4-ジオキサン分解酵素による塩素化エチレン類の好気的分解2021

    • Author(s)
      西峯隆悟, 南薗洸佑, 中澤昌俊, 井上大介, 池道彦
    • Organizer
      第73回日本生物工学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 分解菌付着担体を用いた1,4-ジオキサン含有浸出水の処理に関する検討2021

    • Author(s)
      好川拓実, 井上大介, 池道彦
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Pseudonocardia sp. D17による塩素化エチレン類の好気的代謝機能の評価2021

    • Author(s)
      西峯隆吾, 南薗洸佑, 中澤昌俊, 井上大介, 池道彦
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 浸出水中の1,4-ジオキサンの微生物処理の長期変動2020

    • Author(s)
      矢吹芳教, 小野純子, 吉田弦, 井上大介, 好川拓実
    • Organizer
      第23回日本水環境学会シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 分解菌のバイオオーグメンテーションによる1,4-ジオキサン処理技術の検討2020

    • Author(s)
      好川拓実, 井上大介, 池道彦
    • Organizer
      廃棄物資源循環学会第31回研究発表会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 1,4-ジオキサン分解菌Pseudonocardia sp. D17株によるクロロエチレン類の好気的分解2020

    • Author(s)
      南薗洸佑, 井上大介, 池道彦
    • Organizer
      2020年度日本生物工学会関西支部学生オンライン発表会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 微生物付着担体を用いた最終処分場浸出水中の1,4-ジオキサン処理に関する基礎的検討2020

    • Author(s)
      井上大介、好川拓実、奥村拓也、矢吹芳教、池道彦
    • Organizer
      第54回日本水環境学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Biological treatment of 1,4-dioxane by fluidized bed reactors with bacterial communities enriched on different types of carriers2019

    • Author(s)
      Takumi Yoshikawa, Takuya Okumura, Daisuke Inoue, Yoshinori Yabuki, Michihiko Ike
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 1,4-ジオキサン分解菌による多様な環状エーテル類の分解能の評価2019

    • Author(s)
      中澤昌俊、井上大介、黒田真史、池道彦
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Enrichment of 1,4-dioxane-degrading bacteria in activated sludge using tetrahydrofuran2019

    • Author(s)
      Daisuke Inoue, Kazuki Hisada, Masashi Kuroda, Michihiko Ike
    • Organizer
      8th IWA Microbial Ecology and Water Engineering Specialist Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2024-01-30  

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