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Microbial-mineral interactions of toxic metals for stabilization of mine waste

Research Project

Project/Area Number 19H04302
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 64020:Environmental load reduction and remediation-related
Research InstitutionKyushu University

Principal Investigator

Hammaura Natsuko  九州大学, 理学研究院, 教授 (50554466)

Co-Investigator(Kenkyū-buntansha) 光延 聖  愛媛大学, 農学研究科, 准教授 (70537951)
鹿島 裕之  国立研究開発法人海洋研究開発機構, 超先鋭研究開発部門(超先鋭研究開発プログラム), 研究員 (70780914)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2022: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords微生物地球化学 / 微生物環境浄化 / 微生物金属代謝 / 微生物重金属代謝 / ヒ素代謝 / 重金属汚染 / アンチモン還元 / ヒ素酸化 / ヒ素還元 / 微生物ヒ素代謝 / 地球微生物学 / ヒ素酸化還元 / アンチモン / 金属生物変換
Outline of Research at the Start

猛毒のヒ素などの有害金属は世界で最も被害の深刻な汚染物質の一つであり、鉱山開発など人為的な汚染源や、鉱物や採掘ずりからの溶出など自然汚染源からも環境に放出され、長期的な安定化が望まれている。本研究では、有害金属動態プロセスのモデル化と安定化技術への応用を目指し、鉱山廃棄物の有害金属挙動に影響する微生物-鉱物相互作用を解明する。特に、深刻な健康被害を引き起こすとして重要汚染物質に指定されているヒ素とアンチモンに着目し、鉱山廃棄物の有害金属動態プロセスに微生物作用を組み込んだ包括的モデルを構築し、固定化に寄与する新規微生物機構を同定するとともに、長期的安定性の評価を実施する。

Outline of Final Research Achievements

The objective of this study was to elucidate the microbial-mineral interactions that affect the stability and geochemical cycling of toxic metal(loid)s in contaminated environments. To investigate the diversity of microorganisms involved in the transformation of toxic metal(loid)s, arsenic- and antimony-metabolizing microorganisms were enriched and isolated from contaminated environmental samples. Phylogenetically diverse metal(loid)-transforming bacteria were obtained and their biotransformation mechanisms were characterized by using genomic approach. The metal metabolism mechanism of these novel microorganisms is expected to contribute to the recovery of useful metals by producing rare metal crystals and to the stabilization of Sb through the reduction of iron oxide minerals.

Academic Significance and Societal Importance of the Research Achievements

固体の有害金属と微生物の相互作用は、廃棄物からの長期的な拡散や二次汚染に関与しており、鉱山廃棄物の安定性評価や汚染対策を講じる上でも、そのプロセスの解明が必要とされている。本研究では、新規汚染物質であるアンチモンの環境動態に影響を及ぼす多様な微生物機構を明らかにした。この成果により、有害金属の固定化・安定化に寄与する微生物機構が明らかになり、微生物を利用した環境浄化技術の発展に寄与できる。また、本研究で得られた微生物金属代謝機構を活用し、有用金属の回収やバイオミネラルの新規ナノマテリアル応用など、資源の有効利用と環境浄化の両立を図る技術としての貢献も期待できる。

Report

(3 results)
  • 2023 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (12 results)

All 2021 2019

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 5 results,  Invited: 2 results)

  • [Journal Article] Draft genome analysis of Cupriavidus sp. strain IK-TO18, isolated from an antimony-contaminated sediment2021

    • Author(s)
      Okubo T., Nakajima N., Yamamura S., Hamamura N.
    • Journal Title

      Microbiology Resource Announcements

      Volume: 10 Issue: 38

    • DOI

      10.1128/mra.00724-21

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Draft genome sequence of an anaerobic arsenite-oxidizing Halomonas sp. strain ANAO-440 isolated from an alkaline saline lake in Khovsgol, Mongolia2021

    • Author(s)
      Hamamura N., Damdinsuren, N., Nakajima N., Yamamura, S.
    • Journal Title

      Microbiology Resource Announcements

      Volume: 10 Issue: 42

    • DOI

      10.1128/mra.00899-21

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Microbe-mineral interaction and biotransformation of toxic metalloids2021

    • Author(s)
      Hamamura N, Yamashita D, Mitsunobu S.
    • Organizer
      International Conference on Materials and Systems for Sustainability 2021
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Biotransformation potential of antimony-reducing microbial consortium obtained from stibnite mine tailing soil2021

    • Author(s)
      Yamashita D, Mitsunobu S, Hamamura N.
    • Organizer
      International Conference on Materials and Systems for Sustainability 2021
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of microbial communities associated with microbial fuel cells2021

    • Author(s)
      Yoshimura Y, Okubo T, Kashima H, Hamamura N.
    • Organizer
      International Conference on Materials and Systems for Sustainability 2021
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biotransformation potential of antimony-reducing microbial consortium obtained from stibnite mine tailing soil2021

    • Author(s)
      Yamashita D, Mitsunobu S, Hamamura N.
    • Organizer
      日本微生物生態学会第34回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒ素・アンチモン複合汚染環境における微生物群集構造および金属代謝機能の解析2021

    • Author(s)
      吉村雄弥, 鹿島裕之, 光延聖, 濱村奈津子
    • Organizer
      日本微生物生態学会第34回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Diversity of microbial arsenic and antimony transformation pathways associated with antimony mine tailing2019

    • Author(s)
      Hamamura, N., Yamashita, Y., Kataoka, T., Mitsunobu, S.
    • Organizer
      Goldschmidt2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微生物有害元素代謝の多様性:嫌気的アンチモン酸化細菌群の代謝機構と系統分布2019

    • Author(s)
      大久保公貴、山下葉里子、濱村奈津子
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 土壌の複雑な微環境における土壌細菌群集構造のミクロレベル解析2019

    • Author(s)
      高山清一郎、和穎朗太、荒井見和、濱村奈津子
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヒ素の環境挙動に影響を及ぼす微生物代謝機構の多様性2019

    • Author(s)
      濱村奈津子
    • Organizer
      資源・素材学会2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Microbial Biotransformation of Toxic Metalloids and Its Bioremediation Potentials2019

    • Author(s)
      Hamamura, N., Okubo, T.,Mitsunobu, S.
    • Organizer
      International Conference on Materials and Systems for Sustainability 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2025-01-30  

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