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Development of the novel methanol bioconversion systems for a low-carbon and resource-recycling society and elucidation of their molecular basis

Research Project

Project/Area Number 19H04326
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 64050:Sound material-cycle social systems-related
Research InstitutionKyoto University

Principal Investigator

YURIMOTO Hiroya  京都大学, 農学研究科, 准教授 (00283648)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywords遺伝子発現制御 / 転写因子 / シグナル伝達 / 異種タンパク質生産 / メタン / メタノール / メタノール酵母 / メタノール誘導性 / メタノール誘導 / メタノール変換 / 天然ガス / バイオマス / 環境技術 / 応用微生物 / バイオテクノロジー / 発現制御 / 生体機能利用
Outline of Research at the Start

メタノールを低炭素・資源循環型物質生産体系の基幹物質として利用する工業体系「メタノールエコノミー」が提唱されている。本研究では、メタンやメタノールなどのC1化合物を利用するC1微生物を、メタノールエコノミーにおけるバイオプロセスにおいて高度活用することを目的とし、C1微生物の代謝生理機能の分子基盤を解明するとともに、「メタノールから有用物質への変換プロセス」と「様々な炭素資源からメタノールへの変換プロセス」に関する新技術開発を行う。

Outline of Final Research Achievements

For the purpose of establishment of the low-carbon and circular society, “methanol economy”, in which methanol is used as a key compound for the production of useful compounds, is advocated. In this project, we investigated molecular basis of C1-metabolism in C1-microorganisms which can utilize methane or methanol. We elucidated physiological functions of transcriptional regulators and molecular mechanism of methanol-dependent gene regulation in methylotrophic yeasts which are used for heterologous protein production. Furthermore, we developed the basic technology of methanol production from various carbon resources such as methane and biomass and production of useful compounds from methanol.

Academic Significance and Societal Importance of the Research Achievements

メタノール酵母によるメタノールからの有用タンパク質生産の根幹を担うメタノール誘導制御機構について、転写制御因子の生理機能やメタノール濃度に応答した遺伝子発現制御機構を明らかにした。また、メタンやバイオマスからのメタノール変換プロセスおよびメタノールから有用物質への変換プロセスの新技術開発に活用できる基盤的知見を得た。本研究に明らかとなったC1微生物代謝生理機能の分子基盤や有用物質生産のための基盤技術は、低炭素循環型社会におけるバイオプロセスで活用することができる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Products Report
  • Research Products

    (23 results)

All 2023 2022 2021 2020 2019 Other

All Journal Article (12 results) (of which Peer Reviewed: 11 results,  Open Access: 3 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 5 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Regulation of Peroxisome Homeostasis by Post-Translational Modification in the Methylotrophic Yeast Komagataella phaffii2022

    • Author(s)
      Ohsawa Shin、Oku Masahide、Yurimoto Hiroya、Sakai Yasuyoshi
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 10 Pages: 887806-887806

    • DOI

      10.3389/fcell.2022.887806

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Technologies for heterologous protein production by methylotrophic yeasts using various carbon sources other than methanol2021

    • Author(s)
      髙木 忍、由里本 博也、阪井 康能
    • Journal Title

      Seibutsu-kogaku Kaishi

      Volume: 99 Issue: 10 Pages: 516-520

    • DOI

      10.34565/seibutsukogaku.99.10_516

    • NAID

      130008107554

    • ISSN
      0919-3758, 2435-8630
    • Year and Date
      2021-10-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methylotrophic bacterium-based molecular sensor for the detection of low concentrations of methanol2021

    • Author(s)
      Casaroli Viviane Carnier、Orita Izumi、Katayama Shiori、Yurimoto Hiroya、Sakai Yasuyoshi、Fukui Toshiaki
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 132 Issue: 3 Pages: 247-252

    • DOI

      10.1016/j.jbiosc.2021.05.002

    • NAID

      40022687974

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fatty acid composition of the methylotrophic yeast Komagataella phaffii grown under low‐ and high‐methanol conditions2021

    • Author(s)
      Ma Pengli、Takashima Shigeo、Fujita Chikako、Yamada Saya、Oshima Yusuke、Cai Hao‐Liang、Yurimoto Hiroya、Sakai Yasuyoshi、Hayakawa Takashi、Shimada Masaya、Ning Xia、Wei Baoyao、Nakagawa Tomoyuki
    • Journal Title

      Yeast

      Volume: 38 Issue: 10 Pages: 541-548

    • DOI

      10.1002/yea.3655

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methanol sensor Wsc1 and MAP kinase suppress degradation of methanol-induced peroxisomes in methylotrophic yeast2021

    • Author(s)
      Ohsawa Shin、Inoue Koichi、Isoda Takahiro、Oku Masahide、Yurimoto Hiroya、Sakai Yasuyoshi
    • Journal Title

      Journal of Cell Science

      Volume: - Issue: 9

    • DOI

      10.1242/jcs.254714

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methanol bioeconomy: promotion of rice crop yield in paddy fields with microbial cells prepared from natural gas‐derived C1 compound2020

    • Author(s)
      Yurimoto Hiroya、Iguchi Hiroyuki、Di Thien Do Thi、Tani Akio、Okumoto Yutaka、Ota Atsushi、Yamauchi Takahiro、Akashi Takahiro、Sakai Yasuyoshi
    • Journal Title

      Microbial Biotechnology

      Volume: - Issue: 4 Pages: 1385-1396

    • DOI

      10.1111/1751-7915.13725

    • NAID

      120006940304

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methanol production by reversed methylotrophy constructed in Escherichia coli2020

    • Author(s)
      Takeya Tomoyuki、Yamakita Miyabi、Hayashi Daisuke、Fujisawa Kento、Sakai Yasuyoshi、Yurimoto Hiroya
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 84 Issue: 5 Pages: 1062-1068

    • DOI

      10.1080/09168451.2020.1715202

    • NAID

      120006840155

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 合成生物学によるスーパーメタン酸化生体触媒の創製2020

    • Author(s)
      由里本博也、阪井康能
    • Journal Title

      日本エネルギー学会機関誌 えねるみくす

      Volume: 99 Pages: 141-146

    • NAID

      130007824225

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Community composition and methane oxidation activity of methanotrophs associated with duckweeds in a fresh water lake.2019

    • Author(s)
      Iguchi, H., Umeda, R., Taga, H., Oyama, T., Yurimoto, H., Sakai, Y.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: in press Issue: 4 Pages: 450-455

    • DOI

      10.1016/j.jbiosc.2019.04.009

    • NAID

      120006724639

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methylotrophic Yeasts: Current Understanding of Their C1-Metabolism and its Regulation by Sensing Methanol for Survival on Plant Leaves2019

    • Author(s)
      Yurimoto Hiroya、Sakai Yasuyoshi
    • Journal Title

      Current Issues in Molecular Biology

      Volume: 33 Pages: 197-210

    • DOI

      10.21775/cimb.033.197

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expression system2019

    • Author(s)
      Takagi Shinobu、Tsutsumi Noriko、Terui Yuji、Kong XiangYu、Yurimoto Hiroya、Sakai Yasuyoshi
    • Journal Title

      FEMS Yeast Research

      Volume: 19 Issue: 6

    • DOI

      10.1093/femsyr/foz059

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メタノールバイオエコノミー:C1 微生物代謝生理の分子基盤を活用した資源循環型社会の構築2019

    • Author(s)
      由里本博也、阪井康能
    • Journal Title

      酵素工学ニュース

      Volume: 82 Pages: 26-31

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Candida boidiniiにおけるメタノール誘導性転写因子Mpp1の生理機能解析2022

    • Author(s)
      井上紘一、岩瀬公佑、由里本博也、阪井康能
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] プロパン資化性細菌Gordonia sp. TY-5株におけるC1代謝と発現制御2022

    • Author(s)
      木田航平、岩崎光司、由里本博也、阪井康能
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] C1微生物-植物間相互作用による炭素循環と作物増収2022

    • Author(s)
      由里本博也、阪井康能
    • Organizer
      日本農芸化学会2022年度大会シンポジウム「微生物と植物が駆動する新しい物質循環像とバイオ分野への展開」
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] メタノールから生産する微生物製剤の葉面散布による作物増収2021

    • Author(s)
      由里本博也
    • Organizer
      バイオインダストリー協会「C1バイオエコノミー勉強会 -新たな炭素循環像を基にしたC1炭素の循環的利用-」
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] C1酵母で拓くメタノールバイオエコノミー2021

    • Author(s)
      由里本博也
    • Organizer
      日本農芸化学会2021年度大会シンポジウム「酵母研究の産業利用への展開;いま「Non-conventional yeasts」が新しい」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] メタノールバイオエコノミーで活用するC1酵母のメタノール誘導機構2020

    • Author(s)
      由里本博也
    • Organizer
      第198回酵母細胞研究会例会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Regulation of the Expression of a Methanol-Induced Transcription Factor Mpp1 in the Methylotrophic Yeast Candida boidinii2019

    • Author(s)
      Hiroya Yurimoto, Koichi Inoue, and Yasuyoshi Sakai
    • Organizer
      The 35th International Specialized Symposium on Yeasts
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] C1酵母の葉面での生存戦略とメタノール感知機構2019

    • Author(s)
      由里本博也
    • Organizer
      第18回新産業酵母研究会講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] 微生物やその細胞壁成分の葉面散布による酒米の増収に成功

    • URL

      https://www.kyoto-u.ac.jp/ja/research-news/2020-12-11-0

    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] 小胞体内タンパク質フォールディング促進因子2023

    • Inventor(s)
      阪井 康能、由里本 博也、寳関 淳
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2023-203989
    • Filing Date
      2023
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] メタンからの化学品の製造方法2021

    • Inventor(s)
      山本恭士、丸山悟史、阪井康能、由里本博也
    • Industrial Property Rights Holder
      山本恭士、丸山悟史、阪井康能、由里本博也
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2021 Annual Research Report

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Published: 2019-04-18   Modified: 2025-03-27  

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