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Development of near-Infrared fluorescence imaging in the second optical window

Research Project

Project/Area Number 19H04459
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 90110:Biomedical engineering-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

JIN TAKASHI  国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (80206367)

Co-Investigator(Kenkyū-buntansha) 精山 明敏  京都大学, 医学研究科, 教授 (70206605)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords生体の第2光学窓 / 近赤外 / 短波赤外 / 蛍光 / 生体イメージング / 分子イメージング / 乳がん / 生体の第2光学窓 / 生体蛍光イメージング / 非侵襲生体イメージング / インドシアニングリーン / 蛍光イメージング / 深部イメージング / 細胞状態操作 / 蛍光プローブ / がん / 第2光学窓
Outline of Research at the Start

短波赤外領域の光(波長1-1.4um、生体の第2光学窓)は、細胞、組織、臓器、さらには個体レベルで細胞状態を非侵襲で可視化、操作するのに非常に適している。その第一の理由は、可視、近赤外の光に比べて数倍エネルギーが低いため光照射によるダメージが少なく、長時間でのイメージング、光操作が可能なこと。第二の理由は、生体組織による光吸収が少なく透過性に優れており、また組織中での光散乱が少なくなるため、従来法に比べ格段に鮮明な深部蛍光画像が得られることである。本研究では、ヒトでの病態光診断を目指した生体の第2光学窓における個体中での、細胞、組織、臓器の非侵襲ライブイメージング技術の開発をおこなう。

Outline of Final Research Achievements

Light in the shortwave infrared region (wavelength 1-1.4 microns, the second optical window of living organisms) is highly suitable for non-invasive visualization and manipulation of cellular states at the cell, tissue, organ, and even individual level. The first reason is that it is several times lower energy than visible and near-infrared light, which means that light irradiation causes less damage and allows imaging and optical manipulation over a longer period of time. The second reason is that the light absorption by biological tissues is low and the transmittance is excellent, and light scattering in the tissues is reduced, resulting in much clearer deep fluorescence images than with conventional methods. In this study, we developed a "non-invasive imaging technique for cells, tissues, and organs in an individual body at the second optical window" for optical diagnosis of pathological conditions in humans.

Academic Significance and Societal Importance of the Research Achievements

乳がんは15才から60才代女性のがんの罹患率第1位で、その死亡者数は近年増加傾向にあります。大きさ1cm以下の早期乳がん(リンパ節転移なし)は切除手術により、ほぼ確実に治癒できます。そのため乳がんを早期に検出する技術は、乳がん治療において非常に重要です。現在、乳がんの検査法として、マンモグラフィーや超音波診断が用いられていますが、これらの方法では早期乳がんを検出し確定診断することは困難です。本研究は、世界に先駆けて、ヒトでの早期乳がんを短波赤外蛍光で非侵襲イメージングする技術を開発するものであり、乳がんの診断・治療において大きな貢献が期待できます。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (21 results)

All 2022 2021 2020 2019

All Journal Article (16 results) (of which Peer Reviewed: 16 results,  Open Access: 6 results) Book (4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] In Vitro and In Vivo Fluorescence Imaging of Antibody Drug Conjugate-Induced Tumor Apoptosis Using Annexin V-EGFP conjugated Quantum Dots2022

    • Author(s)
      Setsuko Tsuboi and Takashi Jin
    • Journal Title

      ACS OMEGA

      Volume: 7 Issue: 2 Pages: 2015-2113

    • DOI

      10.1021/acsomega.1c05636

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shortwave-infrared (SWIR) emitting annexin V for high-contrast fluorescence molecular imaging of tumor apoptosis in living mice2022

    • Author(s)
      Swamy Mahadeva M. M.、Tsuboi Setsuko、Murai Yuta、Monde Kenji、Jin Takashi
    • Journal Title

      RSC Advances

      Volume: 12 Issue: 30 Pages: 19632-19639

    • DOI

      10.1039/d2ra03315a

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A near-infrared fluorescent long-chain fatty acid toward optical imaging of cardiac metabolism in living mice2022

    • Author(s)
      Swamy Mahadeva M. M.、Zubir Mohamad Zarif Mohd、Mutmainah、Tsuboi Setsuko、Murai Yuta、Monde Kenji、Hirano Ken-ichi、Jin Takashi
    • Journal Title

      The Analyst

      Volume: 147 Issue: 19 Pages: 4206-4212

    • DOI

      10.1039/d2an00999d

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shortwave-Infrared Fluorescent Molecular Imaging Probes Based on π-Conjugation Extended Indocyanine Green2021

    • Author(s)
      Mahadeva M. M. Swamy, Yuta Murai, Kenji Monde, Setsuko Tsuboi, and Takashi Jin
    • Journal Title

      Bioconjugate Chem.

      Volume: 32 Issue: 8 Pages: 1541-1547

    • DOI

      10.1021/acs.bioconjchem.1c00253

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent Gold Nanoclusters for In Vivo Shortwave-Infrared Imaging2021

    • Author(s)
      Setsuko Tsuboi and Takashi JIN
    • Journal Title

      ECS J. Solid State Sci. Technol.

      Volume: 10 Issue: 9 Pages: 096012-096012

    • DOI

      10.1149/2162-8777/ac258c

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shortwave-infrared (SWIR) fluorescence molecular imaging using indocyanine green-antibody conjugates for the optical diagnostics of cancerous tumours2020

    • Author(s)
      Setsuko Tsuboi and Takashi Jin
    • Journal Title

      RSC advances

      Volume: 10 Issue: 47 Pages: 28171-24179

    • DOI

      10.1039/d0ra04710d

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo2020

    • Author(s)
      Setsuko Tsuboi and Takashi Jin
    • Journal Title

      RSC advances

      Volume: 10 Issue: 63 Pages: 38244-38250

    • DOI

      10.1039/d0ra06495e

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging2020

    • Author(s)
      Dhermendra K. Tiwari, Manisha Tiwari and Takashi Jin
    • Journal Title

      Materials Advances

      Volume: 1 Issue: 5 Pages: 967-987

    • DOI

      10.1039/d0ma00273a

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Monte Carlo Modeling of Near-infrared Fluorescence Photon Migration in Breast Tissue for Tumor Prediction2020

    • Author(s)
      Tatsuto IIDA, Takashi JIN, Yasutomo NOMURA
    • Journal Title

      Advanced Biomedical Engineering

      Volume: 9 Issue: 0 Pages: 100-105

    • DOI

      10.14326/abe.9.100

    • NAID

      130007843383

    • ISSN
      2187-5219
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Monte Carlo Modeling of Shortwave-Infrared Fluorescence Photon Migration in Voxelized Media for the Detection of Breast Cancer2020

    • Author(s)
      Tatsuto Iida, Shunsuke Kiya, Kosuke Kubota, Takashi Jin, Akitoshi Seiyama and Yasutomo Nomura
    • Journal Title

      Diagnostics

      Volume: 10 Issue: 11 Pages: 961-961

    • DOI

      10.3390/diagnostics10110961

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Monte Carlo Evaluation of <i>In Vivo</i> Neuroimaging Using Quantum Dots with Fluorescence in the Second Window of Near Infrared Region2019

    • Author(s)
      Hiro Yamato, Tatsuto Iida, Takashi Jin, Yasutomo Nomura
    • Journal Title

      Advanced Biomedical Engineering

      Volume: 8 Issue: 0 Pages: 105-109

    • DOI

      10.14326/abe.8.105

    • NAID

      130007635369

    • ISSN
      2187-5219
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BRET-based dual-color (visible/near-infrared) molecular imaging using a quantum dot/EGFP-luciferase conjugate2019

    • Author(s)
      Tsuboi S, Jin T.
    • Journal Title

      RSC Adv

      Volume: 9 Issue: 60 Pages: 34964-34971

    • DOI

      10.1039/c9ra07011g

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optimal focus evaluated using Monte Carlo simulation in non-invasive neuroimaging in the second near-infrared window2019

    • Author(s)
      Hiro Yamato, Tatsuto Iida, Takashi Jin, Yasutomo Nomura
    • Journal Title

      Methods X

      Volume: 6 Pages: 2367-2373

    • DOI

      10.1016/j.mex.2019.09.010

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Near infrared imaging of intrinsic signals in cortical spreading depression observed through the intact scalp in hairless mice2019

    • Author(s)
      Hiro Yamato, Takashi Jin, Yasutomo Nomura
    • Journal Title

      Neurosci. Lett.,

      Volume: 701 Pages: 213-217

    • DOI

      10.1016/j.neulet.2019.02.034

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent recombinant protein conjugated near-infrared emitting quantum dots for in vitro and in vivo dual-color molecular imaging2019

    • Author(s)
      Tsuboi S, Jin T.
    • Journal Title

      ChemBioChem

      Volume: 20 Issue: 4 Pages: 565-575

    • DOI

      10.1002/cbic.201800506

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 10.1149/2.0111901jss2019

    • Author(s)
      Jin T.
    • Journal Title

      ECS J. Solid State Sci.Technol.

      Volume: 8 Issue: 1 Pages: R9-R13

    • DOI

      10.1149/2.0111901jss

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Book] SWIR Fluorescence and Monte Carlo Modeling of Tissues for Medical Applications (Chap 7)2022

    • Author(s)
      T. Iida, S. Kiya, K. Kubota, A. Seiyama, T. Jin, and Y. Nomura
    • Total Pages
      23
    • Publisher
      SPIE
    • ISBN
      9781510646230
    • Related Report
      2021 Annual Research Report
  • [Book] Bioluminescence, 4th Ed. 2022(Part I-5)2022

    • Author(s)
      Setsuko Tsuboi and Takashi Jin
    • Total Pages
      14
    • Publisher
      Springer
    • ISBN
      9781071624722
    • Related Report
      2021 Annual Research Report
  • [Book] Near Infrared Emitting Nanoparticles for Biomedical Applications2020

    • Author(s)
      Takashi Jin and Yasutomo Nomura
    • Publisher
      Springer
    • ISBN
      9783030320355
    • Related Report
      2020 Annual Research Report
  • [Book] Bioluminescent Imaging-Methods and Protocols2020

    • Author(s)
      Setsuko Tsuboi and Takashi Jin
    • Publisher
      Humana Press
    • ISBN
      9781493999392
    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] 化合物、その製造方法、複合体および短波赤外蛍光剤2020

    • Inventor(s)
      神 隆、マハデバ スワミー、門出健次、村井勇太
    • Industrial Property Rights Holder
      神 隆、マハデバ スワミー、門出健次、村井勇太
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-041717
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

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