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Molecular basis of bulk transport machinery playing key roles in lipid secretion in plant cells

Research Project

Project/Area Number 19H05638
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Review Section Broad Section F
Research InstitutionKyoto University

Principal Investigator

Yazaki Kazufumi  京都大学, 生存圏研究所, 教授 (00191099)

Co-Investigator(Kenkyū-buntansha) 粟野 達也  京都大学, 農学研究科, 助教 (40324660)
Project Period (FY) 2019-06-26 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥165,620,000 (Direct Cost: ¥127,400,000、Indirect Cost: ¥38,220,000)
Fiscal Year 2023: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2022: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Fiscal Year 2021: ¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2020: ¥21,450,000 (Direct Cost: ¥16,500,000、Indirect Cost: ¥4,950,000)
Fiscal Year 2019: ¥76,700,000 (Direct Cost: ¥59,000,000、Indirect Cost: ¥17,700,000)
Keywords植物細胞 / 脂質分泌 / バルク輸送 / ABCトランスポータ / 膜ダイナミクス / シコニン / 二次代謝 / トリアシルグリセロール
Outline of Research at the Start

植物は自らの生命維持に必須なワックス成分だけでなく、抗がん剤のビンクリスチンやタキソール、また香り成分のモノテルペンや防御物質のクマリンなど、数多くの脂溶性低分子を細胞外に分泌する。しかし、植物は水に溶けない物質をどのようにして細胞外に大量に出せるのか、その分子機構は大きな謎である。
本研究は、ムラサキという好適モデルを用いて、この大きな謎を解明することを目指す。

Outline of Final Research Achievements

Plants secrete a variety of secondary metabolites with different biological activities from specific cell types or specialized tissues into the extracellular space, where they accumulate in the apoplast. The aim of this study was to elucidate the molecular mechanisms of the transport events involved in extracellular secretion, especially for the enigmatic lipid-soluble substances. In this study, we used the lipid-soluble red pigment shikonin and the cultured cells and hairy roots of Lithospermum erythrorhizon (gromwell, Boraginaceae), which secrete 10% of it per cell, as models. We identified several proteins relevant for membrane dynamics, such as ENTH/VHS and epsin-2, and G-type ABC transporters (ABCG) as membrane transporters that would be important for the secretion of lipid-soluble metabolites. We then elucidated their functions at the molecular level in heterologous plant species.

Academic Significance and Societal Importance of the Research Achievements

今から4億5千万年前、植物が地上に進出し種の繁栄を達成できたのは、大気中で自らの体を乾燥から守る、という新機能であった。それはワックスやスベリンといった脂溶性ポリマーで細胞表面を覆うことで、水の蒸散を防ぐ機能の獲得である。また植物は香り成分や抗癌成分などを生産するが、それら低分子の機能性成分の多くはやはり脂溶性で、細胞外で蓄積するものが多い。しかしその分泌メカニズムは謎に包まれていた。本研究では、こうした脂溶性物質の細胞外分泌に焦点を当て、分泌過程にかかわる膜交通系タンパク質や輸送体、またマトリックス脂質分子を同定し、その実態に迫った。

Assessment Rating
Ex-post Assessment Comments (Rating)

B: In light of the aim of introducing the research area into the research categories, research outcomes have been produced to a certain extent, but not sufficient.

Assessment Rating
Interim Assessment Comments (Rating)

A: In light of the aim of introducing the research area into the research categories, the expected progress has been made in research.

Report

(11 results)
  • 2024 Ex-post Assessment (Comments) ( PDF )
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Abstract (Interim Assessment) ( PDF )   Annual Research Report   Interim Assessment (Comments) ( PDF )
  • 2020 Annual Research Report
  • 2019 Abstract ( PDF )   Comments on the Screening Results ( PDF )   Annual Research Report
  • Research Products

    (63 results)

All 2024 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (1 results) Journal Article (24 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 24 results,  Open Access: 14 results) Presentation (35 results) (of which Int'l Joint Research: 1 results,  Invited: 10 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] University of Lorraine/INRAE/Laboratory of Agronomy and Environment(フランス)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Peroxisomal 4-coumaroyl-CoA ligases participate in shikonin production in <i>Lithospermum erythrorhizon</i>2024

    • Author(s)
      Nakanishi Kohei、Li Hao、Ichino Takuji、Tatsumi Kanade、Osakabe Keishi、Watanabe Bunta、Shimomura Koichiro、Yazaki Kazufumi
    • Journal Title

      Plant Physiology

      Volume: - Issue: 4 Pages: 2843-2859

    • DOI

      10.1093/plphys/kiae157

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] omparative analysis of shikonin and alkannin acyltransferases reveals their functional conservation in Boraginaceae2024

    • Author(s)
      Oshikiri H., Li H., Manabe M., Yamamoto H., Yazaki K., Takanashi K.
    • Journal Title

      Plant and Cell Physiology

      Volume: 65 Issue: 3 Pages: 362-371

    • DOI

      10.1093/pcp/pcad158

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Excretion of triacylglycerol as a matrix lipid facilitating apoplastic accumulation of a lipophilic metabolite shikonin2023

    • Author(s)
      Kanade Tatsumi; Takuji Ichino; Natsumi Isaka; Akifumi Sugiyama; Eiko Moriyoshi; Yozo Okazaki; Yasuhiro Higashi; Masataka Kajikawa; Yoshinori Tsuji; Hideya Fukuzawa et al.
    • Journal Title

      Journal of Experimental Botany

      Volume: 74 Issue: 1 Pages: 104-117

    • DOI

      10.1093/jxb/erac405

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Apoplast-localized β-glucosidase elevates isoflavone accumulation in the soybean rhizosphere2023

    • Author(s)
      Matsuda, H., Yamazaki, Y., Moriyoshi, E., Nakayasu, M., Yamazaki, S., Aoki, Y., Takase, H., Okazaki, S., Nagano, A.J., Kaga, A., Yazaki, K., Sugiyama, A.
    • Journal Title

      Plant and Cell Physiology

      Volume: in press

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gromwell, a purple link between traditional Japanese culture and plant science2023

    • Author(s)
      Emi Ito, Ryosuke Munakata, Kazufumi Yazaki
    • Journal Title

      Plant and Cell Physiology

      Volume: in press

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inventory of ATP-binding cassette proteins in<i>Lithospermum erythrorhizon</i>as a model plant producing divergent secondary metabolites2022

    • Author(s)
      Li Hao、Matsuda Hinako、Tsuboyama Ai、Munakata Ryosuke、Sugiyama Akifumi、Yazaki Kazufumi
    • Journal Title

      DNA Research

      Volume: 29 Issue: 3 Pages: 1-12

    • DOI

      10.1093/dnares/dsac016

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Plant specialized metabolites in the rhizosphere of tomatoes: secretion and effects on microorganisms2022

    • Author(s)
      Nakayasu Masaru、Takamatsu Kyoko、Yazaki Kazufumi、Sugiyama Akifumi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 87 Issue: 1 Pages: 13-20

    • DOI

      10.1093/bbb/zbac181

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 合成生物学を利用したメロテルペン類とアルカロイドの微生物生産2022

    • Author(s)
      棟方涼介、矢﨑一史
    • Journal Title

      植物の生長調節

      Volume: 57 Pages: 93-99

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved Chemical Fixation of Lipid-Secreting Plant Cells for Transmission Electron Microscopy2022

    • Author(s)
      Kiyoto Shingo、Ichino Takuji、Awano Tatsuya、Yazaki Kazufumi
    • Journal Title

      Microscopy

      Volume: Online ahead of print Issue: 4 Pages: 206-213

    • DOI

      10.1093/jmicro/dfac018

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Modes of secretion of plant lipophilic metabolites via ABCG transporter-dependent transport and vesicle-mediated trafficking2022

    • Author(s)
      Ichino Takuji、Yazaki Kazufumi
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 66 Pages: 102184-102184

    • DOI

      10.1016/j.pbi.2022.102184

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Active Transport of Lignin Precursors into Membrane Vesicles from Lignifying Tissues of Bamboo2021

    • Author(s)
      Shimada Natsumi、Munekata Noriaki、Tsuyama Taku、Matsushita Yasuyuki、Fukushima Kazuhiko、Kijidani Yoshio、Takabe Keiji、Yazaki Kazufumi、Kamei Ichiro
    • Journal Title

      Plants

      Volume: 10 Issue: 11 Pages: 2237-2237

    • DOI

      10.3390/plants10112237

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of raspberry ketone by redirecting the metabolic flux to the phenylpropanoid pathway in tobacco plants2021

    • Author(s)
      Koeduka Takao、Takarada Sachiho、Fujii Koya、Sugiyama Akifumi、Yazaki Kazufumi、Nishihara Masahiro、Matsui Kenji
    • Journal Title

      Metabolic Engineering Communications

      Volume: 13 Pages: e00180-e00180

    • DOI

      10.1016/j.mec.2021.e00180

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ムラサキ科植物におけるシコニン生合成研究の新潮流2021

    • Author(s)
      矢﨑 一史
    • Journal Title

      Farumashia

      Volume: 57 Issue: 8 Pages: 705-709

    • DOI

      10.14894/faruawpsj.57.8_705

    • NAID

      130008070453

    • ISSN
      0014-8601, 2189-7026
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants2021

    • Author(s)
      Munakata, R., Olry, A., Takemura, T., Tatsumi, K., Ichino, T., Villard, C., Kageyama, J., Kurata, T., Nakayasu, M., Jacob, F., Koeduka, T., Yamamoto, H., Moriyoshi, E., Matsukawa, T., Grosjean, J., Krieger, C., Sugiyama, A., Mizutani, M., Bourgaud, F., Hehn, A., Yazaki, K.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 118 Issue: 17

    • DOI

      10.1073/pnas.2022294118

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Highly efficient method of <i>Lithospermum erythrorhizon</i> transformation using domestic <i>Rhizobium rhizogenes</i> strain A132020

    • Author(s)
      Kanade Tatsumi, Takuji Ichino, Noboru Onishi, Koichiro Shimomura, Kazufumi Yazaki
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 1 Pages: 39-46

    • DOI

      10.5511/plantbiotechnology.19.1212a

    • NAID

      130007822563

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2020-03-25
    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alcohol Dehydrogenase Activity Converts 3″-Hydroxy-geranylhydroquinone to an Aldehyde Intermediate for Shikonin and Benzoquinone Derivatives in Lithospermum erythrorhizon2020

    • Author(s)
      Yamamoto Hirobumi、Tsukahara Mika、Yamano Yumiko、Wada Akimori、Yazaki Kazufumi
    • Journal Title

      Plant and Cell Physiology

      Volume: 61 Issue: 10 Pages: 1798-1806

    • DOI

      10.1093/pcp/pcaa108

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Apple latent spherical virus (ALSV)-induced gene silencing in a medicinal plant Lithospermum erythrorhizon2020

    • Author(s)
      Izuishi Yuki, Isaka Natsumi, Li Hao, Nakanishi Kohei, Kageyama Joji, Ishikawa Kazuya, Shimada Tomoo, Masuta Chikara, Yoshikawa Nobuyuki, Kusano Hiroaki, Yazaki Kazufumi
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 13555-13555

    • DOI

      10.1038/s41598-020-70469-1

    • NAID

      120007175169

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two BAHD Acyltransferases Catalyze the Last Step in the Shikonin/Alkannin Biosynthetic Pathway2020

    • Author(s)
      Haruka Oshikiri, Bunta Watanabe, Hirobumi Yamamoto, Kazufumi Yazaki, Kojiro Takanashi
    • Journal Title

      Plant Physiology

      Volume: 184 Issue: 2 Pages: 753-761

    • DOI

      10.1104/pp.20.00207

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Cytosol-Localized Geranyl Diphosphate Synthase from Lithospermum erythrorhizon and Its Molecular Evolution2020

    • Author(s)
      Ueoka Hayato、Sasaki Kanako、Miyawaki Tatsuya、Ichino Takuji、Tatsumi Kanade、Suzuki Shiro、Yamamoto Hirobumi、Sakurai Nozomu、Suzuki Hideyuki、Shibata Daisuke、Yazaki Kazufumi
    • Journal Title

      Plant Physiology

      Volume: 182 Issue: 4 Pages: 1933-1945

    • DOI

      10.1104/pp.19.00999

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamism of vacuoles toward survival strategy in plants2019

    • Author(s)
      Shitan Nobukazu、Yazaki Kazufumi
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Biomembranes

      Volume: 15 Issue: 12 Pages: 183127-183127

    • DOI

      10.1016/j.bbamem.2019.183127

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolutionary Developments in Plant Specialized Metabolism, Exemplified by Two Transferase Families2019

    • Author(s)
      Kusano Hiroaki、Li Hao、Minami Hiroshi、Kato Yoshihiro、Tabata Homare、Yazaki Kazufumi
    • Journal Title

      Frontiers in Plant Science

      Volume: 10 Pages: 794-794

    • DOI

      10.3389/fpls.2019.00794

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isolation of Artemisia capillaris membrane-bound di-prenyltransferase for phenylpropanoids and redesign of artepillin C in yeast2019

    • Author(s)
      Munakata Ryosuke、Takemura Tomoya、Tatsumi Kanade、Moriyoshi Eiko、Yanagihara Koki、Sugiyama Akifumi、Suzuki Hideyuki、Seki Hikaru、Muranaka Toshiya、Kawano Noriaki、Yoshimatsu Kayo、Kawahara Nobuo、Yamaura Takao、Grosjean Jeremy、Bourgaud Frederic、Hehn Alain、Yazaki Kazufumi
    • Journal Title

      Communications Biology

      Volume: 2 Issue: 1 Pages: 384-384

    • DOI

      10.1038/s42003-019-0630-0

    • NAID

      120006733113

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Convergent evolution of the UbiA prenyltransferase family underlies the independent acquisition of furanocoumarins in plants2019

    • Author(s)
      Munakata Ryosuke、Kitajima Sakihito、Nuttens Andreina、Tatsumi Kanade、Takemura Tomoya、Ichino Takuji、Galati Gianni、Vautrin Sonia、Berges Helene、Grosjean Jeremy、Bourgaud Frederic、Sugiyama Akifumi、Hehn Alain、Yazaki Kazufumi
    • Journal Title

      New Phytologist

      Volume: 225 Issue: 5 Pages: 2166-2182

    • DOI

      10.1111/nph.16277

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Proton Gradient-Dependent Transport of p-Glucocoumaryl Alcohol in Differentiating Xylem of Woody Plants2019

    • Author(s)
      Tsuyama Taku、Matsushita Yasuyuki、Fukushima Kazuhiko、Takabe Keiji、Yazaki Kazufumi、Kamei Ichiro
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 8900-8900

    • DOI

      10.1038/s41598-019-45394-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ムラサキのシコニン生産と同調して発現するポリフェノールオキシダーゼの機能解析2024

    • Author(s)
      中西浩平、高野祐希、山本恭子、佐々木佳菜子、小原一朗、市野琢爾、巽 奏、李 豪、棟方涼介、山本浩文、刑部敬史、下村講一郎、杉山暁史、高梨功次郎、矢崎一史
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ムラサキ科植物のシコニン・アルカニン誘導体生合成に関与するアシル基転移酵素の比較解析2024

    • Author(s)
      押切春佳、李 豪、眞辺美咲、山本浩文、矢崎一史、高梨功次郎
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ムラサキ培養細胞から分泌されるシコニン含有顆粒はアラビノガラクタンタンパク質に覆われペクチンマトリックスに包埋されている2024

    • Author(s)
      松岡 健、市野琢爾、中西浩平、矢崎一史
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ムラサキ毛状根における脂質分泌膜系の透過電子顕微鏡観察2024

    • Author(s)
      清都晋吾、粟野達也、市野 琢爾、矢﨑一史
    • Organizer
      第74 回日本木材学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] イチゴのテルペン系香気成分の分泌を担う候補遺伝子の解析2024

    • Author(s)
      後藤桃佳、段 奈々子、上岡颯人、李 豪、橘 頼之、市野琢爾、杉山暁史、棟方涼介、矢﨑一史
    • Organizer
      日本農芸化学会 2024年度大会(東京)
    • Related Report
      2023 Annual Research Report
  • [Presentation] 二次代謝のモデル植物ムラサキのABCタンパク質の解析2023

    • Author(s)
      近藤菜友、坪山 愛、市野琢爾、李 豪、巽 奏、松田陽菜子、刑部敬史、下村講一郎、棟方 涼介、杉山 暁史、矢崎 一史
    • Organizer
      第40回植物バイオテクノロジー学会(千葉)大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ムラサキのシコニン生合成中間体を基質とするshort-chain dehydrogenase/ reductaseの機能解析2023

    • Author(s)
      松田悠希、吉岡奈津子、渡辺文太、市野 琢爾、山本 浩文、矢崎一史、高梨功次郎
    • Organizer
      第40回植物バイオテクノロジー学会(千葉)大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] シコニン・アルカニン誘導体生合成に関わるアシル基転移酵素の機能比較2023

    • Author(s)
      押切春佳、李 豪、山本浩文、矢崎一史、高梨功次郎
    • Organizer
      第40回植物バイオテクノロジー学会(千葉)大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 日本文化を支えた『紫』の染料植物2023

    • Author(s)
      矢崎一史
    • Organizer
      グリーンエネルギーファーム産学共創パートナーシップ
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] ムラサキにおける細胞外ナフトキノンと分泌型ペルオキシダーゼの生理的役割2023

    • Author(s)
      市野 琢爾, 陽川 憲, 巽 奏, 晝間 敬, 中安 大, 高松 恭子, 森吉 英子, 棟方 有桂, 杉山 暁史, 渡邊 崇人, 下村 講一郎, 渡辺 隆司, 矢﨑 一史
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] wo peroxisomal 4-coumaroyl-CoA ligases are involved in shikonin biosynthesis of Lithospermum erythrorhizon2023

    • Author(s)
      中西浩平、李豪、市野琢爾、巽奏、刑部敬史、渡辺文太、下村講一郎、矢崎一史
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 薬効成分の生合成と蓄積の分子機構 ―シコニンを緒として―2022

    • Author(s)
      矢崎一史
    • Organizer
      日本生薬学会第68回年会(松山)
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] ムラサキ培養細胞における脂溶性色素シコニンの生産とトリアシルグリセロールの分泌2022

    • Author(s)
      矢﨑一史、巽 奏、市野琢爾、清都晋吾、粟野達也、太田啓之、斉藤和季
    • Organizer
      第34回植物脂質化学シンポジウム(京都)
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 植物フェノール類の生理活性向上の鍵を握る二次代謝系プレニル化酵素2022

    • Author(s)
      矢崎一史
    • Organizer
      第32回イソプレノイド研究会(新潟)
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 植物細胞における脂溶性物質の蓄積と分泌2022

    • Author(s)
      矢崎一史
    • Organizer
      第3回天然ゴム研究会(仙台)
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] ムラサキのシコニン生合成に関わる2つの4-coumaroyl-CoA ligaseの機能特性2022

    • Author(s)
      中西浩平、李豪、市野琢爾、巽奏、刑部敬史、渡辺文太、下村講一郎、矢崎一史
    • Organizer
      第39回日本植物バイオテクノロジー学会(堺)大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 薬用植物ムラサキにおける2つのハーフサイズABCG輸送体の解析2022

    • Author(s)
      市野 琢爾, 巽 奏, 棟方 有桂, 坪山 愛, 森吉 英子, 中安 大, 高梨 功次郎, 矢﨑 一史
    • Organizer
      第39回日本植物バイオテクノロジー学会(堺)大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ムラサキ細胞のシコニン系脂溶性化合物の分泌における膜融合関連 タンパク質EXO70の解析2022

    • Author(s)
      正木泰斗、市野琢爾、豊田健人、下村講一郎、矢崎一史
    • Organizer
      第34回植物脂質化学シンポジウム(京都)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 二次代謝のモデル植物ムラサキのABCタンパク質の解析2022

    • Author(s)
      坪山 愛、李 豪、市野 琢爾、松田 陽菜子、棟方 涼介、杉山 暁史、矢﨑 一史
    • Organizer
      日本農芸化学会2022年度(京都大会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] シコニン関連遺伝子を標的としたムラサキのゲノム編集毛状根の評価2022

    • Author(s)
      李 豪、市野 琢爾、中西 浩平、草野 博彰、刑部 敬史、下村 講一郎、矢崎 一史
    • Organizer
      日本農芸化学会2022年度(京都大会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] 揮発性テルペノイドの分泌に関わる候補因子の探索と基盤構築2022

    • Author(s)
      段 奈々子、上岡 颯人、李 豪、橘 頼之、杉山 暁史、棟方 涼介、矢﨑 一史
    • Organizer
      日本農芸化学会2022年度(京都大会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] シコニン/アルカニン生合成経路で働くデオキシシコニン水酸化酵素の機能解析2022

    • Author(s)
      大塚 峻、市野 琢爾、吉村 和貴、李 豪、山本 浩文、矢崎 一史
    • Organizer
      日本農芸化学会2022年度(京都大会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] ムラサキ代謝における4-coumaroyl-CoA ligaseの機能分担とシコニン生合成への関与2022

    • Author(s)
      中西 浩平、李 豪、市野 琢爾、巽 奏、刑部 敬史、渡辺 文太、下村 講一郎、矢崎 一史
    • Organizer
      日本農芸化学会2022年度(京都大会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] 代謝産物の分泌能向上にむけたムラサキ由来脂質分泌遺伝子候補の異種発現2021

    • Author(s)
      市野 琢爾、棟方(齊田) 有桂、大島 良美、金沢 香織、森吉 英子、巽 奏、光田 展隆、矢崎 一史
    • Organizer
      第38回日本植物バイオテクノロジー学会・つくば大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 薬用植物ムラサキにおける4-coumaroyl-CoA ligaseの果たす役割2021

    • Author(s)
      中西 浩平、李 豪、市野 琢爾、巽 奏、刑部 敬史、渡辺 文太、下村 講一郎、矢崎 一史
    • Organizer
      第38回日本植物バイオテクノロジー学会・つくば大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ムラサキにおけるシコニン分泌機構の解明と植物の輸送能力の改変にむけた利用2021

    • Author(s)
      市野 琢爾、棟方 有桂、大島 良美、金沢 香織、巽 奏、矢崎 一史
    • Organizer
      第33回植物脂質シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Characterization of 4-coumaroyl-CoA ligase (4CL) involved in shikoninbiosynthesis in Lithospermum erythrorhizon2021

    • Author(s)
      Kohei Nakanishi, Hao Li, Keishi Osakabe, Bunta Watanabe, Kazufumi Yazaki
    • Organizer
      11th Synposium of the International Society of Root Research
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マラカイトグリーン、イミダゾール緩衝オスミウム、p-フェニレンジアミンの組み合わせによる化学固定法の改良2021

    • Author(s)
      清都晋吾、粟野達也、矢崎一史
    • Organizer
      第71 回日本木材学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 脂溶性二次代謝産物シコニンの細胞外蓄積に関与する分泌脂質分子の解析2021

    • Author(s)
      巽 奏、市野琢爾、東 泰弘、井坂夏海、上撫健太、梶川昌孝、福澤秀哉、豊岡公徳、佐藤繭子、市 育代、斉藤和季、矢崎一史
    • Organizer
      第61 回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 植物における代謝産物の蓄積機構の制御技術の開発2020

    • Author(s)
      矢崎一史
    • Organizer
      BioJapan 2020
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 植物フェノール類プレニル化酵素の位置特異性を担う触媒機構の解明に向けて2020

    • Author(s)
      棟方涼介、高橋宏暢、肥塚崇男、Alain HEHN、矢﨑一史
    • Organizer
      第30回イソプレノイド研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 生合成工学と輸送工学を統合したプレニル化ポリフェノールの生合成リデザイン2019

    • Author(s)
      矢崎一史
    • Organizer
      新学術領域研究 生物合成系の再設計による複雑骨格機能分子の革新的創成科学、第6回公開シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 植物の脂溶性テルペン化合物の細胞外分泌2019

    • Author(s)
      矢崎一史
    • Organizer
      第2回天然ゴム研究会/理研シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 物質集積メカニズムの制御による高次物質生産技術 -植物における代謝産物の蓄積機構の制御技術の開発-2019

    • Author(s)
      矢崎一史
    • Organizer
      BioJapan 2019 NEDOセミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 植物の鉄要求性を支える膜輸送体とその生理機能2019

    • Author(s)
      矢崎一史
    • Organizer
      第43回日本鉄バイオサイエンス学会学術集会 シンポジウム2
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Book] 植物バイオテクノロジーでめざすSDGs(担当:第10章「薬の歴史 ―植物化学とのつながりを紐解く)2023

    • Author(s)
      矢崎一史 (編:小泉 望、加藤 晃)
    • Total Pages
      184
    • Publisher
      化学同人
    • ISBN
      9784759820867
    • Related Report
      2022 Annual Research Report
  • [Remarks] 森林圏遺伝子統御分野 矢崎研究室

    • URL

      https://www.rish.kyoto-u.ac.jp/lpge/

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
  • [Remarks] 森林圏遺伝子統御分野 矢崎研究室

    • URL

      http://www.rish.kyoto-u.ac.jp/lpge/

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2019-06-27   Modified: 2025-02-04  

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