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to understand immune dysregulation associated with dysbiosis in necrotizing enterocolitis

Research Project

Project/Area Number 19J10790
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Review Section Basic Section 55010:General surgery and pediatric surgery-related
Research InstitutionOsaka University

Principal Investigator

李 波  大阪大学, 医学系研究科, 特別研究員(PD)

Project Period (FY) 2019-04-25 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2020: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2019: ¥900,000 (Direct Cost: ¥900,000)
KeywordsE-NTPD8 / P2X4 / ATP / DSS-induced colitis / 壊死性腸炎 / 組換えヒト可溶性トロンボモジュリン(rTM) / ラットモデル
Outline of Research at the Start

1). Generate gnotobiotic mice colonized with human breast milk fed preterm infant microbiota (HMb) or formula fed preterm infant microbiota (FMb).
2). Explore how HMb mice and FMb mice differ in susceptibility to NEC-like injury.
3). Reveal causal bacterial family associated with survival from NEC.

Outline of Annual Research Achievements

新生児ラットモデルを用いて、壊死性腸炎に対して組換えヒト可溶性トロンボモジュリンは効果があることがわかりました。研究結果はSurgery Todayに掲載されました。その後腸管免疫に関する研究を行いました。
要約は以下の通りです。
We show that E-NTPD8 is highly expressed in large intestinal epithelial cells and hydrolyzes microbiota-derived luminal adenosine triphosphate ATP. Compared to wild-type mice, Entpd8-/- mice develop more severe DSS-induced colitis. In this context, either depletion of neutrophils and monocytes by injecting with anti-Gr-1 antibody or introduction of P2rx4 deficiency into hematopoietic cells relieve colitis in Entpd8-/- mice. Increased level of luminal ATP in the colon of Entpd8-/- mice promotes glycolysis in neutrophils and monocytes through P2X4 receptor-dependent Ca2+ influx, which links to prolonged survival and elevated ROS production in these cells.
今論文は投稿中です。

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Report

(2 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (3 results)

All 2019

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Recombinant human soluble thrombomodulin reduces the severity and incidence of necrotizing enterocolitis in a newborn rat model2019

    • Author(s)
      Bo Li, Ryuta Saka, Yuichi Takama, Takehisa Ueno, Yuko Tazuke, Hiroomi Okuyama
    • Journal Title

      Surgery Today

      Volume: 49 Issue: 11 Pages: 971-976

    • DOI

      10.1007/s00595-019-01832-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Recombinant human soluble thrombomodulin reduces the severity and incidence of necrotizing enterocolitis in a newborn rat model2019

    • Author(s)
      Bo Li and Hiroomi Okuyama
    • Organizer
      2019NEC Society
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Deubiquitinase Otud3-mediated suppression of intestinal inflammation2019

    • Author(s)
      Bo Li and Kiyoshi Takeda
    • Organizer
      第48回日本免疫学会学術集会
    • Related Report
      2019 Annual Research Report

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Published: 2019-05-29   Modified: 2024-03-26  

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