| Project/Area Number |
19J11493
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 39050:Insect science-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
JANG Seonghan 北海道大学, 農学院, 特別研究員(DC2)
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| Project Period (FY) |
2019-04-25 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2020: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2019: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Symbiosis / Burkholderia / Riptortus pedestris / Duox / Dual oxidase / Mutualism / Microbiology / symbiosis / symbiont / Insect |
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| Outline of Research at the Start |
Gut microbiota is important for many organisms including human. Especially "symbiont bacteria" can give many beneficial effects as like enhancement of immunity. By using the insect-bacteria symbiosis system, we can understand deeply about host-symbiont interaction
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| Outline of Annual Research Achievements |
In this fiscal year, I elucidated novel function of NADPH oxidase enzyme, Dual oxidase (Duox), in a pest insect Riptortus pedestris. Duox is originally well-known enzyme related to gut mucosal immunity of insects. Upon intestinal infection of pathogens, Duox generates bactericidal reactive oxygen species (ROS) and kill invaded pathogens. However, in R. pdestris Duox was not related to gut immunity, but it stabilized insect's respiratory network by cross-linking tyrosine bonds of proteins resulting in forming dityrosine network (DTN). Genetic blocking of duox gene indeed destabilized a DTN and it caused the collapse of insect respiratory network. Thus, aerobic symbiont Burkholderia were died from the midgut since they can't utilize enough oxygen. This study was published in PNAS journal.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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