| Project/Area Number |
19J22546
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
|
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| Research Institution | The University of Tokyo |
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Principal Investigator |
寿 景文 東京大学, 工学系研究科, 特別研究員(DC1)
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| Project Period (FY) |
2019-04-25 – 2022-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2021: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2020: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2019: ¥900,000 (Direct Cost: ¥900,000)
|
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| Keywords | super-multiplex / super-resolution / stimulated Raman / fluorescence / high-speed / Super-multiplex / Stimulated Raman / Fluorescence / High-speed |
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| Outline of Research at the Start |
Biological systems are subject to the complicated and dynamic interplay. The organelle contacts play a vital role in diverse cellular function. In this research, high-speed super-multiplexed imaging will be developed and exploited for the co-localization imaging and interactome anlysis.
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| Outline of Annual Research Achievements |
This year, we have worked on the theme of ‘ super-multiplex biological imaging’ mainly towards super-multiplex super-resolution imaging. Via applying the technology of photoswitchable stimulated Raman scattering microscopy developed before which is also a part of this project, we successfully demonstrate super-resolution stimulated Raman scattering imaging with high chemical specificity. Meanwhile, the newly developed photoswitchable Raman probe enable us to target certain kind of intracellular organelle for meaningful biological imaging. The evaluated spatial resolution has broken the diffraction limitation in the circumstance of vibrational imaging. Both the narrow linewidth and super-resolution property shows the developed technique as an important step towards final super-multiplex super-resolution imaging.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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