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遺伝的同化理論を考慮した洞窟性魚類の適応進化機構の解明

Research Project

Project/Area Number 19J22686
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionUniversity of the Ryukyus

Principal Investigator

小林 大純  琉球大学, 理工学研究科海洋環境学専攻, 特別研究員(DC1)

Project Period (FY) 2019-04-25 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2021: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2019: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords適応 / ノコギリハゼ科 / カワアナゴ科 / 系統 / 洞窟 / ハゼ目 / 両側回遊 / 洞窟性生物 / ゲノミクス / 退化
Outline of Research at the Start

近年遺伝的同化が進化に果たした役割が再認識されつつあるが,これを野外で分子基盤を解明した研究はない.申請者の研究で,カワアナゴ科魚類には地上と洞窟の両環境に応じて表現型を変化させる種が存在し洞窟性種の進化に表現系可塑性が関与した可能性が示唆された.本研究では,本科魚類における遺伝的同化仮説を検証するため,まず本科各種の全ゲノム配列を決定し種系統樹を解明する.次に本科各種の表現型可塑性の程度を共通環境実験によって評価し可塑性の進化過程について考察する.さらに発現変動遺伝子解析を行い可塑性の進化の分子基盤を解明する.また適応形質の祖先形質推定を行い,洞窟性種の進化史における前適応の要否を検討する.

Outline of Annual Research Achievements

本年度は当時の社会状況の影響を受けて変更された研究計画に基づき,本研究対象のカワアナゴ科,ノコギリハゼ科の外群となる近縁種(7属12種)を琉球列島(沖縄島・石垣島・西表島)および日本本土(九州・本州)で収集し,うち特に近縁なカワアナゴ科とノコギリハゼ科7種(カワアナゴ属4種,ジャノメハゼ属3種)の全ゲノムリシーケンス配列を次世代シーケンサーによって新規で取得した.得られたリシーケンスデータおよび昨年までの解析で得られた4種(カワアナゴ属3種およびジャノメハゼ属1種)のリシーケンスデータを,昨年までに作成されたテンジクカワアナゴのリファレンスゲノムおよび先行研究で公開されている中国産ジャノメハゼのリファレンスゲノムにそれぞれマッピングし,各種のミトコンドリアゲノムと全ゲノムSNPsのデータをそれぞれ取得した.これらのデータを統合し,ゲノム規模での系統樹を各属で構築することやPSMCによる種ごとの集団動態推定を行うことができ,現在より詳細な解析を進めている.また,これらの解析の過程で,ジャノメハゼ属と関連する近縁な2属(ホシマダラハゼ属,Paloa属)にそれぞれ未記載種と日本未記録種が発見され,カワアナゴ,ジャノメハゼの各属内にもそれぞれ1種の隠蔽種が発見された.本年度はこれらについても分類学的再検討を完了し,上記の系統解析で洞窟性種と比較すべき集団の再定義と形態データの追加取得を行うことができた.

Research Progress Status

令和3年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和3年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (28 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (6 results) Journal Article (13 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 13 results,  Open Access: 8 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Int'l Joint Research] Sam Ratulangi University/Halu Oleo University/Hasanuddin University(インドネシア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 国立ハノイ教育大学(ベトナム)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Western Philippines University(フィリピン)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] サムラトゥランギ大学(インドネシア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Sam Ratulangi University/Halu Oleo University/Hasanuddin University(インドネシア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Hanoi University of Education(ベトナム)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] New distributional records of two amphidromous sleepers, <i>Eleotris fusca</i> (Bloch and Schneider, 1801) and <i>Eleotris acanthopoma</i> Bleeker, 1853 from Ibaraki Prefecture, east coast of Japan2022

    • Author(s)
      小林 大純、山川 宇宙、内田 大貴、碧木 健人、外山 太一郎
    • Journal Title

      Ichthy, Natural History of Fishes of Japan

      Volume: 16 Issue: 0 Pages: 5-10

    • DOI

      10.34583/ichthy.16.0_5

    • NAID

      130008139563

    • ISSN
      2435-7715
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] First specimen-based records of two deepwater fishes, <i>Vinciguerria numbaria</i> (Jordan and Williams,1895) and <i>Hygophum proximum</i> Becker,1965 from Tonaki-jima Island, Okinawa Group, Ryukyu Islands, Japan2021

    • Author(s)
      小林 大純、内田 晃士
    • Journal Title

      Journal of Japan Driftological Society

      Volume: 19 Issue: 0 Pages: 17-19

    • DOI

      10.57279/driftological.19.0_17

    • NAID

      40022800803

    • ISSN
      1349-1555, 2436-8970
    • Year and Date
      2021-12-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two new species of Rhinogobius (Gobiiformes: Oxudercidae) from Palawan, Philippines, with their phylogenetic placement2021

    • Author(s)
      MAEDA KEN、SHINZATO CHUYA、KOYANAGI RYO、KUNISHIMA TAIGA、KOBAYASHI HIROZUMI、SATOH NORIYUKI、PALLA HERMINIE P.
    • Journal Title

      Zootaxa

      Volume: 5068 Issue: 1 Pages: 81-98

    • DOI

      10.11646/zootaxa.5068.1.3

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Do colour-morphs of an amphidromous goby represent different species? Taxonomy of Lentipes (Gobiiformes) from Japan and Palawan, Philippines, with phylogenomic approaches2021

    • Author(s)
      Ken Maeda, Hirozumi Kobayashi, Herminie P. Palla, Chuya Shinzato, Ryo Koyanagi, Javier Montenegro, Atsushi J. Nagano, Toshifumi Saeki, Taiga Kunishima, Takahiko Mukai, Katsunori Tachihara, Vincent Laudet, Noriyuki Satoh, Kazunori Yamahira
    • Journal Title

      SYSTEMATICS AND BIODIVERSITY

      Volume: 19 Issue: 8 Pages: 1080-1112

    • DOI

      10.1080/14772000.2021.1971792

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Species divergence and repeated ancient hybridization in a Sulawesian lake system2021

    • Author(s)
      Ixchel F. Mandagi, Ryo Kakioka, Javier Montenegro, Hirozumi Kobayashi, Kawilarang W. A. Masengi, Nobuyuki Inomata, Atsushi J. Nagano, Atsushi Toyoda, Satoshi Ansai, Masatoshi Matsunami, Ryosuke Kimura, Jun Kitano, Junko Kusumi, Kazunori Yamahira
    • Journal Title

      JOURNAL OF EVOLUTIONARY BIOLOGY

      Volume: 34 Issue: 11 Pages: 1767-1780

    • DOI

      10.1111/jeb.13932

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mesozoic origin and 'out-of-India' radiation of ricefishes (Adrianichthyidae)2021

    • Author(s)
      Kazunori Yamahira, Satoshi Ansai, Ryo Kakioka, et al.
    • Journal Title

      BIOLOGY LETTERS

      Volume: 17 Issue: 8 Pages: 20210212-20210212

    • DOI

      10.1098/rsbl.2021.0212

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Resource partitioning is not coupled with assortative mating in sympatrically divergent ricefish in a Wallacean ancient lake2021

    • Author(s)
      Ryo Kakioka, Nobu Sutra, Hirozumi Kobayashi, Satoshi Ansai, Kawilarang W. A. Masengi, Atsushi J. Nagano, Noboru Okuda, Rieko Tanaka, Masahiro Sato, Kazunori Yamahira
    • Journal Title

      JOURNAL OF EVOLUTIONARY BIOLOGY

      Volume: 34 Issue: 7 Pages: 1133-1143

    • DOI

      10.1111/jeb.13874

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] First record of Stiphodon annieae Keith &amp;amp; Hadiaty, 2014 (Teleostei, Oxudercidae) from Sulawesi Island, Indonesia2021

    • Author(s)
      Gani Abdul、Nurjirana Nurjirana、Bakri Achmad、Adriany Devita、Wuniarto Erwin、Khartiono Lady、Satria Dawam Heksa、Hasan Veryl、Herjayanto Muh.、Burhanuddin Andi Iqbal、Moore Abigail Mary、Kobayashi Hirozumi
    • Journal Title

      Check List

      Volume: 17 Issue: 1 Pages: 261-267

    • DOI

      10.15560/17.1.261

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A New “Beakless” Halfbeak of the Genus Nomorhamphus from Sulawesi (Teleostei: Zenarchopteridae)2020

    • Author(s)
      Kobayashi Hirozumi、Masengi Kawilarang W. A.、Yamahira Kazunori
    • Journal Title

      Copeia

      Volume: 108 Issue: 3 Pages: 522-531

    • DOI

      10.1643/ci-19-313

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A record of Tetraroge nigra (Tetrarogidae) from Iriomote Island, southern Japan, with notes on its ecological aspects2020

    • Author(s)
      Sato Mao、Kobayashi Hirozumi、Iida Midori、Shirai Kotaro、Sasaki Kunio
    • Journal Title

      Ichthyological Research

      Volume: 68 Issue: 1 Pages: 207-213

    • DOI

      10.1007/s10228-020-00763-5

    • NAID

      40022462175

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Latitudinal variation in sexual dimorphism in a freshwater fish group2020

    • Author(s)
      Sumarto Bayu K A、Kobayashi Hirozumi、Kakioka Ryo、Tanaka Rieko、Maeda Ken、Tran Hau D、Koizumi Noriyuki、Morioka Shinsuke、Bounsong Vongvichith、Watanabe Katsutoshi、Musikasinthorn Prachya、Tun Sein、Yun L K C、Anoop V K、Raghavan Rajeev、Masengi Kawilarang W A、Fujimoto Shingo、Yamahira Kazunori
    • Journal Title

      Biological Journal of the Linnean Society

      Volume: 131 Issue: 4 Pages: 898-908

    • DOI

      10.1093/biolinnean/blaa166

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Morphologies and population genetic structures of the eight-barbel loach of the genus Lefua on southern Sakhalin2020

    • Author(s)
      Machida Yoshiyasu、Kanaiwa Minoru、Shedko Sergey V.、Matsubara Hajime、Kobayashi Hirozumi、Mandagi Ixchel F.、Ooyagi Akira、Yamahira Kazunori
    • Journal Title

      Ichthyological Research

      Volume: 68 Issue: 2 Pages: 239-248

    • DOI

      10.1007/s10228-020-00783-1

    • NAID

      40022547174

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 琉球列島のアンキアライン洞窟におけるドウクツヌマエビの新分布記録2019

    • Author(s)
      小林大純・内田晃士・鈴木廣志・藤田喜久
    • Journal Title

      Fauna Ryukyuana

      Volume: 51 Pages: 9-12

    • NAID

      120006889913

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 琉球列島におけるホシマダラハゼ属魚類の系統および分類学的再検討2021

    • Author(s)
      小林大純・山川宇宙・佐藤真央・前田 健・山平寿智
    • Organizer
      沖縄生物学会第58回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 九州地方におけるアブラハヤ属魚類の多様性2021

    • Author(s)
      小林大純・日比野友亮
    • Organizer
      2021年度日本魚類学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 西太平洋産カワアナゴ属の分類学的再検討2021

    • Author(s)
      前田健・小林大純・山川宇宙・新里宙也・Herminie P. Palla・Hau Duc Tran
    • Organizer
      2021年度日本魚類学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Diadromous migration of gobies in Vietnam in comparison with other Asian regions.2021

    • Author(s)
      Midori Iida, Hirozumi Kobayashi, Hau Duc Tran, Ken Maeda
    • Organizer
      Annual Meeting of Ichthyological Society of Vietnam
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 同一島嶼における洞窟性魚類の異なる進化履歴2021

    • Author(s)
      小林 大純,K.W.A. Masengi, 山平 寿智
    • Organizer
      日本生態学会第68回全国大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Latitudinal Variation in Sexual Dimorphisms in a Freshwater Fish Group2021

    • Author(s)
      Sumarto B.A.K., S. Fujimoto, H. Kobayashi, K. Yamahira
    • Organizer
      日本生態学会第68回全国大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] スラウェシ島におけるヒナハゼ属魚類 “Redigobius penango” の非回遊性と系統分類 / インド太平洋における洞窟性カワアナゴ属魚類の種分化機構と適用すべき学名2021

    • Author(s)
      小林 大純
    • Organizer
      魚類分類研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Evolution and migratory ecology of widespread cave fishes in Indo-Pacific islands2019

    • Author(s)
      Kobayashi, H., Maeda, K., Shirai, K., Iida, M.and K. Yamahira
    • Organizer
      The first Annual Meeting of Ichthyological Society of Vietnam. Hanoi, (Vietnam)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phylogenomics reveals the evolution and diversity of the amphidromous fishes2019

    • Author(s)
      Kobayashi, H.
    • Organizer
      International Seminar on "Goboidei, Amphidromous Fishes" Muhammadiyah University of Luwuk Banggai, Luwuk (indonesia)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2019-05-29   Modified: 2024-03-26  

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