| Project/Area Number |
19J23043
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Review Section |
Basic Section 28040:Nanobioscience-related
|
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Morishita Kiyoshi 東京大学, 工学系研究科, 特別研究員(DC1)
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| Project Period (FY) |
2019-04-25 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2021: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2020: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2019: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Supramolecular chemistry / Colloidal Assembly / DNA nanotechnology / Nanoparticles / Proteins / GroEL / Materials Science / Colloidal assembly / Nanobiotechnology / Materials Engineering / nanoparticle / protein / DNA / supramolecular chemistry / assembly / nanomaterials |
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| Outline of Research at the Start |
This research is on the development of a gold nanoparticle platform for applications in biotechnology and materials science, such as a strategy for protein binding and manipulation. This work will also reveal fundamental insights into the interactions between engineered nanoparticles and proteins.
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| Outline of Annual Research Achievements |
This year I worked on i) porous clusters formed by GroEL and gold nanoparticles (AuNPs), and ii) rugby ball self-assembly of DNA-functionalized GroEL.
In project i) I confirmed the ATPase activity of the clusters and analyzed the porous nature of the clusters by TEM, cryo TEM, and cryo-EM tomography. This quantitative approach confirmed that the clusters had discrete numbers of GroEL connecting AuNPs, as designed. The porous nature was also probed by guest loading studies, using various polymer-protein hybrids as guest species. This indicated that the cluster can accommodate guests on the order of several tens of nanometers in size, with a guest hydrodynamic diameter limit of around 45 nm. Experiments on the effect of guest polymer conformation on loading are ongoing.
In project ii) I studied the roles of various nucleotides and analogues, DNA sequence, and salt conditions in the rugby ball formation. This gave a complete understanding of the important factors for growth and supported a proposed mechanism for the assembly. I identified the alignment of GroEL units in the structure and its anisotropic nature. Current experiments are targeted to identify whether the structure is hollow.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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