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Development of fuctional molecular recognition elements utilizing a highly stable cushioning scaffold protein

Research Project

Project/Area Number 19K05166
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionOkayama University

Principal Investigator

IMANAKA Hiroyuki  岡山大学, 自然科学学域, 助教 (10379711)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsバイオセンサー / 生体分子間相互作用 / 足場タンパク質 / 表面親和性ペプチド / 固定化 / 分子認識素子 / バイオ分子 / 相互作用 / 蛋白質 / ペプチド / リガンド / 超好熱菌 / 足場 / タンパク質 / 生体分子 / 分子デザイン / タンパク質工学 / 分子間相互作用
Outline of Research at the Start

本研究では構造が極めて安定な超好熱菌由来三量体タンパク質CutA1を,リガンドバイオ分子(ペプチドあるいはタンパク質)提示のためのクッション性足場として利用し,バイオ分子間相互作用を制御・検出する技術の基盤要素の一つである分子認識素子を独自のアイデアに基づき緻密に設計する.詳細な機能評価を通じ,分子認識素子の配向制御,機能維持ならびに多価化を同時に担保しうる分子設計・調製技術の基盤を確立する.さらに,進化分子工学的手法等を駆使し,標的バイオ分子との相互作用の高感度検出・精密制御を可能とする分子認識素子を新たに創出する.

Outline of Final Research Achievements

In order to establish a simple and rapid biosensing system for detection of biomolecular interactions on solid substrates, we attempted to expand the molecular design diversity of CutA1, a novel scaffold protein, and verified its function. An objective multivalent CutA1 was successfully developed by identifying a novel sequence insertion site. In addition, single-chained CutA1, for another type of multivalent molecular recognition element, was also constructed by the insertion of appropriate linker sequences between CutA1 subunits. As for the detection of biomolecular interactions, the detection sensitivity can be improved by controlling the arrangement and orientation of the ligand molecules and by introducing irregularities at the nanobio-interface.

Academic Significance and Societal Importance of the Research Achievements

バイオセンサーへの適用に向け,簡便な調製が可能で,構造が極めて安定な超好熱菌由来タンパク質CutA1を用い,標的分子認識素子(ペプチドあるいはタンパク質)を提示するための足場として利用する新しいバイオ分子間相互作用制御システムの技術基盤を確立した.本研究の成果により,独自のアイデアに基づき,医療(診断)・創薬・食品・環境分野といった幅広い分野で適用可能な,多様で高感度なバイオセンサーの開発が期待できる.

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (12 results)

All 2021 2020 2019

All Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Presentation] Design of functional nano-bio interfaces using CutA1 as a proteinaceous scaffold2021

    • Author(s)
      Hiroyuki Imanaka
    • Organizer
      2021 KSIEC Spring Meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Characterization of VHH-EGFP interaction for the construction of functional molecular recognition elements2021

    • Author(s)
      Imanaka, H., Y. Ikeda, N. Ishida and K. Imamura
    • Organizer
      第21回日本蛋白質科学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Design of functional nano-bio interfaces using CutA1 as a proteinaceous scaffold2021

    • Author(s)
      Hiroyuki Imanaka
    • Organizer
      2021 Fall meeting of the KSBM
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 自発的アセンブリを介した多価化CutA1の分子デザイン2021

    • Author(s)
      山田航大,石田尚之,今村維克,今中洋行
    • Organizer
      化学工学会第52回秋季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] CutA1とタンパク質性分子糊を利用した機能性ナノバイオ界面の設計2021

    • Author(s)
      黒瀬志帆,石田尚之,今村維克,今中洋行
    • Organizer
      2021年度日本農芸化学会 西日本・中四国・関西支部 合同大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Development and Aplication of Multivalent CutA12021

    • Author(s)
      Imanaka, H., H. Azuma, S. Kurose and K. Imamura
    • Organizer
      The 26th Symposium of YABEC
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規多価化分子認識素子の創出に向けた一本鎖型CutA1量体構造改変の試み2020

    • Author(s)
      山田航大,東秀隆,石田尚之,今村維克,今中洋行
    • Organizer
      日本生物工学会西日本支部大会2020
    • Related Report
      2020 Research-status Report
  • [Presentation] CutA1とBarnase-Barstar間相互作用を利用したナノバイオ界面デザイン2020

    • Author(s)
      黒瀬志帆,石田尚之,今村維克,今中洋行
    • Organizer
      日本生物工学会西日本支部大会2020
    • Related Report
      2020 Research-status Report
  • [Presentation] CutA1量体構造改変による新規多価化分子認識素子の創出2020

    • Author(s)
      山田航大,東秀隆,石田尚之,今村維克,今中洋行
    • Organizer
      第22回化学工学会学生発表会
    • Related Report
      2019 Research-status Report
  • [Presentation] 分子認識における多点相互作用の検証とタンパク性足場分子の機能開発2020

    • Author(s)
      今中 洋行・池田 湧・黒瀬 志帆・東 秀隆・石田 尚之・今村 維克
    • Organizer
      化学工学会第85年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Functionalization of AuNPs by one-step ligand immobilization with designer scaffold protein and its application for sensitive colorimetric interaction detection2019

    • Author(s)
      Hiroyuki Imanaka, Yu Ikeda, Haruka Matoba, Naoyuki Ishida and Koreyoshi Imamura
    • Organizer
      Asian Congress on Biotechnology 2019 (ACB2019)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] VHH抗体提示型分子認識素子を用いたAuNPのワンステップ機能化2019

    • Author(s)
      池田 湧, 石田 尚之, 今村 維克, 今中 洋行
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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