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Uranyl binding peptide for nanopore sequencing

Research Project

Project/Area Number 19K05325
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 31010:Nuclear engineering-related
Research InstitutionTokyo Institute of Technology

Principal Investigator

Tsushima Satoru  東京工業大学, 科学技術創成研究院, 特任准教授 (80312990)

Co-Investigator(Kenkyū-buntansha) 鷹尾 康一朗  東京工業大学, 科学技術創成研究院, 准教授 (00431990)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsウラニルイオン / ペプチド / ウラン / 計算化学 / MD計算 / FMO計算 / アミノ酸 / 光化学
Outline of Research at the Start

近年、対象とする生体分子をナノ孔に通して、その相互作用で発生する微小電流を観測することによって生体分子の塩基やアミノ酸の配列を決めるナノポアシーケンサーの分野の発展が目覚ましい。本研究においては、工学的に設計したペプチドに溶液中のウラニルイオンを選択的に捕捉させ、この溶液をナノポアシーケンサーに通すことで、地下水や飲料水中のウラン含有量を簡便に測定する手法を開発することを目的とする。ウランとペプチドの相互作用に関する学術的に新しい知見を得るとともに、それを即座に応用に繋げることが本研究の目標とするところである。本研究では、計算化学的手法を分子設計に採り入れることも大きな特色とする。

Outline of Final Research Achievements

Cyclic peptides as well as a modified EF-hand motif of calmodulin have been newly designed to achieve high affinity towards uranyl(VI). Cyclic peptides may be engineered to bind uranyl(VI) to its backbone under acidic conditions, which may enhance its selectivity. For the modified EF-hand motif of calmodulin, strong electrostatic interactions between uranyl(VI) and negatively charged side chains play an important role in achieving high affinity; however, it is also essential to have a secondary structure element and formation of hydrophobic cores in the metal-bound state of the peptide.

Academic Significance and Societal Importance of the Research Achievements

本研究により、ウラニルイオンに選択的なペプチドを新たに開発することができた。この成果によりウランとペプチドの分子レベルでの相互作用を系統的に明らかにすることができた上、ウランと生体物質の相互作用の礎となる知識を新たに蓄積できた。この知見を学界で広く共有することにより、この分野の新たな展開と発展への学術的貢献が実現できると考える。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (6 results)

All 2022 2021 2020 Other

All Int'l Joint Research (3 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (1 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] Helmholtz-Zentrum Dresden-Rossendorf(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] HZDR(ドイツ)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] HZDR(ドイツ)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Hydrophobic core formation and secondary structure elements in uranyl(VI)-binding peptides2022

    • Author(s)
      Satoru Tsushima, Koichiro Takao
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 24 Issue: 7 Pages: 4455-4461

    • DOI

      10.1039/d1cp05401e

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Crystallization of colourless hexanitratoneptunate(IV) with anhydrous H+ countercations trapped in a hydrogen bonded polymer with diamide linkers†2020

    • Author(s)
      Koichiro Takao, Juliane Maerz, Moe Matsuoka, Takanori Mashita, Hiroyuki Kazama, Satoru Tsushima
    • Journal Title

      RSC Advances

      Volume: 10 Issue: 10 Pages: 6082-6087

    • DOI

      10.1039/c9ra10090c

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Selective binding of UO2 2+ by different peptide families2021

    • Author(s)
      Satoru Tsushima
    • Organizer
      PACIFICHEM 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

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