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Water-in-oil method for single-cell proteomics

Research Project

Project/Area Number 19K05544
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 34020:Analytical chemistry-related
Research InstitutionKumamoto University

Principal Investigator

Masuda Takeshi  熊本大学, 大学院生命科学研究部(薬), 助教 (70383940)

Co-Investigator(Kenkyū-buntansha) 宇都 甲一郎  国立研究開発法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, 独立研究者 (30597034)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsプロテオミクス / 高感度分析 / 1細胞オミクス / 油中液滴
Outline of Research at the Start

1細胞から1,000種類以上のタンパク質をハイスループットに定性・定量できる分析システムを開発する。1細胞プロテオミクスを達成することで、同一細胞群内における分子レベルの不均一性発生機構や不均一性が生み出す発生や分化に関わる複雑な生命機構の解明につながる。これまでにない分解能および視点から生命活動を観察できる。目的を達成するために、申請者が独自開発した油中液滴チャンバー法を基盤とする。

Outline of Final Research Achievements

In this project, we aim to establish a practical single-cell proteomics system. This study was based on the water droplet-in-oil digestion (WinO) method which was established by the applicant. We developed a pseudo amplification method for proteins using stable isotope-labeled reagents. To achieve high throughput analysis, we established a semi-automated pretreatment using a liquid handling robot. These improvements dramatically increased the number of quantified proteins in single-cell proteomics. We also developed a superhydrophobic materials suitable for the WinO method.

Academic Significance and Societal Importance of the Research Achievements

細胞の分子レベルの不均一性は、組織や器官の複雑な機能やガン細胞集団としての抗ガン剤耐性を生み出すなど、重要な生命現象に深く関与している。本申請課題では、これまで難しかった1細胞プロテオミクスの実現に大きく近づける基盤技術を構築した。1細胞プロテオミクスを実現することで、従来のプロテオミクスでは見落としていた新たな現象の発見につながることで、学術的意義が大きい成果と言える。また、1細胞プロテオミクス技術は新規治療標的の探索などへの応用にも期待できることから、本成果は社会的意義もある。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (49 results)

All 2022 2021 2020 2019

All Journal Article (27 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 26 results,  Open Access: 9 results) Presentation (22 results) (of which Invited: 2 results)

  • [Journal Article] Trends in Single-Cell Proteomics Technology2022

    • Author(s)
      Masuda Takeshi
    • Journal Title

      Journal of the Mass Spectrometry Society of Japan

      Volume: 70 Issue: 1 Pages: 72-73

    • DOI

      10.5702/massspec.S22-13

    • ISSN
      1340-8097, 1880-4225, 1884-3271
    • Year and Date
      2022-03-01
    • Related Report
      2021 Annual Research Report
  • [Journal Article] Objective discrimination of fur type based on electrophoresis optimized for fur‐hair proteins2022

    • Author(s)
      Sekimoto Yuri、Tokai Arisa、Aritaki Muneshige、Ando Takeshi、Masuda Takeshi
    • Journal Title

      ELECTROPHORESIS

      Volume: - Issue: 11 Pages: 1233-1241

    • DOI

      10.1002/elps.202200002

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Knockdown of Podocalyxin Post-Transcriptionally Induces the Expression and Activity of ABCB1/MDR1 in Human Brain Microvascular Endothelial Cells2022

    • Author(s)
      Nagano Hinako、Ogata Seiryo、Ito Shingo、Masuda Takeshi、Ohtsuki Sumio
    • Journal Title

      Journal of Pharmaceutical Sciences

      Volume: - Issue: 6 Pages: 1812-1819

    • DOI

      10.1016/j.xphs.2022.02.006

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomics Analysis of Lymphatic Metastasis-Related Proteins Using Highly Metastatic Human Melanoma Cells Originated by Sequential <i>in Vivo</i> Implantation2021

    • Author(s)
      Chida Katsuyuki、Sakurai Yu、Ohtani Asa、Masuda Takeshi、Ohtsuki Sumio、Tanaka Hiroki、Akita Hidetaka
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 44 Issue: 10 Pages: 1551-1556

    • DOI

      10.1248/bpb.b21-00463

    • NAID

      130008095758

    • ISSN
      0918-6158, 1347-5215
    • Year and Date
      2021-10-01
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Water droplet-in-oil digestion method for single-cell proteomics2021

    • Author(s)
      Masuda Takeshi、Inamori Yuma、Furukawa Arisu、Momosaki Kazuki、Chang Chih-Hsiang、Kobayashi Daiki、Ohguchi Hiroto、Kawano Yawara、Ito Shingo、Araki Norie、Ong Shao-En、Ohtsuki Sumio
    • Journal Title

      bioRxiv

      Volume: - Pages: 432378-432378

    • DOI

      10.1101/2021.12.13.472378

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of Insulin Receptor-Knockdown on the Expression Levels of Blood?Brain Barrier Functional Proteins in Human Brain Microvascular Endothelial Cells2021

    • Author(s)
      Nagano Hinako、Ito Shingo、Masuda Takeshi、Ohtsuki Sumio
    • Journal Title

      Pharmaceutical Research

      Volume: - Issue: 7 Pages: 1561-1574

    • DOI

      10.1007/s11095-021-03131-8

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acetylation of the influenza A virus polymerase subunit PA in the N‐terminal domain positively regulates its endonuclease activity2021

    • Author(s)
      Hatakeyama Dai、Shoji Masaki、Ogata Seiryo、Masuda Takeshi、Nakano Masahiro、Komatsu Tsugunori、Saitoh Ayaka、Makiyama Kyoko、Tsuneishi Hazuki、Miyatake Asuka、Takahira Mizuki、Nishikawa Erina、Ohkubo Ayana、Noda Takeshi、Kawaoka Yoshihiro、Ohtsuki Sumio、Kuzuhara Takashi
    • Journal Title

      The FEBS Journal

      Volume: 289 Issue: 1 Pages: 231-245

    • DOI

      10.1111/febs.16123

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lysine Demethylase 5A is Required for MYC Driven Transcription in Multiple Myeloma2021

    • Author(s)
      Ohguchi H、Park Paul M.C.、wang t、Gryder B E.、Ogiya D、kurata k、zhang x、Li D、pei c、Masuda T、Johansson C、Wimalasena V K、Kim Y、Hino S、Usuki S、Kawano Y、Samur M K、Tai Yu-Tzu、Munshi N C.、Matsuoka M、Ohtsuki S、Nakao M、Minami T、Lauberth S、Khan J、Oppermann U、Durbin A D.、Anderson K C.、Hideshima T、Qi J
    • Journal Title

      Blood Cancer Discovery

      Volume: in press Issue: 4 Pages: 370-387

    • DOI

      10.1158/2643-3230.bcd-20-0108

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In-vitro acetylation of SARS-CoV and SARS-CoV-2 nucleocapsid proteins by human PCAF and GCN52021

    • Author(s)
      Hatakeyama Dai、Masuda Takeshi、Miki Ryosuke、Ohtsuki Sumio、Kuzuhara Takashi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 557 Pages: 273-279

    • DOI

      10.1016/j.bbrc.2021.03.173

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oral Coadministration of Zn-Insulin with d-Form Small Intestine-Permeable Cyclic Peptide Enhances Its Blood Glucose-Lowering Effect in Mice2021

    • Author(s)
      Ito Shingo、Torii Yuta、Chikamatsu Shoma、Harada Tomonori、Yamaguchi Shunsuke、Ogata Seiryo、Sonoda Kayoko、Wakayama Tomohiko、Masuda Takeshi、Ohtsuki Sumio
    • Journal Title

      Molecular Pharmaceutics

      Volume: 18 Issue: 4 Pages: 1593-1603

    • DOI

      10.1021/acs.molpharmaceut.0c01010

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantitative and targeted proteomics-based identification and validation of drug efficacy biomarkers2021

    • Author(s)
      Masuda Takeshi、Mori Ayano、Ito Shingo、Ohtsuki Sumio
    • Journal Title

      Drug Metabolism and Pharmacokinetics

      Volume: 36 Pages: 100361-100361

    • DOI

      10.1016/j.dmpk.2020.09.006

    • NAID

      210000165083

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Efficient isolation of brain capillary from a single frozen mouse brain for protein expression analysis2020

    • Author(s)
      Ogata Seiryo、Ito Shingo、Masuda Takeshi、Ohtsuki Sumio
    • Journal Title

      Journal of Cerebral Blood Flow & Metabolism

      Volume: 271678 Issue: 5 Pages: 1026-1038

    • DOI

      10.1177/0271678x20941449

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] SHOC2 Is a Critical Modulator of Sensitivity to EGFR-TKIs in Non-Small Cell Lung Cancer Cells2020

    • Author(s)
      Terai Hideki、Hamamoto Junko、Emoto Katsura、Masuda Takeshi、Manabe Tadashi、Kuronuma Satoshi、Kobayashi Keigo、Masuzawa Keita、Ikemura Shinnosuke、Nakayama Sohei、Kawada Ichiro、Suzuki Yusuke、Takeuchi Osamu、Suzuki Yukio、Ohtsuki Sumio、Yasuda Hiroyuki、Soejima Kenzo、Fukunaga Koichi
    • Journal Title

      Molecular Cancer Research

      Volume: 19 Issue: 2 Pages: 317-328

    • DOI

      10.1158/1541-7786.mcr-20-0664

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Proteomic Evaluation of Plasma Membrane Fraction Prepared from a Mouse Liver and Kidney Using a Bead Homogenizer: Enrichment of Drug-Related Transporter Proteins2020

    • Author(s)
      Yagi Ryotaro、Masuda Takeshi、Ogata Seiryo、Mori Ayano、Ito Shingo、Ohtsuki Sumio
    • Journal Title

      Molecular Pharmaceutics

      Volume: 17 Issue: 11 Pages: 4101-4113

    • DOI

      10.1021/acs.molpharmaceut.0c00547

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Convenient method of producing cyclic single-chain Fv antibodies by split-intein-mediated protein ligation and chaperone co-expression2020

    • Author(s)
      Liu Chenjiang、Kobashigawa Yoshihiro、Yamauchi Soichiro、Fukuda Natsuki、Sato Takashi、Masuda Takeshi、Ohtsuki Sumio、Morioka Hiroshi
    • Journal Title

      The Journal of Biochemistry

      Volume: 168 Issue: 3 Pages: 257-263

    • DOI

      10.1093/jb/mvaa042

    • NAID

      40022375418

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of differences in pre-analytical processing on blood protein profiles determined with SWATH-MS2020

    • Author(s)
      Nambu Madoka、Nishiumi Shin、Kobayashi Takashi、Masuda Takeshi、Ito Shingo、Yoshida Masaru、Ohtsuki Sumio
    • Journal Title

      Journal of Proteomics

      Volume: 223 Pages: 103824-103824

    • DOI

      10.1016/j.jprot.2020.103824

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nicotine promotes angiogenesis in mouse brain after intracerebral hemorrhage2020

    • Author(s)
      Matsumoto Kosei、Kinoshita Keita、Hijioka Masanori、Kurauchi Yuki、Hisatsune Akinori、Seki Takahiro、Masuda Takeshi、Ohtsuki Sumio、Katsuki Hiroshi
    • Journal Title

      Neuroscience Research

      Volume: - Pages: 284-294

    • DOI

      10.1016/j.neures.2020.07.003

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Laminin Subunit Alpha-4 and Osteopontin Are Glioblastoma-Selective Secreted Proteins That Are Increased in the Cerebrospinal Fluid of Glioblastoma Patients2020

    • Author(s)
      Kohata Tomohiro、Ito Shingo、Masuda Takeshi、Furuta Takuya、Nakada Mitsutoshi、Ohtsuki Sumio
    • Journal Title

      Journal of Proteome Research

      Volume: 19 Issue: 8 Pages: 3542-3553

    • DOI

      10.1021/acs.jproteome.0c00415

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Cell-Surface Proteins Endocytosed by Human Brain Microvascular Endothelial Cells In Vitro2020

    • Author(s)
      Ito Shingo、Oishi Mariko、Ogata Seiryo、Uemura Tatsuki、Couraud Pierre-Olivier、Masuda Takeshi、Ohtsuki Sumio
    • Journal Title

      Pharmaceutics

      Volume: 12 Issue: 6 Pages: 579-579

    • DOI

      10.3390/pharmaceutics12060579

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Novel cyclic peptides facilitating transcellular blood-brain barrier transport of macromolecules in vitro and in vivo2020

    • Author(s)
      1.Shunsuke Yamaguchi, Shingo Ito, Takeshi Masuda, Pierre-Olivier Couraud, Sumio Ohtsuki
    • Journal Title

      J Control Release

      Volume: 321 Pages: 744-755

    • DOI

      10.1016/j.jconrel.2020.03.001

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cyclocreatine Transport by SLC6A8, the Creatine Transporter, in HEK293 Cells, a Human Blood-Brain Barrier Model Cell, and CCDSs Patient-Derived Fibroblasts.2020

    • Author(s)
      Uemura T, Ito S, Masuda T, Shimbo H, Goto T, Osaka H, Wada T, Couraud PO, Ohtsuki S.
    • Journal Title

      Pharm Res.

      Volume: 37 Issue: 3 Pages: 1-11

    • DOI

      10.1007/s11095-020-2779-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Leucine-Rich Alpha-2-Glycoprotein 1 in Serum Is a Possible Biomarker to Predict Response to Preoperative Chemoradiotherapy for Esophageal Cancer2019

    • Author(s)
      Nambu Madoka、Masuda Takeshi、Ito Shingo、Kato Ken、Kojima Takashi、Daiko Hiroyuki、Ito Yoshinori、Honda Kazufumi、Ohtsuki Sumio
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 42 Issue: 10 Pages: 1766-1771

    • DOI

      10.1248/bpb.b19-00395

    • NAID

      130007722442

    • ISSN
      0918-6158, 1347-5215
    • Year and Date
      2019-10-01
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mass Spectrometry-Compatible Subcellular Fractionation for Proteomics2019

    • Author(s)
      Masuda Takeshi、Sugiyama Naoyuki、Tomita Masaru、Ohtsuki Sumio、Ishihama Yasushi
    • Journal Title

      Journal of Proteome Research

      Volume: 19 Issue: 1 Pages: 75-84

    • DOI

      10.1021/acs.jproteome.9b00347

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Changes of Blood-Brain Barrier and Brain Parenchymal Protein Expression Levels of Mice under Different Insulin-Resistance Conditions Induced by High-Fat Diet.2019

    • Author(s)
      Ogata S, Ito S, Masuda T, Ohtsuki S
    • Journal Title

      Pharm Res

      Volume: 36 Issue: 10 Pages: 141-141

    • DOI

      10.1007/s11095-019-2674-8

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of a lipoplex-type mRNA carrier composed of an ionizable lipid with a vitamin E scaffold and the KALA peptide for use as an ex vivo dendritic cell-based cancer vaccine2019

    • Author(s)
      Tateshita N, Miura N, Tanaka H, Masuda T, Ohtsuki S, Tange K, Nakai Y, Yoshioka H, Akita H
    • Journal Title

      Journal of Controlled Release

      Volume: 310 Pages: 36-46

    • DOI

      10.1016/j.jconrel.2019.08.002

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Large-Scale Quantitative Comparison of Plasma Transmembrane Proteins between Two Human Blood-Brain Barrier Model Cell Lines, hCMEC/D3 and HBMEC/cibeta.2019

    • Author(s)
      Masuda T, Hoshiyama T, Uemura T, Hirayama-Kurogi M, Ogata S, Furukawa A, Couraud PO, Furihata T, Ito S, Ohtsuki S
    • Journal Title

      Mol Pharm

      Volume: 16 Issue: 5 Pages: 2162-2171

    • DOI

      10.1021/acs.molpharmaceut.9b00114

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cyclization of Single-Chain Fv Antibodies Markedly Suppressed Their Characteristic Aggregation Mediated by Inter-Chain VH-VL Interactions2019

    • Author(s)
      Yamauchi Soichiro、Kobashigawa Yoshihiro、Fukuda Natsuki、Teramoto Manaka、Toyota Yuya、Liu Chenjiang、Ikeguchi Yuka、Sato Takashi、Sato Yuko、Kimura Hiroshi、Masuda Takeshi、Ohtsuki Sumio、Noi Kentaro、Ogura Teru、Morioka Hiroshi
    • Journal Title

      Molecules

      Volume: 24 Issue: 14 Pages: 2620-2620

    • DOI

      10.3390/molecules24142620

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 油中液滴法を基盤とした1細胞プロテオミクス2022

    • Author(s)
      増田豪
    • Organizer
      第19回北里疾患プロテオーム研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 油中液滴法が可能にする1細胞プロテオミクス2022

    • Author(s)
      増田豪
    • Organizer
      細胞社会ダイバーシティーの統合的解明と制御;回若手ワークショップ
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 定量的標的プロテオミクスによるヒト肝トランスポーターのタンパク質定量値の評価2021

    • Author(s)
      森彩乃, 増田豪, 伊藤慎悟, 大槻純男
    • Organizer
      日本薬物動態学会 第36回年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Appノックイン型アルツハイマー病モデルマウス肝臓における薬物代謝・輸送関連タンパク質発現変動の解明2021

    • Author(s)
      岩田優実, 伊藤慎悟, 金子由依, 緒方星陵, 齊藤貴志, 西道隆臣, 増田豪, 大槻純男
    • Organizer
      日本薬物動態学会 第36回年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Cyclocreatine transport by creatine transporter (SLC6A8) in HEK293 cells, a human blood-brain barrier model cell, and CCDSs patient-derived fibroblasts2021

    • Author(s)
      Shingo Ito, Tatsuki Uemura, Takeshi Masuda, Hiroko Shimbo, Tomohide Goto, Hitoshi Osaka, Takahito Wada, Pierre-Olivier Couraud, Sumio Ohtsuki
    • Organizer
      36th JSSX Annual Meeting
    • Related Report
      2021 Annual Research Report
  • [Presentation] 定量プロテオーム解析による新規不死化ヒトリンパ管内皮細胞の評価2021

    • Author(s)
      古西修希, 櫻井遊, 千田克幸, 増田豪, 伊藤慎悟, 降幡知巳, 秋田英万, 大槻純男
    • Organizer
      第38回日本薬学会九州山口支部大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 小腸透過環状ペプチド共投与法を用いたインスリンの経口投与法の改良2021

    • Author(s)
      近松翔馬, 伊藤慎悟, 増田豪, 大槻純男
    • Organizer
      第38回日本薬学会九州山口支部大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 近松翔馬, 伊藤慎悟, 増田豪, 大槻純男2021

    • Author(s)
      Improvement of insulin absorption by oral co-administration with small intestine-permeable cyclic peptide concatenated with an insulin-binding peptide
    • Organizer
      第15回次世代を担う若手のための医療薬科学シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] 1細胞プロテオミクスを目指した技術開発2021

    • Author(s)
      増田豪
    • Organizer
      BioJapan 2021
    • Related Report
      2021 Annual Research Report
  • [Presentation] 膜タンパク質の発現量を網羅的かつ正しく俯瞰するには2021

    • Author(s)
      増田豪
    • Organizer
      第12回 トランスポーター研究会 九州部会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 標的プロテオミクスによる膜タンパク質定量のペプチド分画による高感度化2021

    • Author(s)
      宮野綾華, 森彩乃, 増田豪, 伊藤慎悟, 大槻純男
    • Organizer
      日本プロテオーム学会2021年大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 細胞膜タンパク質ビオチン標識法とSWATH-MSによる網羅的タンパク質定量法を組み合わせたヒト脳毛細血管内皮細胞における細胞内内在化膜タンパク質の同定2021

    • Author(s)
      伊藤慎悟, 大石真梨子, 緖方星陵, 上村立記, Pierre-Olivier Couraud, 増田豪, 大槻純男
    • Organizer
      第37回日本DDS学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] マウス大脳と小脳の単離毛細血管における血液脳関門プロテオーム比較解析2021

    • Author(s)
      吉田尚功, 伊藤慎悟, 緒方星陵, 増田豪, 大槻純男
    • Organizer
      日本薬剤学会第36年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 高感度・高精度なプロテオミクス技術2021

    • Author(s)
      増田豪
    • Organizer
      日本薬学会第141年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 油中液滴法を用いた100細胞プロテオミクスの有効性2020

    • Author(s)
      稲森悠真、増田豪、伊藤慎悟、大槻純男
    • Organizer
      日本薬物動態学会第35年会
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Published: 2019-04-18   Modified: 2023-01-30  

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