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Development of ultra-high resolution electrochemical bioimaging method to elucidate intercellular communication

Research Project

Project/Area Number 19K05687
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 37010:Bio-related chemistry
Research InstitutionUniversity of Yamanashi (2020-2021)
Tohoku University (2019)

Principal Investigator

Kumi Inoue  山梨大学, 大学院総合研究部, 准教授 (20597249)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsバイオイメージング / バイポーラ電気化学 / 化学顕微鏡 / 電極アレイ / 電気化学センサ / 細胞間コミュニケーション / 電気化学顕微鏡 / バイポーラ電極 / 微小電極
Outline of Research at the Start

細胞間コミュニケーションの解明には、細胞近傍の局所領域で動的に変化を続ける化学情報を定量的に観察できる手段が必要である。細胞本来の活動を乱さずに観察するためには非染色・低侵襲に計測できる技術が必要であり、微小電極を用いる電気化学イメージング技術が期待されているが、必要な解像度と速度を併せ持つ技術の完成には未だ至っていない。そこで本研究では各電極への導線を必要としない「微細バイポーラ電極アレイ」を利用することで、高解像度かつ高速の電気化学イメージングを実現し、神経細胞からのドパミン放出のような単一細胞レベルの生体機能イメージングを行える革新的な観察プラットフォームの構築を行う。

Outline of Final Research Achievements

To elucidate cell-cell communication, a chemical microscopy is required to quantitatively observe chemical information that changes dynamically in a local region near the cell. An electrochemical imaging technique using microelectrode is expected to observe the activity of cells with minimal invasion. However, conventional electrochemical microscopy has a trade-off between temporal resolution and spatial resolution. In this study, we realized high-resolution and high-speed electrochemical imaging by using a micro bipolar electrode array that does not require a lead wire to each electrode. We also demonstrated biological activity imaging including cell respiration imaging and dopamine imaging using this platform.

Academic Significance and Societal Importance of the Research Achievements

本研究では、生命システムの解明ツールとして利用可能な動的化学イメージング技術として、新たにバイポーラ電気化学顕微鏡の原理を確立し、その応用可能性を示した。本原理は、従来の電気化学顕微鏡の課題であった時間分解能と空間分解能の両立にブレークスルーをもたらすもので、ラベルフリーに直接的かつリアリタイムに1細胞レベルの局所領域の化学情報を可視化することができるため、細胞間の化学反応ネットワーク解明などが可能な新たな顕微鏡システムを提供できる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (50 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (1 results) Journal Article (10 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (33 results) (of which Int'l Joint Research: 16 results,  Invited: 4 results) Book (1 results) Remarks (5 results)

  • [Int'l Joint Research] EPFL(スイス)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] カソーディックルミノフォアを用いるバイポーラ電気化学顕微鏡によるドーパミン検出2022

    • Author(s)
      井上(安田) 久美、小松 万葉、岩間 智紀、珠玖 仁
    • Journal Title

      Chemical Sensors

      Volume: 38 Suppl. A Pages: 85-87

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Electrochemiluminescence imaging based on bipolar electrochemistry using commercially available anisotropic conductive films2022

    • Author(s)
      Rise Akasaka, Kosuke Ino*, Tomoki Iwama, Kumi Y. Inoue, Yuji Nashimoto, Hitoshi Shiku*
    • Journal Title

      Sensors and Materials

      Volume: 35 Pages: 0-1

    • Related Report
      2021 Annual Research Report
  • [Journal Article] That’s the Thing in Electroanalytical Chemistry! (Part 8)2021

    • Author(s)
      Yamamoto (ed) Masahiro
    • Journal Title

      Review of Polarography

      Volume: 67 Issue: 2 Pages: 83-84

    • DOI

      10.5189/revpolarography.67.83

    • NAID

      130008109028

    • ISSN
      0034-6691, 1884-7692
    • Year and Date
      2021-10-28
    • Related Report
      2021 Annual Research Report
  • [Journal Article] Thermally‐Drawn Multi‐Electrode Fibers for Bipolar Electrochemistry and Magnified Electrochemical Imaging2021

    • Author(s)
      Iwama Tomoki、Guo Yuanyuan、Handa Shoma、Y. Inoue Kumi、Yoshinobu Tatsuo、Sorin Fabien、Shiku Hitoshi
    • Journal Title

      Advanced Materials Technologies

      Volume: 7 Issue: 5 Pages: 2101066-2101066

    • DOI

      10.1002/admt.202101066

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Recent Advances in Electrochemiluminescence-Based Systems for Mammalian Cell Analysis2020

    • Author(s)
      Hiramoto Kaoru、Villani Elena、Iwama Tomoki、Komatsu Keika、Inagi Shinsuke、Inoue Kumi、Nashimoto Yuji、Ino Kosuke、Shiku Hitoshi
    • Journal Title

      Micromachines

      Volume: 11 Issue: 5 Pages: 530-530

    • DOI

      10.3390/mi11050530

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Bioimaging using bipolar electrochemical microscopy with improved spatial resolution2020

    • Author(s)
      Iwama Tomoki、Inoue Kumi Y.、Abe Hiroya、Matsue Tomokazu、Shiku Hitoshi
    • Journal Title

      The Analyst

      Volume: 145 Issue: 21 Pages: 6895-6900

    • DOI

      10.1039/d0an00912a

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biosensors and biosensing in the era of digital health2020

    • Author(s)
      INOUE Kumi、OISHI Tetsuo
    • Journal Title

      Denki Kagaku

      Volume: 88 Issue: 4 Pages: 298-298

    • DOI

      10.5796/denkikagaku.20-fe0026

    • NAID

      130007950674

    • Related Report
      2020 Research-status Report
  • [Journal Article] バイポーラ電気化学顕微鏡システムの開発と高時空間分解能バイオイメージング応用の検討2020

    • Author(s)
      岩間智紀、井上(安田)久美、阿部博弥、末永智一、珠玖 仁
    • Journal Title

      Chemical Sensors

      Volume: 34 Suppl. A Pages: 58-60

    • Related Report
      2019 Research-status Report
  • [Journal Article] Research of electrochemical sensor for highly sensitive and quantitative analysis with simple operation2019

    • Author(s)
      井上 (安田) 久美
    • Journal Title

      Review of Polarography

      Volume: 65 Issue: 1 Pages: 3-10

    • DOI

      10.5189/revpolarography.65.3

    • NAID

      130007659702

    • ISSN
      0034-6691, 1884-7692
    • Year and Date
      2019-05-23
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Real-time imaging of photosynthetic oxygen evolution from spinach using LSI-based biosensor2019

    • Author(s)
      Shigenobu Kasai, Yamato Sugiura, Ankush Prasad, Kumi Y. Inoue, Teruya Sato, Tomohiro Honmo, Aditya Kumar, Pavel Pospisil, Kosuke Ino, Yuka Hashi, Yoko Furubayashi, Masahki Matsudaira, Atsushi Suda, Ryota Kunikata and Tomokazu Matsue
    • Journal Title

      Sci. Rep

      Volume: 9 Issue: 1

    • DOI

      10.1038/s41598-019-48561-y

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ソーディックルミノフォアを用いるバイポーラ電気化学顕微鏡によるドーパミン検出2022

    • Author(s)
      井上(安田) 久美、小松 万葉、岩間 智紀、珠玖 仁
    • Organizer
      2022年 電気化学会第89回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Demonstration of magnified imaging using thermally tapered bipolar electrode array2021

    • Author(s)
      Tomoki Iwama, Yuanyuan Guo, Shoma Handa, Kumi Y. Inoue, Tatsuo Yoshinobu, Fabien Sorin, Hitoshi Shiku
    • Organizer
      72nd Annual Meeting of the International Society of Electrochemistry
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High spatio-temporal resolution electrochemical imaging system based on bipolar electrochemistry and electrochemiluminescence2021

    • Author(s)
      Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      Pacifichem 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ジメチルペリレンテトラカルボキシジイミドをcathodic電気化学発光基質に用いるバイポーラ電極系によるドーパミン検出および二次元イメージング2021

    • Author(s)
      小松万葉,岩間智紀,井上(安田)久美,伊野浩介,珠玖仁
    • Organizer
      2021年電気化学秋季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Dopamine imaging using cathodic electrochemiluminescence generated by co-reaction of ruthenium bipyridine complex and oxidized glutathione on closed bipolar electrode array2021

    • Author(s)
      Kumi. Y. Inoue, Mayo Komatsu, Tomoki Iwama, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      72nd Annual ISE Meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ルテニウムビピリジン錯体と酸化型グルタチオンの共反応による陰極電気化学発光を用いたclosedバイポーラ電極システムによるモノアミン検出および生体分子イメージングに向けた検討2021

    • Author(s)
      小松万葉,岩間智紀,井上(安田)久美,伊野浩介,珠玖仁
    • Organizer
      電気化学会第88回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 高時空間分解能バイオイメージングに向けたバイポーラ電気化学顕微鏡の開発2021

    • Author(s)
      岩間 智紀,井上(安田) 久美, 末永 智一, 珠玖 仁
    • Organizer
      第6回東北大学若手研究者アンサンブルワークショップ
    • Related Report
      2020 Research-status Report
  • [Presentation] クローズドバイポーラ電極を用いるバイオセンシング・バイオイメージングデバイスの開発2020

    • Author(s)
      井上(安田) 久美, シティ マストゥラ, 岩間 智紀, 珠玖 仁, 末永 智一
    • Organizer
      第30回日本MRS年次大会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 導体ペースト塗り込み法を用いたバイポーラ電極アレイの作製と高時空間分解イメージングへの応用2020

    • Author(s)
      岩間 智紀,井上(安田) 久美,阿部 博弥,末永 智一,珠玖 仁
    • Organizer
      第30回 日本MRS年次大会
    • Related Report
      2020 Research-status Report
  • [Presentation] バイポーラ電極アレイを利用する電気化学イメージングの原理検証2020

    • Author(s)
      井上(安田)久美、岩間智紀、小松万葉、末永智一、珠玖仁
    • Organizer
      第66回ポーラログラフィーおよび電気分析化学討論会
    • Related Report
      2020 Research-status Report
  • [Presentation] Closed bipolar electrochemistry for sensor and bioimaging applications2020

    • Author(s)
      K. Y. Inoue, T. Iwama, Siti Masturah Fakhruddin, H. Shiku and T. Matsue
    • Organizer
      PRiMe 2020
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Demonstration of monoamine detection and 2D imaging of its distribution using cathodic electrochemiluminescence on closed bipolar electrodes2020

    • Author(s)
      化学系学協会東北大会
    • Organizer
      Mayo Komatsu, Tomoki Iwama, Kumi.Y Inoue, Kosuke Ino, Hitoshi Shiku
    • Related Report
      2020 Research-status Report
  • [Presentation] バイオセンサーを用いた日常人間ドック2020

    • Author(s)
      井上(安田) 久美
    • Organizer
      第20回日本抗加齢医学会総会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] バイポーラ電極および電気化学発光を用いた高時空間分解能顕微鏡システムの開発2020

    • Author(s)
      岩間 智紀 ・ 井上(安田) 久美1 ・ 阿部 博弥2 ・ 末永 智一 ・ 珠玖 仁
    • Organizer
      日本分析化学会 第69年会
    • Related Report
      2020 Research-status Report
  • [Presentation] Demonstration of the novel imaging system based on bipolar electrochemistry for high spatio-temporal resolution bioimaging2020

    • Author(s)
      Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      71st Annual Meeting of the International Society of Electrochemistry
    • Related Report
      2020 Research-status Report
  • [Presentation] バイポーラ電気化学顕微鏡システムの開発と高時空間分解能バイオイメージング応用の検討2020

    • Author(s)
      岩間智紀、井上(安田)久美、阿部博弥、末永智一、珠玖 仁
    • Organizer
      電気化学会第87回大会(名古屋工業大学)※新型コロナウイルス感染症対策のためWeb討論会に変更
    • Related Report
      2019 Research-status Report
  • [Presentation] 電気化学顕微鏡システムを用いた生体分子の高時空間分解能イメージングの検討2020

    • Author(s)
      岩間智紀、小松万葉、井上(安田)久美、阿部博弥、末永智一、珠玖仁
    • Organizer
      第9回東北脳科学ウィンタースクール (鳴子温泉郷中山平温泉 仙庄館)
    • Related Report
      2019 Research-status Report
  • [Presentation] Bipolar electrochemistry based novel imaging system for high spatio-temporal resolution bioimaging2019

    • Author(s)
      Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      CEMSupra 2019 (Ito Hall, The University of Tokyo, Japan)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Closedバイポーラ電極アレイを用いた高時空間分解能イメージングシステムによる生体活動観察2019

    • Author(s)
      岩間智紀、井上(安田)久美、阿部博弥、末永智一、珠玖 仁
    • Organizer
      第29回日本MRS年次大会 (横浜情報文化センター・横浜市開港記念館)
    • Related Report
      2019 Research-status Report
  • [Presentation] Application of a bipolar electrode junction in the development of a multiplexed liquid junction-free biosensing system2019

    • Author(s)
      S. M. Fakhruddin, K. Y. Inoue, T. Matsue, H. Shiku
    • Organizer
      第29回日本MRS年次大会 (横浜情報文化センター・横浜市開港記念館)
    • Related Report
      2019 Research-status Report
  • [Presentation] Chemical imaging system using closed bipolar electrode array2019

    • Author(s)
      Kumi. Y. Inoue, Tomoki Iwama, Hitoshi Shiku, Tomokazu Matsue
    • Organizer
      The 13th Asian Conference on Chemical Sensors (ACCS2019) (Grand Inna Sanur Bali, Indonesia)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] High Spatial-Temporal Electrochemical Imaging System using Closed Bipolar Electrode Array and observation of Biological phenomena.2019

    • Author(s)
      Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      The International Joint Meeting of the Polarographic Society of Japan (PSJ) and National Taiwan University (NTU) (National Taiwan University, Taipei, Taiwan)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analytical applications of closed bipolar electrode array and consideration of its operating principle.2019

    • Author(s)
      Kumi Y. Inoue, Siti Masturah Fakhruddin, Tomoki Iwama, Miho Ikegawa, Hitoshi Shiku, Tomokazu Matsue.
    • Organizer
      The International Joint Meeting of the Polarographic Society of Japan (PSJ) and National Taiwan University (NTU) (National Taiwan University, Taipei, Taiwan)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of a high spatio-temporal resolution electrochemical imaging system using a closed bipolar electrode array2019

    • Author(s)
      Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue and Hitoshi Shiku
    • Organizer
      The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2019) (Congress Center Basel, Basel, Switzerland)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] High spatio-temporal electrochemical imaging system using a closed bipolar electrode array and its biological application2019

    • Author(s)
      Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      RSC Tokyo International Conference 2019 (Makuhari Messe, Chiba, Japan)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] A Liquid Junction-free Electrochemical System by Substitution of Ionic Transport to Electron Transport through a Closed Bipolar Electrode2019

    • Author(s)
      S. M. Fakhruddin, K. Y. Inoue, T. Matsue, H. Shiku
    • Organizer
      RSC Tokyo International Conference 2019 (Makuhari Messe, Chiba, Japan)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Chemical imaging using a closed bipolar electrode array and its biological application2019

    • Author(s)
      Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      International Symposium on Analytical Electrochemistry 2019 (ISAE 2019) (Tohoku University, Sendai, Japan)
    • Related Report
      2019 Research-status Report
  • [Presentation] Development of a Liquid Junction-free Electrochemical System by Substitution of the Ionic Transport2019

    • Author(s)
      S. M. Fakhruddin, K. Y. Inoue, T. Matsue, H. Shiku
    • Organizer
      International Symposium on Analytical Electrochemistry 2019 (ISAE 2019) (Tohoku University, Sendai, Japan)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] High spatio-temporal resolution imaging using closed bipolar electrode array2019

    • Author(s)
      Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      The 3rd ECS Yamagata University Student Chapter Symposium (Yonezawa Campusu, Yamagata University)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Investigation of a Liquid Junction-free Electrochemical System by Substitution of Ionic Transport to Electron Transport through a Bipolar Electrode2019

    • Author(s)
      Siti Masturah Fakhruddin, Kumi Y. Inoue, Tomokazu Matsue. Hitoshi Shik
    • Organizer
      The 3rd ECS Yamagata University Student Chapter Symposium (Yonezawa Campusu, Yamagata University)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of a novel electrochemical imaging system for high spatio-temporal resolution imaging2019

    • Author(s)
      Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      The 11th International Symposium on Microchemistry and Microsystems (ISMM 2019) (Yangling International Convention Exhibition Hotel, Yangling, Shaanxi, China)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of a Liquid Junction-free Reference Electrode System by Application of Closed Bipolar Electrode (cBPE)2019

    • Author(s)
      Siti Masturah Fakhruddin, Kumi Y. Inoue, Tomokazu Matsue, Hitoshi Shiku
    • Organizer
      The 11th International Symposium on Microchemistry and Microsystems (ISMM 2019) (Yangling International Convention Exhibition Hotel, Yangling, Shaanxi, China)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Investigation of a Closed Bipolar Electrode Sensing Scheme with Liquid Junction-less Reference Electrode2019

    • Author(s)
      Siti Masturah Fakhruddin, K. Y. Inoue,R. Tsuga, M. Ikegawa, T. Matsue
    • Organizer
      日本化学会第99春季年会(甲南大学・岡山キャンパス)
    • Related Report
      2019 Research-status Report
  • [Book] 近未来のデジタルヘルスを支える酵素バイオ技術2022

    • Author(s)
      冨永昌人
    • Total Pages
      410
    • Publisher
      シーエムシー出版
    • ISBN
      9784781316604
    • Related Report
      2021 Annual Research Report
  • [Remarks] 分子の動きを捉える顕微鏡素子を熱延伸法で開発 ―量産化による生体内分子イメージングの実用化に期待―

    • URL

      https://www.tohoku.ac.jp/japanese/2021/11/press20211108-02-imaging.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 研究室ウェブページ/Publications

    • URL

      https://www.ccn.yamanashi.ac.jp/~inokumi/paper/index.html

    • Related Report
      2020 Research-status Report
  • [Remarks] 井上(安田)久美researchmap

    • URL

      https://researchmap.jp/read0155030

    • Related Report
      2019 Research-status Report
  • [Remarks] 旧末永研究室HP

    • URL

      http://www.che.tohoku.ac.jp/~bioinfo/

    • Related Report
      2019 Research-status Report
  • [Remarks] 珠玖研究室HP

    • URL

      https://www.che.tohoku.ac.jp/~est/index.html

    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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