Development of "RIGR (Recombinse-mediated In vivo Genomic Recording) system"
Project/Area Number |
19K05762
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38020:Applied microbiology-related
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Research Institution | National Institute of Infectious Diseases (2022) University of Tsukuba (2019-2021) |
Principal Investigator |
Abe Kimihiro 国立感染症研究所, 治療薬・ワクチン開発研究センター, 主任研究官 (10748940)
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Project Status |
Completed (Fiscal Year 2022)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 枯草菌 / バイオフィルム / セリンリコンビナーゼ / DNA組換え / 細胞分化 / serine recombinase / 部位特異的DNA組換え / 胞子形成 / recombinase / autoysin / membrane vesicle / 微生物集団 / 水平伝達 / 膜小胞 / 不均一性 |
Outline of Research at the Start |
本研究は、枯草菌をモデルとしたbiofilm形成メカニズムの解明を目指し、集団内の菌体ごとの役割分担 (細胞分化)を時系列的に解析する。これを達成するために、bacteriophage由来のserine recombinaseによる部位特異的DNA組換え反応を利用し、個々の菌体における細胞分化の変遷を情報としてゲノムDNA上に記録する技術 ”in vivoゲノム記録法(RIGR法; Recombinase-mediated In vivo Genomic Recording system)”を確立する。
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Outline of Final Research Achievements |
Many bacteria form communities called biofilms to survive harsh natural environments. It is known that individual bacterial cells play different roles in biofilms. Our research aimed to develop a novel methodology for analysis of bacterial behaviors in a biofilm, designated as "RIGR (Recombinse-mediated In vivo Genomic Recording) system", using Bacillus subtilis as the model organism. In this study, we established B. subitlis strains that can store a memory of cellular differentiation into sporulating or natural (DNA) competent cells in their genomic DNA. The RIGR system can be expected to contribute to a better understanding of bacterial biofilms in the future.
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Academic Significance and Societal Importance of the Research Achievements |
本研究の目的は、バイオフィルム中の個々の細菌の分化状態データを各細菌ゲノムDNAを記録媒体として保存する”in vivo ゲノム記録法(RIGR法)"の技術的基盤を確立することである。本手法を用いることで、自然環境中の細菌の暮らしぶりをよりリアルに知ることができ、ヒトと微生物のより良い共生関係の構築に寄与すると考えられる。
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Report
(5 results)
Research Products
(21 results)
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[Journal Article] Expansion of the spore surface polysaccharide layer in Bacillus subtilis by deletion of genes encoding glycosyltransferases and glucose modification enzymes2019
Author(s)
Bentley Shuster, Mark Khemmani, Yusei Nakaya, Gudrun Holland, Keito Iwamoto, Kimihiro Abe, Daisuke Imamura, Nina Maryn, Adam Driks, Tsutomu Sato, Patrick Eichenberger
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Journal Title
Journal of Bacteriology
Volume: 201(19)
Issue: 19
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Contributions of crust proteins to spore surface properties in Bacillus subtilis2019
Author(s)
Bentley Shuster, Mark Khemmani, Kimihiro Abe, Xiaoyu Huang, Yusei Nakaya, Nina Maryn, Sally Buttar, Adriana Gonzalez, Adam Driks, Tsutomu Sato, Patrick Eichenberger
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Journal Title
Molecular Microbiology
Volume: 111(3)
Issue: 3
Pages: 825-843
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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