Development of bio-degradative composite resin containing alpha-1,3-glucan and enzyme for its recycling

• Project/Area Number: 19K05803
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38020:Applied microbiology-related
• Research Institution: Ritsumeikan University
• Principal Investigator: WAKAYAMA MAMORU 立命館大学, 生命科学部, 教授 (70240455)
• Co-Investigator(Kenkyū-buntansha): 日び 隆雄 福井県立大学, 生物資源学部, 教授 (00285181) ; 武田 陽一 立命館大学, 生命科学部, 教授 (20423973)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2021: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: alpha-1,3-グルカン / alpha-1,3-glucanase / 生分解性樹脂 / X-線結晶構造解析 / 機能性 / カルボキシメチル化 / α-1,3-グルカン / α-1,3-グルカナーゼ / 再資源化

Outline of Research at the Start

α-1,3-グルカンのポリマーとしての利用法の開発やその分解酵素であるα-1,3-グルカナーゼの構造と機能ならびにその利用法に関する研究は大変遅れている。本研究では、α-1,3-グルカンを他の生分解性ポリマーと組合わせることで新規の機能を有する生分解性複合樹脂の開発を行うとともに、開発した樹脂の再資源化を環境にやさしく効率よくおこなうための酵素α-1,3-グルカナーゼの高機能化をセットで行うことを目的とする。

Outline of Final Research Achievements

Alpha-1,3-glucan, alpha-1,3-linked homopolymer of glucose, is the main component of extracellular polysaccharides synthesized from sucrose from Streptococcus mutans, bacteria found in tooth cavities and the cell wall component of some filamentous fungi. There have been little reports on synthesis and degradation of this glucan. Application of alpha-1,3-glucan has recently been received increased attention. We have developed enzymatic synthesis of alpha-1,3-glucan by using glycosyltransferase I from oral Streptococcus mutans and clarified three-dimensional structure of alpha-1,3-glucanases which are responsible for degradation of alpha-1,3-glucan first in the world. In this study, we prepared partially carboxymethylated alpha-1,3-glucan with biodegradative property and performed characteristic analysis.

Academic Significance and Societal Importance of the Research Achievements

持続可能な社会の構築を目指して様々な取り組みが行われている。我々の周りは石油を原料とする難分解性プラスティックで満ち溢れ、それが生態系の維持を脅かしていることから、これらの石油由来のプラスティックを使用しない取り組みが進みつつある。一方で生分解性プラスティックの需要は高まっており、本研究対象であるalpha-1,3-グルカンも微生物酵素によって分解可能なポリマーであり、そのカルボキシメチル誘導体も酵素分解、微生物分解が可能な環境にやさしい素材としての利用が期待できる。特に、その分解に関わる酵素の研究について、その立体構造を世界に先駆けて解明したことは本研究成果の重要な成果である。

Research Products

• [Journal Article] Functional analysis of α-1,3-glucanase domain structure from <i>Streptomyces thermodiastaticus</i> HF3-3 (2021)
  • Author(s): Panti N, Cherdvorapong V, Itoh T, Hibi T, Suyotha W, Yano S, Wakayama M
  • Journal Title: The Journal of General and Applied Microbiology Volume: 67 Issue: 3 Pages: 85-91
  • DOI: 10.2323/jgam.2020.07.003
  • NAID: 130008069727
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed / Open Access
• [Journal Article] Prevention of oral biofilm formation and degradation of biofilm by recombinant α-1,3-glucanases from <i>Streptomyces thermodiastaticus</i> HF3-3 (2020)
  • Author(s): Cherdvorapong V, Panti N, Suyotha W, Tsuchiya Y, Toyotake Y, Yano S, Wakayama M
  • Journal Title: The Journal of General and Applied Microbiology Volume: 66 Issue: 5 Pages: 256-264
  • DOI: 10.2323/jgam.2019.11.003
  • NAID: 130007947208
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Crystal structure of the catalytic unit of thermostable GH87 α-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3 (2020)
  • Author(s): Itoh Takafumi、Panti Niphawan、Hayashi Junji、Toyotake Yosuke、Matsui Daisuke、Yano Shigekazu、Wakayama Mamoru、Hibi Takao
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 533 Issue: 4 Pages: 1170-1176
  • DOI: 10.1016/j.bbrc.2020.09.133
  • Peer Reviewed / Open Access
• [Journal Article] Crystal structure of the catalytic unit of GH87-type α-1,3-glucanase Agl-KA from Bacillus circulans (2019)
  • Author(s): S. Yano, W. Suyotha, N. Oruro, T. Matsui, S. Shiga, T. Itoh, T. Hibi, Y. Tanaka, M. Wakayama, K. Makabe
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 15259-15259
  • DOI: 10.1038/s41598-019-51822-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural insights into substrate recognition and catalysis by glycoside hydrolase family 87 α-1,3-glucanase from Paenibacillus glycanilnticus FH11 (2019)
  • Author(s): T. Itoh, R. Intuy, W. Suyotha, J. Hayashi, S. Yano, K. Makable, M. Wakayama, T. Hibi
  • Journal Title: FEBS Journal Volume: - Issue: 12 Pages: 1-20
  • DOI: 10.1111/febs.15161
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] α-1,3-グルカンを用いた新規機能性素材の開発 (2021)
  • Author(s): 小倉太一, 大垣内誠, 豊竹洋佑, 松井大亮, 若山守
  • Organizer: 第73回日本生物工学会大会
• [Presentation] Domain structure and function of α-1,3-Glucanase from Streptomyces thermodiastaticus HF3-3 (2019)
  • Author(s): Niphawan Panti, Vipavee Cherdvorapong, Wassana Suyotha, Kazuyoshi Takagi, Shigekazu Yano, Yosuke Toyotake and Mamoru Wakayama
  • Organizer: 第71回日本生物工学会大会
• [Presentation] Characterization of catalytic α-1,3-glucanase isozymes from Paenibacillus glycanilyticus FH11 by using Brevibacillus system; Essential for suppression and degradation of Streptococcus mutans biofilms (2019)
  • Author(s): Intuy Rattanaporn, Hibi Takao, Itoh Takafumi, Suyotha Wasana, Yano, Shigekazu, Mamoru Wakayama
  • Organizer: 第71回日本生物工学会大会
• [Presentation] Inhibition of Streptococcus biofilm formation and its degradation by Streptomyces α-1,3-glucanases (2019)
  • Author(s): Mamoru Wakayama, Vipavee Cherdvorapong, Yosuke Toyotake
  • Organizer: 6th World Congress and Expo on Applied Microbiology
  • Int'l Joint Research
• [Presentation] Structure and Function of α-1,3-Glucanase from Streptomyces thermodiastaticus HF3-3 (2019)
  • Author(s): Niphawan Panti, Vipavee Cherdvorapong, Wassana Suyotha, Kazuyoshi Takagi, Shigekazu Yano, Yosuke Toyotake and Mamoru Wakayama
  • Organizer: International Conference on Enzymology and Molecular Biology, Enzyme2019
  • Int'l Joint Research
• [Presentation] Characterization of catalytic α-1,3-glucanase isozymes from Paenibacillus glycanilyticus FH11 by using Brevibacillus system; Essential for suppression and degradation of Streptococcus mutans biofilms (2019)
  • Author(s): Intuy Rattanaporn, Hibi Takao, Itoh Takafumi, Suyotha Wasana, Yano, Shigekazu, Mamoru Wakayama
  • Organizer: Biochemical and Molecular Engineering XXI
  • Int'l Joint Research