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Functional analysis of strigolactone biosynthetic enzymes for developing a control method of root parasitic weeds

Research Project

Project/Area Number 19K05838
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38040:Bioorganic chemistry-related
Research InstitutionUtsunomiya University

Principal Investigator

Nomura Takahito  宇都宮大学, バイオサイエンス教育研究センター, 准教授 (60373346)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsストリゴラクトン / 根寄生雑草 / 生合成酵素 / 生合成 / 酵素 / ストライガ / 根寄生植物
Outline of Research at the Start

根寄生雑草(特にストライガ類)はアフリカ諸国の農作物生産に甚大な被害を与えている。また、地中海沿岸諸国における根寄生雑草(特にオロバンキ類)の被害も顕在化している。現在のところ日本には農作物に大きな被害を与えている根寄生雑草種は侵入していないと考えられるが潜在的な脅威となっている。根寄生雑草の種子は宿主植物の根から分泌されているストリゴラクトンと呼ばれる化合物を認識して発芽する。本研究は、根寄生雑草の防除法の開発基盤となりうるストリゴラクトンの生合成酵素の機能の解明を行う。

Outline of Final Research Achievements

Root parasitic plants parasitize on the roots of host plants and deprive nutrients and water, causing extensive damage to crops around the world. So far, no effective control method for root parasitic weeds has been established. Root parasitic weed seeds germinate by recognizing secondary metabolites called strigolactones (SLs), which are exudated from the roots of host plants. The lgs1 mutant of sorghum that exhibits resistance to root parasitic weeds has been reported. The LGS1 gene was thought to encode an SL biosynthetic enzyme, but its function has not been elucidated. This study has revealed the SL biosynthetic pathway catalyzed by cytochrome P450 and sulfotransferase in sorghum.

Academic Significance and Societal Importance of the Research Achievements

根寄生雑草(特にストライガ類)はアフリカ諸国の農作物の収量を大きく低下させている。また、地中海沿岸諸国やオーストラリアといった農業先進国における根寄生雑草(特にオロバンキ類)の被害も顕在化している。現在のところ日本には農作物に大きな被害を与えている根寄生雑草種は侵入していないと考えられるが潜在的な脅威となっている。有効な防除法のない根寄生雑草に関する研究を行う意義は大きく、本研究の成果は根寄生雑草を制御する技術の基盤となりうる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (26 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (10 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (11 results) (of which Int'l Joint Research: 2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] カリフォルニア大学リバーサイド校(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] カルフォルニア大学リバーサイド校(米国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] カルフォルニア大学リバーサイド校(米国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Origins and evolution of the dual functions of strigolactones as rhizosphere signaling molecules and plant hormones2022

    • Author(s)
      Kyozuka Junko、Nomura Takahito、Shimamura Masaki
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 65 Pages: 102154-102154

    • DOI

      10.1016/j.pbi.2021.102154

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum2021

    • Author(s)
      Yoda Akiyoshi、Mori Narumi、Akiyama Kohki、Kikuchi Mayu、Xie Xiaonan、Miura Kenji、Yoneyama Kaori、Sato‐Izawa Kanna、Yamaguchi Shinjiro、Yoneyama Koichi、Nelson David C.、Nomura Takahito
    • Journal Title

      New Phytologist

      Volume: 232 Issue: 5 Pages: 1999-2010

    • DOI

      10.1111/nph.17737

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation and Quantification of Natural Strigolactones from Root Exudates2021

    • Author(s)
      Xie Xiaonan、Yoneyama Kaori、Nomura Takahito、Yoneyama Koichi
    • Journal Title

      Strigolactones. Methods in Molecular Biology

      Volume: 2309 Pages: 3-12

    • DOI

      10.1007/978-1-0716-1429-7_1

    • ISBN
      9781071614280, 9781071614297
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An ancestral role of strigolactones as a rhizosphere signaling molecule2021

    • Author(s)
      経塚 淳子、野村 崇人
    • Journal Title

      Regulation of Plant Growth & Development

      Volume: 56 Issue: 2 Pages: 63-70

    • DOI

      10.18978/jscrp.56.2_63

    • ISSN
      1346-5406, 2189-6305
    • Related Report
      2021 Annual Research Report
  • [Journal Article] Triflumizole as a Novel Lead Compound for Strigolactone Biosynthesis Inhibitor.2020

    • Author(s)
      Kawada K., Uchida Y., Takahashi I., Nomura T., Sasaki Y., Asami T., Yajima S., Ito S.
    • Journal Title

      Molecules

      Volume: 25 Issue: 23 Pages: 5525-5525

    • DOI

      10.3390/molecules25235525

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis2020

    • Author(s)
      Yoneyama Kaori、Akiyama Kohki、Brewer Philip B.、Mori Narumi、Kawano‐Kawada Miyuki、Haruta Shinsuke、Nishiwaki Hisashi、Yamauchi Satoshi、Xie Xiaonan、Umehara Mikihisa、Beveridge Christine A.、Yoneyama Koichi、Nomura Takahito
    • Journal Title

      Plant Direct

      Volume: 4 Issue: 5 Pages: 1-14

    • DOI

      10.1002/pld3.219

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Do phosphate and cytokinin interact to regulate strigolactone biosynthesis or act independently?2020

    • Author(s)
      Kaori Yoneyama et al
    • Journal Title

      frontiers in Plant Science

      Volume: in press Pages: 1-9

    • DOI

      10.3389/fpls.2020.00438

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chemical identification of 18-hydroxycarlactonoic acid as an LjMAX1 product and in planta conversion of its methyl ester to canonical and non-canonical strigolactones in Lotus japonicus2020

    • Author(s)
      Mori Narumi、Sado Aika、Xie Xiaonan、Yoneyama Kaori、Asami Kei、Seto Yoshiya、Nomura Takahito、Yamaguchi Shinjiro、Yoneyama Koichi、Akiyama Kohki
    • Journal Title

      Phytochemistry

      Volume: 174 Pages: 112349-112365

    • DOI

      10.1016/j.phytochem.2020.112349

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of two oxygenase genes involved in the respective biosynthetic pathways of canonical and non-canonical strigolactones in Lotus japonicus.2020

    • Author(s)
      Mori N., Nomura T., Akiyama K.
    • Journal Title

      Planta

      Volume: 251 Issue: 2 Pages: 40-40

    • DOI

      10.1007/s00425-019-03332-x

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genome sequence of Striga asiatica provides insight into the evolution of plant parasitism.2019

    • Author(s)
      Yoshida S. et al.
    • Journal Title

      Current Biology

      Volume: 29 Issue: 18 Pages: 3041-3052

    • DOI

      10.1016/j.cub.2019.07.086

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 新規ストリゴラクトンbryosymbiolの生合成2021

    • Author(s)
      依田彬義、謝 肖男、児玉恭一、島崎翔太、秋山康紀、米山香織、嶋村正樹、経塚淳子、野村崇人
    • Organizer
      植物化学調節学会第56回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 新奇根寄生性植物防除法の確立を目的としたストリゴラクトン生合成阻害剤の創製2021

    • Author(s)
      川田 紘次郎、高橋 郁夫、野村 崇人、佐々木 康幸、浅見 忠男、矢嶋 俊介、伊藤 晋作
    • Organizer
      植物化学調節学会第56回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Strigolactone biosynthesis catalyzed by LGS1 in sorghum2021

    • Author(s)
      Akiyoshi Yoda、Narumi Mori、Kohki Akiyama、Xiaonan Xie、Kaori Yoneyama、Koichi Yoneyama、David C. Nelson、Takahito Nomura
    • Organizer
      3rd International Congress on Strigolactone
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 陸上植物に保存された祖先型ストリゴラクトン2021

    • Author(s)
      依田彬義,謝肖男,島崎翔太,児玉恭一,米山香織,秋山康紀,嶋村正樹,経塚淳子,野村崇人
    • Organizer
      第62回日本植物生理学会
    • Related Report
      2020 Research-status Report
  • [Presentation] ストリゴラクトン生産を阻害する新規化合物の創製2021

    • Author(s)
      川田紘次郎,高橋郁夫,小野田聡志,野村崇人, 佐々木康幸,浅見忠男,矢嶋俊介,伊藤晋作
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] コケ植物におけるストリゴラクトン生合成2021

    • Author(s)
      依田彬義,謝肖男,島崎翔太,児玉恭一,米山香織, 秋山康紀,嶋村正樹,経塚淳子,野村崇人
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 根寄生雑草防除の標的酵素ソルガム LGS1 の機能解析2020

    • Author(s)
      野村崇人、依田彬義、森 愛美、三浦謙治、米山香織、謝 肖男、秋山康紀、米山弘一
    • Organizer
      日本農薬学会第45回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 硫酸基転移酵素 LGS1 に触媒されるストリゴラクトン生合成経路2020

    • Author(s)
      依田彬義、森 愛美、謝 肖男、米山香織、三浦謙治、秋山康紀、米山弘一、野村崇人
    • Organizer
      第61回日本植物生理学会
    • Related Report
      2019 Research-status Report
  • [Presentation] シダ植物のストリゴラクトン2019

    • Author(s)
      藤倉 優、依田彬義、謝 肖男、米山香織、秋山康紀、経塚淳子、米山弘一、野村崇人
    • Organizer
      植物化学調節学会第54回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ソルガムにおける5-deoxystrigol生合成経路の解析2019

    • Author(s)
      依田彬義、森 愛美、謝 肖男、米山香織、三浦謙治、山口信次郎、秋山康紀、米山弘一、野村崇人
    • Organizer
      植物化学調節学会第54回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Biochemical characterization of sorghum LGS1 enzyme involved in strigolactone biosynthesis2019

    • Author(s)
      Akiyoshi Yoda, Narumi Mori, Xiaonan Xie, Kaori Yoneyama, David Nelson, Kohki Akiyama, Koichi Yoneyama, Takahito Nomura
    • Organizer
      The 23rd International Conference on Plant Growth Substances
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Remarks] [プレスリリース]根寄生雑草の寄生を制御する酵素の解明

    • URL

      https://www.utsunomiya-u.ac.jp/topics/research/009293.php

    • Related Report
      2021 Annual Research Report
  • [Patent(Industrial Property Rights)] タンパク質の発現方法およびタンパク質発現用ベクター2019

    • Inventor(s)
      野村崇人、彬義依田、鈴木智大
    • Industrial Property Rights Holder
      野村崇人、彬義依田、鈴木智大
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-130070
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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