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Studies on hyper-sensitive strigolactone perception mechanism by parasitic plants

Research Project

Project/Area Number 19K05852
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38040:Bioorganic chemistry-related
Research InstitutionMeiji University

Principal Investigator

Seto Yoshiya  明治大学, 農学部, 専任准教授 (40620282)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsストリゴラクトン / 根寄生植物 / Striga asiatica / Orobanche minor / 受容体 / Striga hermonthica / 加水分解酵素 / HTL
Outline of Research at the Start

根寄生植物はアフリカなどの農地において甚大な被害をもたらしている。根寄生植物は、食いつく相手となる植物の根から放出されるストリゴラクトン(SL)と名付けられた化合物によって宿主の存在を認識して発芽する。その際、SLに対して、極めて高い感受性を獲得することにより、宿主となる植物を効率的に見出すことを可能としている。本研究では、根寄生植物におけるSL受容体が如何にして超高感度にSLを認識しているのか、その分子メカニズムの解明に挑戦する。

Outline of Final Research Achievements

The germination of root parasitic plants is induced by the host-derived chemical signals, strigolactone (SL). In a root parasitic plant, Striga hermonthica, more than 10 SL receptors have been identified, one of which exhibited extremely high sensitivity toward SL. However, the molecular mechanism for the sensitive SL recognition mechanism has not been clarified. Toward the identification of the hyper sensitive SL perception mechanism, we identified the SL receptors in Striga asiatica, and Orobanche minor. We successfully identified highly sensitive receptors from each plant species. We also found a candidate amino acid in the highly sensitive receptor sequence. We are currently analyzing the importance of this amino acid by a site directed mutation approach.

Academic Significance and Societal Importance of the Research Achievements

本研究では、これまでSL受容体が同定されていなかった2種の根寄生植物から、SL受容体を同定することに成功した。いずれの種においても、SLに対する感受性が高い受容体を1つずつ同定することができており、今後、これらのアミノ酸配列を感受性が高くない受容体のアミノ酸配列と比較することにより、感受性を制御するメカニズムの理解につながることが期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (9 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (3 results) (of which Invited: 1 results)

  • [Int'l Joint Research] The Salk Institute(米国)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] A New Series of Strigolactone Analogs Derived From Cinnamic Acids as Germination Inducers for Root Parasitic Plants2022

    • Author(s)
      Suzuki Taiki、Kuruma Michio、Seto Yoshiya
    • Journal Title

      Frontiers in Plant Science

      Volume: 13

    • DOI

      10.3389/fpls.2022.843362

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tryptophan derivatives regulate the seed germination and radicle growth of a root parasitic plant, Orobanche minor2021

    • Author(s)
      Kuruma Michio、Suzuki Taiki、Seto Yoshiya
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 43 Pages: 128085-128085

    • DOI

      10.1016/j.bmcl.2021.128085

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ligand-receptor interactions in plant hormone signaling2021

    • Author(s)
      Takeuchi Jun、Fukui Kosuke、Seto Yoshiya、Takaoka Yousuke、Okamoto Masanori
    • Journal Title

      The Plant Journal

      Volume: 105 Issue: 2 Pages: 290-306

    • DOI

      10.1111/tpj.15115

    • NAID

      120006941098

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Strigolactone biosynthesis, transport and perception2020

    • Author(s)
      Mashiguchi Kiyoshi、Seto Yoshiya、Yamaguchi Shinjiro
    • Journal Title

      The Plant Journal

      Volume: 105 Issue: 2 Pages: 335-350

    • DOI

      10.1111/tpj.15059

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome sequence of Striga asiatica provides insight into the evolution of plant parasitism.2019

    • Author(s)
      Yoshida S. et al.
    • Journal Title

      Current Biology

      Volume: 29 Issue: 18 Pages: 3041-3052

    • DOI

      10.1016/j.cub.2019.07.086

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ストリゴラクトン様活性分子を生産する微生物の探索2021

    • Author(s)
      瀬戸義哉
    • Organizer
      2021年度日本農芸化学会北海道支部第1回学術講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ケイヒ酸を骨格とした新規ストリゴラクトンアナログの合成2021

    • Author(s)
      鈴木泰輝、来馬道生、大杉、佳乃、瀬戸義哉
    • Organizer
      植物化学調節学会第56回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 根寄生植物の発芽に及ぼすトリプトファン関連分子の作用について2021

    • Author(s)
      来馬道生、鈴木泰輝、瀬戸義哉
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2021 Annual Research Report

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Published: 2019-04-18   Modified: 2023-01-30  

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