Verification of germline development ensured by centromere DNA methylation.
| Project/Area Number |
19K05956
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38060:Applied molecular and cellular biology-related
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| Research Institution | Kitasato University |
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Principal Investigator |
Yamazaki Taiga 北里大学, 北里大学メディカルセンター, 上級研究員 (90524231)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNAメチル化 / エピゲノム編集 / セントロメア / 生殖細胞 / 初期胚発生 / ペリセントロメア |
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| Outline of Research at the Start |
本研究は生殖細胞に特徴的なセントロメアDNA低メチル化の生物学的機能を明らかにすることを目的としている。エピゲノム編集によってセントロメア特異的にDNAメチル化を亢進させた生殖細胞を作製し、この生殖細胞の発生動態を追跡することで、生殖細胞形成におけるセントロメアDNAメチル化機能について解析を行う。
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| Outline of Final Research Achievements |
In this study, we established an epigenome editing technology that can modify DNA methylation of specific genomic sequences and applied it to mouse embryos to analyze DNA methylation that plays an important role in early embryonic development. We found that fertilized embryos which normally show hypomethylated centromeres, exhibited significant developmental defects when hypomethylated centromeres were artificially methylated by epigenome-editing. This result indicates that centromere DNA hypomethylation functions as an important epigenome in early mouse embryogenesis.
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| Academic Significance and Societal Importance of the Research Achievements |
本解析手法は特定ゲノム配列のエピゲノムと、それに関与する生物学的事象との因果関係を明らかにする事が可能であり、本研究によって実際の実施例が示された事は特筆すべき成果である。また、本手法は学術的な知見を得るための手法のみならず、遺伝子発現制御を介した疾患治療などの応用研究につながる可能性を秘めている事から今後の発展が期待される。
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Report
(5 results)
Research Products
(11 results)
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[Journal Article] Effects of Roxadustat on Erythropoietin Production in the Rat Body2022
Author(s)
Yasuoka Yukiko,Izumi Yuichiro,Fukuyama Takashi,Omiya Haruki,Pham Truyen D.,Inoue Hideki,Oshima Tomomi,Yamazaki Taiga,Uematsu Takayuki,Kobayashi Noritada,Shimada Yoshitaka,Nagaba Yasushi,Yamashita Tetsuro,Mukoyama Masashi,Sato Yuichi,Wall Susan M.,Sands Jeff M.,Takahashi Noriko,Kawahara Katsumasa,Nonoguchi Hiroshi
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Journal Title
Molecules
Volume: 27
Issue: 3
Pages: 1119-1119
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation2020
Author(s)
Yukiko Yasuoka, Takashi Fukuyama, Yuichiro Izumi, Yushi Nakayama, Hideki Inoue, Kengo Yanagita, Tomomi Oshima, Taiga Yamazaki, Takayuki Uematsu 2, Noritada Kobayashi 2, Yoshitaka Shimada 5, Yasushi Nagaba, Masashi Mukoyama, Tetsuro Yamashita, Yuichi Sato, Jeff M Sands, Katsumasa Kawahara, Hiroshi Nonoguchi
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Journal Title
Physiol Rep.
Volume: 12
Issue: 12
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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