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Understanding of molecular mechanisms to destine to form a constitutive barrier to radial oxygen loss in wild rice species

Research Project

Project/Area Number 19K05978
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39010:Science in plant genetics and breeding-related
Research InstitutionFukui Prefectural University

Principal Investigator

Shiono Katsuhiro  福井県立大学, 生物資源学部, 准教授 (20610695)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords湿害 / 洪水 / 低酸素 / 野生イネ / 気候変動
Outline of Research at the Start

酸素漏出バリアはイネなどの水が多い環境に適応した湿生植物がもつ耐湿性の重要形質である。イネは過湿ストレスを受けてから誘導的にバリアを形成するが、これまでのイネをモデルとした形成機構の研究ではバリア形成を決定づける上流の鍵遺伝子は特定できていない。最近、申請者はアマゾン川流域に分布するAAゲノムの野生イネの中に過湿ストレスを受ける前から恒常的に酸素漏出バリアを形成するエコタイプを見つけた。本研究ではこのバリアの誘導性の異なる野生イネと栽培イネを用いて遺伝子発現の比較解析を行う。これにより、酸素漏出バリア形成の誘導性を決定づける上流の制御遺伝子群の特定を目指す。

Outline of Final Research Achievements

A barrier against radial oxygen loss (ROL) is a crucial trait for waterlogging tolerance in wetland plants. Rice inducibly forms the ROL barrier under waterlogged conditions. Therefore, rice has been used as a model to understand the molecular mechanisms of the barrier formation mechanism. But, it has been not identified upstream key genes that determine barrier formation. This study identified an ecotype in the AA-genome wild-rice O. glumaepatula, distributed in the Amazon River basin, forming an ROL barrier before suffering waterlogging stress. We found several lines forming a constitutive ROL barrier in the introgression lines of O. glumaepatula in the genetic background of O. sativa (cv. Taichung 65). Research has progressed toward identifying the gene(s) responsible for forming the constitutive barrier in wild rice.

Academic Significance and Societal Importance of the Research Achievements

酸素漏出バリアは湿生植物がもつ耐湿性の重要形質である。本研究で、過湿ストレスを受ける前から恒常的に酸素漏出バリアを形成する野生イネのエコタイプを特定できた。さらに、この野生イネの染色体断片を栽培イネに導入すると、野生イネ同様に、恒常的にバリアを形成させることができた。既存のイネをモデルとした形成機構の研究では到達できない、バリア形成を決定づける、上流に位置する鍵遺伝子の特定に向けて、研究が進展した。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (23 results)

All 2021 2020 2019 Other

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 4 results) Remarks (3 results)

  • [Journal Article] Measurement of plant tissue porosity: II. Archimedes method2021

    • Author(s)
      MIYASHITA Tomoki、EJIRI Masato、SHIMAMURA Satoshi、YAMAUCHI Takaki、SHIONO Katsuhiro
    • Journal Title

      Root Research

      Volume: 30 Issue: 2 Pages: 41-45

    • DOI

      10.3117/rootres.30.41

    • NAID

      130008058479

    • ISSN
      0919-2182, 1880-7186
    • Year and Date
      2021-06-20
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Abscisic acid is required for exodermal suberization to form a barrier to radial oxygen loss in the adventitious roots of rice ( <i>Oryza sativa</i> )2021

    • Author(s)
      Shiono Katsuhiro、Yoshikawa Marina、Kreszies Tino、Yamada Sumiyo、Hojo Yuko、Matsuura Takakazu、Mori Izumi C.、Schreiber Lukas、Yoshioka Toshihito
    • Journal Title

      New Phytologist

      Volume: 233 Issue: 2 Pages: 655-669

    • DOI

      10.1111/nph.17751

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A barrier to radial oxygen loss helps the root system cope with waterlogging-induced hypoxia2021

    • Author(s)
      Ejiri Masato、Fukao Takeshi、Miyashita Tomoki、Shiono Katsuhiro
    • Journal Title

      Breeding Science

      Volume: 71 Issue: 1 Pages: 40-50

    • DOI

      10.1270/jsbbs.20110

    • NAID

      130007991315

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Groups of multi-cellular passage cells in the root exodermis of Echinochloa crus-galli varieties lack not only suberin lamellae but also lignin deposits2020

    • Author(s)
      Ejiri Masato、Shiono Katsuhiro
    • Journal Title

      Plant Signaling & Behavior

      Volume: 15 Issue: 2 Pages: 1719749-1719749

    • DOI

      10.1080/15592324.2020.1719749

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 質量分析イメージングによる複数の植物ホルモンの可視化2020

    • Author(s)
      塩野克宏、平修
    • Journal Title

      植物の生長調節

      Volume: 55 Pages: 84-91

    • NAID

      130008131343

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Some Accessions of Amazonian Wild Rice (Oryza glumaepatula) Constitutively Form a Barrier to Radial Oxygen Loss along Adventitious Roots under Aerated Conditions2020

    • Author(s)
      Ejiri Masato、Sawazaki Yuto、Shiono Katsuhiro
    • Journal Title

      Plants

      Volume: 9 Issue: 7 Pages: 880-880

    • DOI

      10.3390/plants9070880

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Imaging of Multiple Plant Hormones in Roots of Rice (Oryza sativa) Using Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry2020

    • Author(s)
      Katsuhiro Shiono 、Shu Taira
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 0 Issue: 24 Pages: 6770-6775

    • DOI

      10.1021/acs.jafc.0c00749

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] アマゾン川流域に分布する野生イネ(Oryza glumaepatula)がもっている洪水耐性を利用した育種の可能性2020

    • Author(s)
      江尻真斗, 宮下智貴, 塩野克宏
    • Journal Title

      アグリバイオ

      Volume: 4 Pages: 80-82

    • NAID

      40022337835

    • Related Report
      2020 Research-status Report
  • [Presentation] AAゲノムを持つ野生イネOryza glumaepatulaの種子根における形態と解剖学的特徴2021

    • Author(s)
      江尻真斗, 塩野克宏
    • Organizer
      第53回根研究集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 根箱・ピンボード法を用いた野生イネOryza glumaepatulaの根系評価2021

    • Author(s)
      宮下智貴, 塩野克宏
    • Organizer
      第53回根研究集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 非接触スキャナを用いた地表根評価手法の効率化2021

    • Author(s)
      宮下智貴, 塩野克宏
    • Organizer
      第54回根研究集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イネの酸素漏出バリア誘導のトリガーを感知する組織の探索2021

    • Author(s)
      江岸祐夏, 塩野克宏
    • Organizer
      第54回根研究集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Oryza glumaepatula IRGC105668 系統の恒常的酸素漏出バリア形成能力の評価2021

    • Author(s)
      江尻真斗,塩野克宏
    • Organizer
      第139回日本育種学会
    • Related Report
      2020 Research-status Report
  • [Presentation] 根箱・ピンボード法を用いた湛水処理により誘導されるOryza glumaepatulaの地表根の評価2020

    • Author(s)
      宮下智貴, 塩野克宏
    • Organizer
      第52回根研究集会
    • Related Report
      2020 Research-status Report
  • [Presentation] Evaluation of barrier to radial oxygen loss in wild rice species, Oryza rufipogon and O. glumaepatula.2019

    • Author(s)
      Masato Ejiri, Katsuhiro Shiono
    • Organizer
      13th International Society of Plant Anaerobiosis Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Cytokinin is involved in a formation of barrier to radial oxygen loss along the adventitious roots in rice (Oryza sativa)2019

    • Author(s)
      Kana Shimizu, Katsuhiro Shiono
    • Organizer
      13th International Society of Plant Anaerobiosis Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Abscisic acid is required for root suberization at the exodermis to form a barrier to radial oxygen loss in rice (Oryza sativa)2019

    • Author(s)
      Katsuhiro Shiono, Marina Yoshikawa, Tino Kreszies, Sumiyo Yamada, Yuko Hojo, Takakazu Matsuura, Izumi C. Mori, Lukas Schreiber, Toshihito Yoshioka
    • Organizer
      13th International Society of Plant Anaerobiosis Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of unsuberized passage-cells at the exodermis in annual wild species of Echinochloa2019

    • Author(s)
      Katsuhiro Shiono, Masato Ejiri
    • Organizer
      4th Plant Apoplastic Diffusion Barriers 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 耐湿性の重要形質である酸素漏出バリアを恒常的に形成する野生イネの探索2019

    • Author(s)
      江尻真斗, 塩野克宏
    • Organizer
      第136回講演会日本育種学会
    • Related Report
      2019 Research-status Report
  • [Presentation] サイトカイニンはイネのROLバリア形成に関与する2019

    • Author(s)
      清水香那, 塩野克宏
    • Organizer
      第50回記念根研究集会
    • Related Report
      2019 Research-status Report
  • [Remarks] 福井県立大学生物資源学部環境植物学分野HP

    • URL

      https://www.s.fpu.ac.jp/kankyo/Home/index.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 福井県立大学生物資源学部生物資源学科 環境植物学分野HP

    • URL

      http://www.s.fpu.ac.jp/kankyo/Home/research.html

    • Related Report
      2020 Research-status Report
  • [Remarks] 研究室HP

    • URL

      http://www.s.fpu.ac.jp/kankyo/Home/research.html

    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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