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Elucidation of the esterification mechanism of beta-cryptoxanthin in citrus fruit

Research Project

Project/Area Number 19K06015
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionShizuoka University

Principal Investigator

Zhang Lancui  静岡大学, 農学部, 特任助教 (20767371)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsβ-クリプトキサンチン / キサントフィル / エステル化 / β-Cryptoxanthin / β-cryptoxanthin / カンキツ / カロテノイド
Outline of Research at the Start

カンキツ品種のうち、果実にキサントフィルを高濃度に蓄積するものは、キサントフィルと脂肪酸がエステル化されている。特に、ウンシュウミカンではキサントフィルの一種であるβ-クリプトキサンチンが高濃度に含まれており、β-クリプトキサンチンのほとんどが脂肪酸とのエステル体として存在している。本研究課題では、β-クリプトキサンチンをエステル化する酵素遺伝子を探索、同定し、その機能を明らかにする。

Outline of Final Research Achievements

In this study, to elucidate the mechanism of the xanthophyll esterification in citrus fruit, the changes in the contents of xanthophyll esters were investigated in the flavedo and juice sacs of different citrus varieties during the ripening process. The results showed that the contents of xanthophyll esters increased gradually in citrus fruit during the ripening process, and more than 85% of xanthophylls were present in esterified forms in the mature fruit. In addition, we screed the genes involved in xanthophyll esterification by microarray analysis. The results showed that the expression of CitXES1, CitXES6, CitXES7, CitXES8, and CitXES9 increased rapidly in the citrus fruit during the ripening process. The changes in the expression of CitXESs were well consistent with the accumulation of xanthophyll esters during the ripening process, which indicated that CitXESs might be the key genes responsible for xanthophyll esterification in citrus fruit.

Academic Significance and Societal Importance of the Research Achievements

本申請課題で取り組むβ-クリプトキサンチンの脂肪酸とのエステル化は,β-クリプトキサンチンの高蓄積メカニズムに大きく関わっていることが予想される。本研究では,カンキツ果実における新たなβ-クリプトキサンチンの高蓄積機構を解明し,研究成果は機能性成分であるβ-クリプトキサンチンの高含有化栽培技術,貯蔵技術,高含有新品種の育種へ繋がる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (20 results)

All 2022 2021 2020

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (14 results) (of which Int'l Joint Research: 6 results) Book (2 results)

  • [Journal Article] Exogenous gibberellin induced regreening through the regulation of chlorophyll and carotenoid metabolism in Valencia oranges2022

    • Author(s)
      Nichapat Keawmanee, Gang Ma, Lancui Zhang, Masaki Yahata, Kan Murakami, Masashi Yamamoto, Nami Kojima, Masaya Kato
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 173 Pages: 14-24

    • DOI

      10.1016/j.plaphy.2022.01.021

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microarray analysis of carotenoid and chlorophyll biosynthetic gene expression in the citrus juice sacs irradiated with blue light2022

    • Author(s)
      Gang Ma, Lancui Zhang, Masaki Yahata, Kazuki Yamawaki, Takehiko Shimada, Hiroshi Fujii, Tomoko Endo, Masaya Kato.
    • Journal Title

      Acta Horticulturae

      Volume: 1337 Issue: 1337 Pages: 195-202

    • DOI

      10.17660/actahortic.2022.1337.26

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Accumulation of β-citraurin and expression of carotenoid cleavage dioxygenase4 gene in citrus fruit during maturation2022

    • Author(s)
      Miki Kato, Kohei Iida, Gang Ma, Lancui Zhang, Masaki Yahata and Masaya Kato
    • Journal Title

      Acta Horticulturae

      Volume: 1336 Issue: 1336 Pages: 61-66

    • DOI

      10.17660/actahortic.2022.1336.9

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Auxin induced carotenoid accumulation in GA and PDJ-treated citrus fruit after harvest2021

    • Author(s)
      Gang Ma, Lancui Zhang, Rin Kudaka, Hayato Inaba, Murakami Kan, Yamamoto Masashi, Kojima Nami, Masaki Yahata, Hikaru Matsumoto, Masaya Kato
    • Journal Title

      Postharvest Biology and Technology

      Volume: 181 Pages: 111676-111676

    • DOI

      10.1016/j.postharvbio.2021.111676

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] カンキツ果実におけるキサントフィルのエステル体含量の季節変動および関連遺伝子の発現解析2022

    • Author(s)
      山本将士, 馬 剛, 張嵐翠, 八幡昌紀, 島田武彦, 藤井浩, 遠藤朋子, 加藤雅也
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 培養した‘太田ポンカン’のフラベドにおけるカロテノイド,クロロフィルおよびジベレリン代謝に及ぼすジベレリンの影響2022

    • Author(s)
      望月春奈, 馬 剛, 張嵐翠, 八幡昌紀, 加藤雅也
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ‘山下紅早生’におけるβ-Citraurin代謝に関わるCarotenoid cleavage dioxygenase(CCD)遺伝子の発現および機能解析2022

    • Author(s)
      加藤美紀, 馬 剛, 張嵐翠, 八幡昌紀, 加藤雅也
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ウミカン果実における着色に及ぼすサリチル酸およびジャスモン酸の影響2022

    • Author(s)
      池谷将斗, 野村健太, 馬 剛, 張嵐翠, 八幡昌紀, 加藤雅也
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Characterization of β-cryptoxanthin esters accumulation and carotenoid biosynthetic genes expression during citrus fruit maturation2021

    • Author(s)
      Masashi Yamamoto, Gang Ma, Lancui Zhang, Masaki Yahata, Terutaka Yoshioka, Masaya Kato
    • Organizer
      V Asia Symposium on Quality Management in Postharvest Systems (ISHS)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microarray analysis of carotenoid and chlorophyll biosynthetic gene expression in the citrus juice sacs irradiated with blue light2021

    • Author(s)
      Gang Ma, Lancui Zhang, Masaki Yahata, Kazuki Yamawaki, Takehiko Shimada, Hiroshi Fujii, Tomoko Endo, Masaya Kato
    • Organizer
      IX International Symposium on Light in Horticulture (ISHS)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of Pigments Accumulation in Valencia Orange During Regreening2021

    • Author(s)
      Nichapat Keawmanee, Gang Ma, Lancui Zhang, Masaki Yahata, Masaya Kato
    • Organizer
      V Asia Symposium on Quality Management in Postharvest Systems (ISHS)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Accumulation of β-citraurin and expression of carotenoid cleavage dioxygenase4 gene in citrus fruit during maturation2021

    • Author(s)
      Miki Kato, Gang Ma, Lancui Zhang, Masaki Yahata, Masaya Kato
    • Organizer
      V Asia Symposium on Quality Management in Postharvest Systems (ISHS)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of phytohormones on coloration of Satsuma mandarin fruit during long-term storage2021

    • Author(s)
      Kenta Nomura, Hayato Inaba, Gang Ma, Lancui Zhang, Masaki Yahata, Hikaru Matsumoto, Masaya Kato
    • Organizer
      V Asia Symposium on Quality Management in Postharvest Systems (ISHS)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of auxin and 1-MCP on carotenoid and chlorophyll metabolisms in postharvest Satsuma Mandarin fruit2021

    • Author(s)
      Kan Murakami, Gang Ma, Lancui Zhang, Masaki Yahata, Hikaru Matsumoto, Masaya Kato
    • Organizer
      V Asia Symposium on Quality Management in Postharvest Systems (ISHS)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] カンキツの培養したフラべドにおけるカロテノイド集積に及ぼすアブシジン酸受容体阻害剤の影響2021

    • Author(s)
      北村陽,馬 剛,張嵐翠,八幡昌紀,山脇和樹,竹内純,轟泰司,加藤 雅也
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ‘太田ポンカン’のフラベドにおけるカロテノイドおよびクロロフィル代謝に及ぼす植物ホルモンの影響2021

    • Author(s)
      北谷友梨佳,望月春奈,馬 剛, 張嵐翠,八幡昌紀,山 脇和樹,松本光,加藤 雅也
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] バレンシアオレンジのフラベドにおけるカロテノイドおよびクロロフィル代謝に及ぼす植物ホルモンの影響2021

    • Author(s)
      望月春奈,北谷友梨佳,馬 剛,張嵐翠,八幡昌紀,山脇和樹,加藤雅也
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] カンキツ果実におけるキサントフィルのエステル化に関わる酵素遺伝子の探索2020

    • Author(s)
      山本将士,馬 剛,張嵐翠,八幡昌紀,山脇和樹,吉岡照高,太田智,加藤雅也
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Research-status Report
  • [Book] 園芸利用学(第3章、 第12章 分担)2021

    • Author(s)
      加藤 雅也, 馬剛, 張嵐翠( 編集: 山内 直樹, 今堀 義洋)
    • Total Pages
      312
    • Publisher
      文永堂出版
    • ISBN
      9784830041426
    • Related Report
      2021 Annual Research Report
  • [Book] The Genus Citrus (Co-editors Manuel Talon, Marco Caruso, Fred Gmitter). Chapter 24:Citrus and Health, 495-511頁2020

    • Author(s)
      Gang Ma,Lancui Zhang,Minoru Sugiura,Masaya Kato
    • Total Pages
      538
    • Publisher
      Elsevier
    • ISBN
      9780128121634
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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