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Does plant immunity have roles in chlorosis and cell death in chlorosis development model plants?

Research Project

Project/Area Number 19K06055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionEhime University

Principal Investigator

Kobayashi Kappei  愛媛大学, 農学研究科, 教授 (40244587)

Co-Investigator(Kenkyū-buntansha) 賀屋 秀隆  愛媛大学, 農学研究科, 准教授 (80398825)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords遺伝子組換え植物 / 細胞死 / 植物ウイルス / 植物免疫 / 退緑黄化 / 病徴 / ウイルス病 / 退緑 / 植物ホルモン / クロロシス / 植物ウイルス病 / シグナル伝達
Outline of Research at the Start

植物にもヒトのようにウイルスが引き起こす病害があり,多くの場合,クロロシスと呼ばれる葉の緑色が薄くなったり,黄色くなったりする症状が現れる.これまでの研究から,クロロシスの少なくとも一部では,植物が病原体から身を守るための「植物免疫」が不完全に働くために症状が激しくなっている可能性が考えられた.本研究は,クロロシスが現れる過程に植物免疫がどのように関わっているかを明らかにし,ウイルスが増殖しても病気の症状を示さず,収穫が可能な新しい作物を作り出す基礎を固めようとするものである.

Outline of Final Research Achievements

It is frequently observed that diseased plant leaves which show chlorotic symptoms such as virally induced mosaic, senesce more rapidly than healthy plant leaves. Our previous study suggested that chlorosis development accompanies the activation of plant immunity. Here we tested a hypothesis that plant immunity would have a role in plant disease symptom development. Our comprehensive gene expression study revealed the activation of plant immunity-related gene including those having roles in cell death induction, activation of defense hormone salicylic acid pathways, the production of reactive oxygen species during chlorosis development.

Academic Significance and Societal Importance of the Research Achievements

新型コロナウイルス感染症の治療に免疫抑制効果のあるステロイドが用いられたことを例にとるまでもなく,ヒトなど動物の感染症ではその発症に宿主免疫が密接に関連している.本研究では,植物免疫が病原体の排除という本来の役割に加え,病害発症という負の働きもあることを示した.これによって,より持続的な農業生産に役立つ新たな品種の開発に向けて新たな戦略が加わるとともに,風邪薬のような対症療法的な農薬を開発する道も拓かれた.

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (11 results)

All 2022 2021 2020 2019

All Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (2 results) Book (1 results)

  • [Journal Article] Knockout of Tobacco Homologs of Arabidopsis Multi-Antibiotic Resistance 1 Gene Confers a Limited Resistance to Aminoglycoside Antibiotics.2022

    • Author(s)
      Hafizur Rahman, Chika Fukushima, Hidetaka Kaya, Takashi Yaeno, Kappei Kobayashi
    • Journal Title

      International journal of molecular sciences

      Volume: 23 Issue: 4 Pages: 2006-2006

    • DOI

      10.3390/ijms23042006

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] RACE1, a Japanese Blumeria graminis f. sp. hordei isolate, is capable of overcoming partially mlo-mediated penetration resistance in barley in an allele-specific manner.2021

    • Author(s)
      Takashi Yaeno, Miki Wahara, Mai Nagano, Hikaru Wanezaki, Hirotaka Toda, Hiroshi Inoue, Ayaka Eishima, Masamichi Nishiguchi, Hiroshi Hisano, Kappei Kobayashi, Kazuhiro Sato, Naoto Yamaoka
    • Journal Title

      PLOS ONE

      Volume: 16 Issue: 8 Pages: e0256574-e0256574

    • DOI

      10.1371/journal.pone.0256574

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Simple Heat Treatment Increases SpCas9-Mediated Mutation Efficiency in Arabidopsis2021

    • Author(s)
      Kurokawa Shuta、Rahman Hafizur、Yamanaka Naoshi、Ishizaki Chisato、Islam Shaikhul、Aiso Tsuyoshi、Hirata Shunya、Yamamoto Mayuka、Kobayashi Kappei、Kaya Hidetaka
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 11 Pages: 1676-1686

    • DOI

      10.1093/pcp/pcab123

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Impaired Expression of Chloroplast HSP90C Chaperone Activates Plant Defense Responses with a Possible Link to a Disease-Symptom-Like Phenotype2020

    • Author(s)
      Shaikhul Islam, Sachin Ashok Bhor, Keisuke Tanaka, Hikaru Sakamoto,Takashi Yaeno, Hidetaka Kaya and Kappei Kobayashi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 12 Pages: 4202-4202

    • DOI

      10.3390/ijms21124202

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptome Analysis Shows Activation of Stress and Defense Responses by Silencing of Chlorophyll Biosynthetic Enzyme CHLI in Transgenic Tobacco2020

    • Author(s)
      Shaikhul Islam, Sachin Ashok Bhor, Keisuke Tanaka, Hikaru Sakamoto,Takashi Yaeno, Hidetaka Kaya and Kappei Kobayashi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 19 Pages: 7044-7044

    • DOI

      10.3390/ijms21197044

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] High Humidity Causes Abnormalities in the Process of Appressorial Formation of Blumeria graminis f. sp. hordei.2020

    • Author(s)
      Koreyuki Sugai, Hiroshi Inoue, Chie Inoue, Mayuko Sato, Mayumi Wakazaki, Kappei Kobayashi, Masamichi Nishiguchi, Kiminori Toyooka, Naoto Yamaoka, Takashi Yaeno
    • Journal Title

      Pathogens

      Volume: 9 Issue: 1 Pages: 45-45

    • DOI

      10.3390/pathogens9010045

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Complete genome sequence and phylogenetic relationships of tobacco streak virus causing groundnut stem necrosis disease in India.2019

    • Author(s)
      Daliyamola, Jailani, A.A.K. Vemana, K., Roy, A., Krishnareddy, M., Kobayashi, K. Mandal, B.
    • Journal Title

      VirusDisease

      Volume: 30 Issue: 2 Pages: 227-236

    • DOI

      10.1007/s13337-018-0500-2

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Formvar membrane laid on artificial medium induces haustorium-like structure formation in powdery mildew fungi2019

    • Author(s)
      Naoto Yamaoka, Eiji Tanaka, Tsubasa Ogasahara, Honoka Tani, Kappei Kobayashi, Takashi Yaeno
    • Journal Title

      Mycoscience

      Volume: 60 Issue: 5 Pages: 298-301

    • DOI

      10.1016/j.myc.2019.06.006

    • NAID

      40022012811

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Rapid activation of salicylic acid and systemic acquired resistance pathways by induced knock-down of HSP90C2022

    • Author(s)
      Unung, O. O., Mori, I., Goda, H., Fujita, Y., Kaya, H. and Kobayashi, K.
    • Organizer
      令和4年度日本植物病理学会関西部会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Two Different Pathways Activate Plant Immune-like Responses that lead to chlorosis development in Transgenic Tobacco Models2020

    • Author(s)
      Islam S, Tanaka K, Sakamoto H, Yaeno T, Kobayashi K
    • Organizer
      令和2年度日本植物病理学会大会
    • Related Report
      2019 Research-status Report
  • [Book] Antiviral Resistance in Plants2019

    • Author(s)
      Kobayashi K, Nishiguchi M (Eds.)
    • Total Pages
      292
    • Publisher
      Humana Press
    • ISBN
      9781493996353
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2024-01-30  

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