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Regulation of tissue-specific nonhost resistance by the circadian clock in plants

Research Project

Project/Area Number 19K06057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionFukui Prefectural University

Principal Investigator

Ishikawa Atsushi  福井県立大学, 生物資源学部, 教授 (70264687)

Co-Investigator(Kenkyū-buntansha) 仲下 英雄  福井県立大学, 生物資源学部, 教授 (70280724)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Discontinued (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsシロイヌナズナ / イネいもち病菌 / 非宿主抵抗性 / 組織特異性 / 概日時計 / CCA1 / 時間特異性
Outline of Research at the Start

植物の概日時計システムは、外部環境の変化に応答して時刻を調節する「入力系」、その情報を処理する概日時計、そして時刻情報をさまざまな生理現象として表現する「出力系」により構成されており、組織特異的にさまざまな生理応答を制御している。
しかし、非宿主抵抗性を制御する概日時計システムの組織特異性ならびに「入力系」および「出力系」については、ほとんどわかっていない。
本研究では、シロイヌナズナの概日時計による組織特異的な非宿主抵抗性制御機構を概日時計と「出力系」との関連から明らかにしていく。

Outline of Final Research Achievements

Nonhost resistance (NHR) is the most robust and durable resistance in plants, but its spatiotemporal regulation is poorly understood. The circadian clock functions in a tissue-specific manner and regulates individual physiological processes in plants. Using mutant and RNA-seq analyses, we revealed a role of CCA1 in tissue-specific and time-of-day-specific regulation of NHR to Pyricularia oryzae in Arabidopsis. Targeted perturbation of CCA1 function in epidermis compromised time-of-day-specific regulation of NHR to P. oryzae in Arabidopsis. RNA-seq analysis showed that P. oryzae inoculation alters the transcriptome in pen2 plants and identified PMR5 as a candidate gene of direct targets of CCA1. Time-of-day-specific penetration resistance to P. oryzae was reduced in Arabidopsis pen2 pmr5 mutant plants. These findings suggest that epidermal CCA1 and PMR5 contribute to the establishment of time-of-day-specific NHR to P. oryzae in Arabidopsis.

Academic Significance and Societal Importance of the Research Achievements

本研究により、シロイヌナズナのイネいもち病菌に対する非宿主抵抗性においては、概日時計CCA1が葉の表皮細胞特異的にPMR5 遺伝子の発現を制御することにより非宿主抵抗性を組織特異的かつ時間特異的に制御していることが示唆された。この成果は、時刻および植物組織を選んで植物防除の効果を最大限引き出す技術(組織特異的な概日時計を標的とした農薬の開発および遺伝子組み換え作物の創出等)の開発につながると期待される。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (9 results)

All 2021 2020 2019 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (3 results) Remarks (2 results)

  • [Journal Article] Photoperiod Following Inoculation of Arabidopsis with Pyricularia oryzae (syn. Magnaporthe oryzae) Influences on the Plant?Pathogen Interaction2021

    • Author(s)
      Shimizu Sayaka、Yamauchi Yuri、Ishikawa Atsushi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 9 Pages: 5004-5004

    • DOI

      10.3390/ijms22095004

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tryptophan-derived metabolites and BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED RECEPTOR KINASE 1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola2021

    • Author(s)
      Ayumi Kosaka, Marta Pastorczyk, Mariola Pislewska-Bednarek, Takumi Nishiuchi, Erika Ono, Haruka Suemoto, Atsushi Ishikawa, Henning Frerigmann, Masanori Kaido, Kazuyuki Mise, Pawel Bednarek, Yoshitaka Takano
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 1488-1488

    • DOI

      10.1038/s41598-020-79562-x

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] CCA1 and LHY contribute to nonhost resistance to Pyricularia oryzae (syn. Magnaporthe oryzae) in Arabidopsis thaliana.2020

    • Author(s)
      Yamaura S, Yamauchi Y, Makihara M, Yamashino T, Ishikawa A.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 84 Issue: 1 Pages: 76-84

    • DOI

      10.1080/09168451.2019.1660612

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] bak1-5 mutation uncouples tryptophan-dependent and independent postinvasive immune pathways triggered in Arabidopsis by multiple fungal pathogens2020

    • Author(s)
      Ayumi Kosaka, Marta Pastorczyk, Mariola Pislewska-Bednarek, Takumi Nishiuchi, Haruka Suemoto, Atsushi Ishikawa, Henning Frerigmann, Masanori Kaido, Kazuyuki Mise, Pawel Bednarek, Yoshitaka Takano
    • Journal Title

      bioRxiv

      Volume: none

    • DOI

      10.1101/2020.04.26.052480

    • Related Report
      2019 Research-status Report
    • Open Access
  • [Presentation] イネいもち病菌接種後の日照条件はシロイヌナズナーイネいもち病菌相互作用に影響を及ぼす2021

    • Author(s)
      清水紗也佳,山内友里,石川敦司
    • Organizer
      令和3年度日本植物病理学会関西部会
    • Related Report
      2021 Annual Research Report
  • [Presentation] シロイヌナズナのイネいもち病菌に対する非宿主抵抗性におけるCCA1とLHYの関与2020

    • Author(s)
      山浦沙彩,山内友里,槇原基,山篠貴史,石川敦司
    • Organizer
      日本植物病理学会関西部会
    • Related Report
      2020 Research-status Report
  • [Presentation] シロイヌナズナのイネいもち病菌に対する非宿主抵抗性における概日時計の解析2019

    • Author(s)
      野口崇哉, 石川敦司
    • Organizer
      令和元年度北陸線植物バイオサイエンス研究会
    • Related Report
      2019 Research-status Report
  • [Remarks] 福井県立大学生物資源学部生物資源学科植物病理学分野

    • URL

      https://www.s.fpu.ac.jp/fpu-bio/works5F-2.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 福井県立大学生物資源学部分子生物学研究領域

    • URL

      http://www.fpu.ac.jp/biotech/kenkyu/5f.html

    • Related Report
      2020 Research-status Report

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Published: 2019-04-18   Modified: 2024-01-30  

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