• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

For control of nematodes with nematodes: development of a new pine wilt control technology using predatory nematodes

Research Project

Project/Area Number 19K06145
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40010:Forest science-related
Research InstitutionKyoto University

Principal Investigator

Takeuchi-Kaneko Yuko  京都大学, 地球環境学堂, 助教 (80452283)

Co-Investigator(Kenkyū-buntansha) 神崎 菜摘  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (70435585)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsマツ材線虫病 / マツノザイセンチュウ / 捕食性線虫 / 生物的防除 / 生物防除
Outline of Research at the Start

病原線虫マツノザイセンチュウが媒介昆虫によってマツ属樹木に導入されることで起こるマツ材線虫病は、白砂青松を擁する日本古来の景観を壊滅状態に追い込み、今なお拡大を続けている。本病は感染後の致死率が高い上に伝播力が極めて強いため一旦被害が発生すると制御することは難しく、有効な防除策の確立が急務である。本研究では、マツノザイセンチュウと同じAphelenchoididae科に属する近縁種Seinura cavernaのもつ高い捕食能に着目し、線虫における捕食行動研究のモデル化に必要な基礎情報を集積するとともに、マツノザイセンチュウの個体群抑制技術に基づく本病の新規防除法開発を目指す。

Outline of Final Research Achievements

Seinura caverna, a species closely related to the pine wood nematode, the causative agent of pine wilt disease, is a hermaphroditic species with a high ability to prey on nematodes. As a result of comparing and observing the ultrastructure of several closely related Aphelenchoididae species with diverse life histories, we found that S. caverna and its related predatory nematode share a highly elastic cuticle structure. It is speculated that this functions as a physical signal that distinguishes preys from others when ejecting the stylet for predation. In addition, S. caverna significantly suppressed the population of the pine wood nematodes in vitro, suggesting its potential as a biological control material.

Academic Significance and Societal Importance of the Research Achievements

捕食性線虫でありながら培養が容易であり単一個体から増殖できるというS. cavernaがもつ実験材料としてのアドバンテージを生かし、同種を線虫における捕食行動研究モデルとするための基礎的知見を集積し、研究基盤を整えた。また対峙培養によりマツノザイセンチュウに対する個体数抑制効果を確認し、生物防除資材としての利用可能性を示した。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (27 results)

All 2023 2022 2021 2020 2019

All Journal Article (26 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 26 results,  Open Access: 12 results) Book (1 results)

  • [Journal Article] Onthodiplogaster japonica n. gen., n. sp. (Rhabditida: Diplogastridae) isolated from Onthophagus sp. (Coleoptera: Scarabaeidae) from Japan2023

    • Author(s)
      Kanzaki Natsumi、Ikeda Yuya、Shinya Ryoji
    • Journal Title

      Scientific Reports

      Volume: 13 Issue: 1 Pages: 6470-6470

    • DOI

      10.1038/s41598-023-33586-1

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nematode and Strepsipteran Parasitism in Bait-Trapped and Hand-Collected Hornets (Hymenoptera, Vespidae, Vespa)2023

    • Author(s)
      Kanzaki Natsumi、Makino Shun’ichi、Kosaka Hajime、Sayama Katsuhiko、Hamaguchi Keiko、Narayama Shinji
    • Journal Title

      Insects

      Volume: 14 Issue: 4 Pages: 398-398

    • DOI

      10.3390/insects14040398

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultrastructural study of anther parasitism of Ficus laevigata by Ficophagus laevigatus (Aphelenchoididae)2023

    • Author(s)
      Giblin-Davis Robin M.、Kanzaki Natsumi、Williams Donna S.
    • Journal Title

      Journal of Nematology

      Volume: 55 Issue: 1

    • DOI

      10.2478/jofnem-2023-0010

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Schistonchus pumilae n. sp. isolated from syconia of a creeper fig, Ficus pumila L. from the Japanese warm temperate zone2023

    • Author(s)
      Kanzaki Natsumi、Tanaka Ryusei、Giblin-Davis Robin M.
    • Journal Title

      Nematology

      Volume: 25 Issue: 5 Pages: 509-529

    • DOI

      10.1163/15685411-bja10236

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] On the phylogenetic status of Contortylenchus genitalicola Kosaka?& Ogura, 19932023

    • Author(s)
      Kanzaki Natsumi、Kosaka Hajime、Fujimori Yuta、Akiba Mitsuteru
    • Journal Title

      Nematology

      Volume: 25 Issue: 5 Pages: 1-6

    • DOI

      10.1163/15685411-bja10233

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The developmental and structural uniqueness of the embryo of the extremophile viviparous nematode, Tokorhabditis tufae2023

    • Author(s)
      Yamashita Tatsuya、Ekino Taisuke、Kanzaki Natsumi、Shinya Ryoji
    • Journal Title

      Frontiers in Physiology

      Volume: 14 Pages: 1197477-1197477

    • DOI

      10.3389/fphys.2023.1197477

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] First record of Stigmatomyces (Ascomycota: Laboulbeniales) on Drosophilidae from Japan2023

    • Author(s)
      Yamazaki Izumi、Onuma Moe、Omiya Haruka、Ri Tomohiko、Kanzaki Natsumi、Degawa Yousuke、Sawamura Kyoichi
    • Journal Title

      Fly

      Volume: 17 Issue: 1

    • DOI

      10.1080/19336934.2023.2234265

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bursaphelenchus ogawanus n. sp. isolated from bark material of Fagus japonica Maxim. from Japan2023

    • Author(s)
      Kanzaki Natsumi、Masuya Hayato
    • Journal Title

      Nematology

      Volume: 25 Issue: 7 Pages: 729-747

    • DOI

      10.1163/15685411-bja10252

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highlighting potential physical and chemical cues involved in conspecific recognition system in a predator nematode, Seinura caverna2023

    • Author(s)
      Ekino Taisuke、Yoshiga Toyoshi、Takeuchi-Kaneko Yuko、Ichihara Yu、Kanzaki Natsumi、Shinya Ryoji
    • Journal Title

      Integrative And Comparative Biology

      Volume: 63 Issue: 4 Pages: 865-876

    • DOI

      10.1093/icb/icad105

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Laboratory experiments of Stigmatomyces majewskii (Laboulbeniales: Laboulbeniaceae) infection on Drosophila suzukii (Diptera: Drosophilidae)2023

    • Author(s)
      Yamazaki Izumi、Onuma Moe、Ri Tomohiko、Okane Izumi、Kanzaki Natsumi、Degawa Yousuke、Sawamura Kyoichi
    • Journal Title

      Applied Entomology and Zoology

      Volume: 58 Issue: 4 Pages: 379-385

    • DOI

      10.1007/s13355-023-00843-8

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sachsia putridicola n. sp. (Rhabditida: Diplogastridae), isolated from an Onthophagus dung beetle (Coleoptera: Scarabaeidae) from Tsukuba, Japan2023

    • Author(s)
      Kanzaki Natsumi、Degawa Yousuke
    • Journal Title

      Nematology

      Volume: 25 Issue: 10 Pages: 1141-1156

    • DOI

      10.1163/15685411-bja10282

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bursaphelenchus suri n. sp.: A second Bursaphelenchus syconial parasite of figs supports adaptive radiation among section Sycomorus figs2022

    • Author(s)
      Kanzaki Natsumi、Kruger Meike S.、Greeff Jaco M.、Giblin-Davis Robin M.
    • Journal Title

      PLOS ONE

      Volume: 17 Issue: 4 Pages: e0265339-e0265339

    • DOI

      10.1371/journal.pone.0265339

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparison of geographical isolates of Bursaphelenchus tadamiensis (Tylenchomorpha: Aphelenchoididae) with remarks on its preferred habitat2022

    • Author(s)
      Kanzaki Natsumi、Hamaguchi Keiko、Ide Tatsuya
    • Journal Title

      Nematology

      Volume: 24 Issue: 7 Pages: 809-819

    • DOI

      10.1163/15685411-bja10169

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Transfer of four isolates of Bursaphelenchus doui into Monochamus alternatus and potential vector switching of the?nematode2022

    • Author(s)
      Maehara Noritoshi、Aikawa Takuya、Kanzaki Natsumi、Nakamura Katsunori
    • Journal Title

      Nematology

      Volume: 24 Issue: 8 Pages: 855-861

    • DOI

      10.1163/15685411-bja10173

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Predation drives convergent evolution of the thick and baggy cuticle in nematodes2022

    • Author(s)
      Ichiishi Kanata、Ekino Taisuke、Kanzaki Natsumi、Shinya Ryoji
    • Journal Title

      Nematology

      Volume: 24 Pages: 1-8

    • DOI

      10.1163/15685411-bja10196

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Description of Ruehmaphelenchus fujiensis n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from dead wood of Quercus crispula from Yamanashi, Japan2021

    • Author(s)
      Kanzaki Natsumi
    • Journal Title

      Nematology

      Volume: 23 Issue: 7 Pages: 725-742

    • DOI

      10.1163/15685411-bja10072

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Description of Bursaphelenchus microcarpae n. sp. isolated from Cryphalus abbreviatus emerged from Ficus microcarpa, with a report of B. carpini re-isolation2021

    • Author(s)
      Kanzaki Natsumi、Ekino Taisuke、Kajimura Hisashi、Degawa Yousuke
    • Journal Title

      Nematology

      Volume: 23 Issue: 7 Pages: 743-759

    • DOI

      10.1163/15685411-bja10073

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nematode isolation from nitidulid beetles with proposal of Sheraphelenchus heterophallus n. comb.2021

    • Author(s)
      Kanzaki Natsumi
    • Journal Title

      Nematology

      Volume: 23 Issue: 8 Pages: 897-908

    • DOI

      10.1163/15685411-bja10084

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Description of Basilaphelenchus pedrami n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from Kyoto, Japan2021

    • Author(s)
      Kanzaki Natsumi
    • Journal Title

      Nematology

      Volume: 23 Issue: 7 Pages: 803-816

    • DOI

      10.1163/15685411-bja10078

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Description of Aphelenchoides roulingae n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from Xylocopa collaris sauteri collected from Yilan, Taiwan, with some notes on A. xylocopae Kanzaki, 20062021

    • Author(s)
      Kanzaki Natsumi、Li Hou-Feng、Ekino Taisuke、Takeuchi-Kaneko Yuko、Maehara Noritoshi、Aikawa Takuya
    • Journal Title

      Nematology

      Volume: 23 Issue: 10 Pages: 1093-1107

    • DOI

      10.1163/15685411-bja10097

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Three Seinura species from Japan with a description of S. shigaensis n. sp. (Tylenchomorpha: Aphelenchoididae)2021

    • Author(s)
      Kanzaki Natsumi、Ekino Taisuke、Hamaguchi Keiko、Takeuchi-Kaneko Yuko
    • Journal Title

      PLOS ONE

      Volume: 16 Issue: 1 Pages: e0244653-e0244653

    • DOI

      10.1371/journal.pone.0244653

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chylorhabditis epuraeae n. gen., n. sp. (Rhabditida: Rhabditidae) isolated from Epuraea (Haptoncus) ocularis Fairmaire collected from sap on the bark of Ulmus parvifolia Jacq. in Kyoto, Japan2021

    • Author(s)
      Kanzaki Natsumi、Hamaguchi Keiko、Takeuchi-Kaneko Yuko
    • Journal Title

      Nematology

      Volume: 23 Issue: 4 Pages: 1-15

    • DOI

      10.1163/15685411-bja10051

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparative Secretome and Functional Analyses Reveal Glycoside Hydrolase Family 30 and Cysteine Peptidase as Virulence Determinants in the Pinewood Nematode Bursaphelenchus xylophilus2021

    • Author(s)
      Shinya Ryoji、Kirino Haru、Morisaka Hironobu、Takeuchi-Kaneko Yuko、Futai Kazuyoshi、Ueda Mitsuyoshi
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 640459-640459

    • DOI

      10.3389/fpls.2021.640459

    • NAID

      120006975989

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The genus Berntsenus Massey, 1974 is a junior synonym of Bursaphelenchus Fuchs, 19372020

    • Author(s)
      Kanzaki Natsumi、Giblin-Davis Robin M.
    • Journal Title

      Nematology

      Volume: 22 Issue: 6 Pages: 677-695

    • DOI

      10.1163/15685411-00003332

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lenisaphelenchus ulomae n. gen., n. sp. (Rhabditida: Aphelenchoididae) isolated from the body cavity of Uloma marseuli Nakane (Coleoptera: Tenebrionidae) from Japan2020

    • Author(s)
      Kanzaki Natsumi、Hamaguchi Keiko
    • Journal Title

      Nematology

      Volume: 22 Issue: 9 Pages: 1-14

    • DOI

      10.1163/15685411-bja10004

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bursaphelenchus acaloleptae n. sp. sharing tree and beetle carrier hosts with B. luxuriosae Kanzaki & Futai, 2003 in Japan2019

    • Author(s)
      Kanzaki Natsumi、Ekino Taisuke、Maehara Noritoshi、Aikawa Takuya、Giblin-Davis Robin M.
    • Journal Title

      Nematology

      Volume: 22 Issue: 5 Pages: 1-13

    • DOI

      10.1163/15685411-00003320

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Book] 木本植物の被食防衛2023

    • Author(s)
      小池 孝良、塩尻 かおり、中村 誠宏、鎌田 直人
    • Total Pages
      280
    • Publisher
      共立出版
    • ISBN
      9784320058408
    • Related Report
      2022 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi