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Histological observation of gonadal sex differentiation in the cartilaginous fish, the red stingray (Hemitrygon akajei).

Research Project

Project/Area Number 19K06229
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionKindai University

Principal Investigator

Kobayashi Yasuhisa  近畿大学, 農学部, 准教授 (10643786)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsアカエイ / 軟骨魚類 / 性分化 / 卵巣
Outline of Research at the Start

サメやエイは、それらを知らない人間がいないほどメジャーな存在である。しかしサメ/エイが属する軟骨魚類の生理機構に本格的に切り込んだ研究は、国内外を見渡しても限られている。本研究では、胎仔を母胎内の子宮にて養育する卵胎生の繁殖様式を持つアカエイを用いる。具体的にはアカエイの子宮内で発達する胎仔を用いて、発生に伴う生殖腺や外部生殖器の性分化過程を組織/分子学的手法を用いて解析する。これらの解析を通じて、軟骨魚類も哺乳類と同様に「子宮内」にて個体の性分化が起きているのかを明らかにする。

Outline of Final Research Achievements

The red stingray (Hemitrygon akajei), relatively easy to collect among cartilaginous fishes, have an ovoviviparous reproductive system. In this stingray, the gonadal sex determination and differentiation of the embryo exposed to maternal estrogen have occurred in the uterus. In this study, we investigated the process of gonadal sex differentiation of red stingray embryos. Histological observations revealed that morphological sex difference of the gonads occurs in the embryo, which is about 2 cm in total length. These findings help to understand the reproduction of cartilaginous fish.

Academic Significance and Societal Importance of the Research Achievements

サメやエイが含まれる軟骨魚類は、非常にメジャーな存在である。しかし実験サンプルの確保が極めて困難なため、軟骨魚類に関する研究は少ない。そのため軟骨魚類の資源保護のために重要となる繁殖に関する基礎的な情報が不足している。本研究では哺乳類と同様に、母体子宮内で性分化/性決定を行うアカエイを用いて、子宮内胎仔の生殖腺の性分化過程を組織学的に解析した。その結果、アカエイの生殖腺の性分化が起こる胎仔の発育段階と、その形態的特徴を明らかにした。 このような軟骨魚類の基礎的な知見を収集することにより、脊椎動物全般の性決定/性分化様式の多様性を理解する事に繋がると考えられる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (9 results)

All 2022 2021 2020

All Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Book (2 results)

  • [Journal Article] Vasopressin-oxytocin?type signaling is ancient and has a conserved water homeostasis role in euryhaline marine planarians2022

    • Author(s)
      Kobayashi Aoshi、Hamada Mayuko、Yoshida Masa-aki、Kobayashi Yasuhisa、Tsutsui Naoaki、Sekiguchi Toshio、Matsukawa Yuta、Maejima Sho、Gingell Joseph J.、Sekiguchi Shoko、Hamamoto Ayumu、Hay Debbie L.、Morris John F.、Sakamoto Tatsuya、Sakamoto Hirotaka
    • Journal Title

      Science Advances

      Volume: 8 Issue: 9 Pages: 0311-0311

    • DOI

      10.1126/sciadv.abk0331

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of synthetic androgen treatment on the gonad of juvenile longtooth grouper (Epinephelus bruneus)2021

    • Author(s)
      Yasuhisa Kobayashi、Takamasa Morishita、Hisashi Chuda
    • Journal Title

      International Aquatic Research

      Volume: 13 Pages: 163-163

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus2021

    • Author(s)
      Hirooka Asuka、Hamada Mayuko、Fujiyama Daiki、Takanami Keiko、Kobayashi Yasuhisa、Oti Takumi、Katayama Yukitoshi、Sakamoto Tatsuya、Sakamoto Hirotaka
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 13315-13315

    • DOI

      10.1038/s41598-021-92528-x

    • NAID

      120007120048

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Testicular inducing steroidogenic cells trigger sex change in groupers2021

    • Author(s)
      Murata Ryosuke、Nozu Ryo、Mushirobira Yuji、Amagai Takafumi、Fushimi Jun、Kobayashi Yasuhisa、Soyano Kiyoshi、Nagahama Yoshitaka、Nakamura Masaru
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 11117-11117

    • DOI

      10.1038/s41598-021-90691-9

    • NAID

      120007166819

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Eats a lot but poops nothing: embryonic mechanisms to maintain a clean uterine environment in a viviparous stingray2020

    • Author(s)
      Taketeru Tomita, Masaru Nakamura, Yasuhisa Kobayashi, Atsushi Yoshinaka, Kiyomi Murakumo
    • Journal Title

      Scientific Reports

      Volume: -

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Shift of the Vegetal Pole Area of Full-Grown Oocytes Toward the Ovulatory Site of the Ovary in the Medaka Fish, Oryzias latipes (Beloniformes: Adrianichthyidae).2020

    • Author(s)
      Takashi Iwamatsu, Shoji Oda, Hirokuni Kobayashi, Lynne R. Parenti, Yasuhisa Kobayashi.
    • Journal Title

      The Biological Bulletin.

      Volume: -

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] ハタ科魚類の性分化と性転換に関する形態学的および生理学的研究.2020

    • Author(s)
      村田良介, 小林靖尚, 野津了, 中村將
    • Journal Title

      日本水産学会誌

      Volume: -

    • NAID

      130007884617

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Book] 第7章 軟骨魚類の繁殖と性分化 [e-水産学シリーズ2: 魚類の性決定・性分化・性転換-これまでとこれから]2021

    • Author(s)
      小林靖尚、野津了、中村將
    • Total Pages
      242
    • Publisher
      恒星社厚生閣
    • Related Report
      2020 Research-status Report
  • [Book] 第8章 魚類の性転換 [e-水産学シリーズ2: 魚類の性決定・性分化・性転換-これまでとこれから]2021

    • Author(s)
      野津了、小林靖尚、中村將
    • Total Pages
      242
    • Publisher
      恒星社厚生閣
    • Related Report
      2020 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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