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Study of the biological significance of oligosaccharide nucleotides separated from domestic farm animals' milk and attempt to utilize it in animal feed

Research Project

Project/Area Number 19K06364
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42010:Animal production science-related
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

Urashima Tadasu  帯広畜産大学, 畜産学部, 教授 (80185082)

Co-Investigator(Kenkyū-buntansha) 福田 健二  帯広畜産大学, 畜産学部, 教授 (80419217)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords糖ヌクレオチド / 酪農家畜 / 初乳 / 腸管バリア機能 / MUC2 / MUC3 / オリゴ糖ヌクレオチド / 酪農家畜初乳 / UDP-Glc / UDP-Gal / 腸管ムチン / 発現調節 / 家畜 / 上皮細胞 / 増殖刺激 / 免疫調整 / ウシ初乳 / ヤギ初乳 / ヒツジ初乳 / 糖リン酸 / ヌクレオチド / 上皮細胞増殖促進 / 乳 / 生理機能
Outline of Research at the Start

ウシ, ヤギ, ヒツジの乳・初乳を採集し、そこから糖ヌクレオチドとオリゴ糖ヌクレオチドの分離方法を開発し、分離された成分の構造解析を行う。それらを腸管上皮細胞の培養系に添加し、細胞増殖と分化に対する刺激を測定するとともに添加によって発現が調整されたゲノム中の遺伝子を特定する。オリゴ糖ヌクレオチドの大量調製ならびに化学合成を試みるとともに、調製された成分をin vivo試験に供する。えられた結果に基づいて、飼料添加剤としての活用を検討する。

Outline of Final Research Achievements

Although the separation of oligosaccharide nucleotides from the colostra of domestic farm animals including, cow, goat and sheep, these were not separated. However, UDP-Gal, UDP-Glc, UDP-GalNAc and UDP-GlcNAc as sugar nucleotides were identified.
In in vitro experiment with intestinal epithelial cell, the growth stimulation had not been found by addition of UDP-Gal into the medium.To study the effect on intestinal barrier function by exposure with the sugar nucleotide, the mixed intestinal epithelial cell of Caco2:HT29-MTX cells =9 : 1, which was imitated for small intestine, was cultured with the final concentration of 200 μg/mL of UDP-Gal or UDP-Glc for 6, 12 or 24 hrs. Then total RNA was extracted from the cells and reverse - expressed to cDNA. The expressed levels of MUC2 or MUC3 was measured by real time PCR. As a result, the significant increase of the expression level for MUC2 was found in the cells, which were cultured with UDP-Gal for 6 hr.

Academic Significance and Societal Importance of the Research Achievements

オリゴ糖ヌクレオチドという機能未知の物質の普遍的な存在と機能解明を行うことは、生化学に新たな知見を切り拓く壮大なチャレンジであったが達成できなかった。一方、酪農家畜の初乳に含まれる糖ヌクレオチドの種類を、従来の方法ではなく、現代的な精密な手法で明らかにしたことは意義がある。また、細胞内での糖鎖合成材料としてではなく、分泌液である初乳に含まれる糖ヌクレオチドが、乳子の腸管通過中にバリア機能に影響しうる可能性のあるデータがえられたことは、機能解明への今後の展開に希望をいだかせるものである。そのような試みによってその機能が解明されれば、家畜飼料・食品添加素材としての利用可能性をもたらすであろう。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (13 results)

All 2022 2021 2020 2019

All Journal Article (10 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Evolution of milk oligosaccharides: Origin and selectivity of the ratio of milk oligosaccharides to lactose among mammals2022

    • Author(s)
      T. Urashima, T. Katayama, M. Sakanaka, K. Fukuda, M. Messer
    • Journal Title

      Biochim. Biophys. Acta General subject

      Volume: 1866 Issue: 1 Pages: 130012-130012

    • DOI

      10.1016/j.bbagen.2021.130012

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] In digenous oligosaccharides in bovine milk2022

    • Author(s)
      R. Hickey, T. Urashima
    • Journal Title

      Encyclopedia of Dairy Science, the third edition

      Volume: 3 Pages: 706-722

    • DOI

      10.1016/b978-0-12-818766-1.00322-6

    • ISBN
      9780128187678
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Journal Article] Diversification of a fucosyllactose transporter within the genus Bifidobacterium2022

    • Author(s)
      Ojima, M.N., Asao Y., Nakajima A., Katoh T., Kitaoka, M., Gotoh A., Hirose, J., Urashima, T., Fukiya, S., Yokota, A., Hachem M.B., Sakanaka M., Katayama T
    • Journal Title

      Appl. Environ. Micribiol.

      Volume: 88

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterisation of sugar nucleotides in colostrum of dairy domestic farms animals2021

    • Author(s)
      Mineguchi Yuri、Goto Kyosuke、Sudo Yuna、Hirayama Kentaro、Kashiwagi Hirotoshi、Sasagase Izumi、Kitazawa Haruki、Asakuma Sadaki、Fukuda Kenji、Urashima Tadasu
    • Journal Title

      International Dairy Journal

      Volume: 113 Pages: 104897-104897

    • DOI

      10.1016/j.idairyj.2020.104897

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Milk oligosaccharides2020

    • Author(s)
      浦島 匡、片山 高嶺、福田 健二
    • Journal Title

      生化学

      Volume: 92 Issue: 3 Pages: 307-322

    • DOI

      10.14952/SEIKAGAKU.2020.920307

    • ISSN
      0037-1017
    • Year and Date
      2020-06-25
    • Related Report
      2020 Research-status Report
  • [Journal Article] Evolution of Milk Oligosaccharides of Carnivora and Artiodactyla: Significance of the Ratio of Oligosaccharides to Lactose in Milk2020

    • Author(s)
      Urashima Tadasu、Mineguchi Yuri、Fukuda Kenji、Whitehouse-Tedd Katherine、Oftedal Olav T.
    • Journal Title

      Evolutionary Biology -A Transdisciplinary Approach

      Volume: 1 Pages: 359-377

    • DOI

      10.1007/978-3-030-57246-4_15

    • ISBN
      9783030572457, 9783030572464
    • Related Report
      2020 Research-status Report
  • [Journal Article] Chemical structures of oligosaccharides in milks of the American black bear (Ursus Americanus Americanus) and cheetah (Acinonyx jabatus)2020

    • Author(s)
      T. Urashima, M. Umewaki, E. Taufik, T. Ohshima, K. Fukuda, T. Saito, K. Whitehouse-Tedd, J.A. Budd, O.T. Oftedal
    • Journal Title

      Glycoconjugate Journal

      Volume: 37 Issue: 1 Pages: 57-76

    • DOI

      10.1007/s10719-019-09899-7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis2019

    • Author(s)
      M. Sakanaka, M.E. Hansen, A. Gotoh, T. Katoh, K. Yoshida, T. Odamaki, H. Yachi, Y. Sugiyama, S. Kurihara, J. Hirose, T. Urashima, J. Xiao, M. Kitaoka, S. Fukiya, A. Yokota, L.L. Leggio, M.A. Hachem, T. Katayama
    • Journal Title

      Science Advance

      Volume: 5 Issue: 8

    • DOI

      10.1126/sciadv.aaw7696

    • NAID

      120006716596

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Change of milk compositions in giraffe during the course of lactation2019

    • Author(s)
      河村あゆみ, 田中正之, 牛嶋隼也, 中井 壱, 峯口祐里, 福田健二, 浦島 匡
    • Journal Title

      Milk Science

      Volume: 68 Issue: 1 Pages: 44-48

    • DOI

      10.11465/milk.68.44

    • NAID

      130007638499

    • ISSN
      1343-0289, 2188-0700
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] ミルクオリゴ糖の機能研究における最近の進歩(4)2019

    • Author(s)
      浦島 匡、福田健二
    • Journal Title

      応用糖質科学

      Volume: 9 Pages: 254-265

    • NAID

      40022081186

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] Evolution of milk oligosaccharides in Carnivora and Artiodactyla2019

    • Author(s)
      T. Urashima, Y. Mineguchi, K. Fukuda
    • Organizer
      the 23th evolutionary biology meeting at Marseilles
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Chemical characterization of the milk oligosaccharides of some Artiodactyla species including giraffe (Giraffa camelopardalis), sitatunga (Tragelaphus spekii), deer (Cervus nippon yesoensis) and water buffalo (Bubalus bubalis)2019

    • Author(s)
      Y. Mineguchi, M. Miyoshi, E. Taufik, A. Kawamura, T. Asakawa, I. Suzuki, K. Souma, M. Okubo, T. Saito, K. Fukuda, S. Asakuma, T. Urashima
    • Organizer
      the 23th evolutionary biology meeting at Marseilles
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 酪農家畜の初乳における糖ヌクレオチドおよび糖1リン酸の分析2019

    • Author(s)
      峯口祐里、後藤京介、首藤悠那、柏木啓利、朝隈貞樹、北澤春樹、福田健二、浦島 匡
    • Organizer
      日本糖質学会年会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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