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Study on cytoadhesion mechanism of Babesia bovis-infected erythrocytes

Research Project

Project/Area Number 19K06384
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

Asada Masahito  帯広畜産大学, 原虫病研究センター, 准教授 (40587028)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsバベシア / 脳バベシア / ウシ / 赤血球 / ピロプラズマ / 脳 / 脳性バベシア
Outline of Research at the Start

脳性バベシア症はバベシア・ボビス(Babesia bovis)原虫感染によって引き起こされるウシの致死的な神経症状であるが、その分子機序は多重遺伝子にコードされた原虫のVESA-1タンパク質が関わるということ以外は不明である。本研究では脳性バベシア症の分子機序解明のため、申請者らが確立したバベシア原虫遺伝子組換え法を用い、接着に関わるVESA-1配列及び宿主側レセプターを同定する。

Outline of Final Research Achievements

Bovine babesiosis is a protozoan disease that causes significant economic loss to the livestock industry. Babesia bovis is the most virulent Babesia species and is known to cause a nervous symptom called “cerebral babesiosis” that is fatal to the host. In this study, we analyzed the molecular mechanism of cerebral babesiosis and identified VEAP as a molecule involved in infected erythrocytes’ adhesion to the bovine brain capillary endothelial cells, as well as several exported proteins including MTM. We also analyzed the sequence and adhesive properties of VESA-1, a molecule that is considered to be a ligand for adhesion.

Academic Significance and Societal Importance of the Research Achievements

本研究では脳性バベシア症における赤血球の細胞接着に関わる分子が新たに同定され、同症の分子機序の一端が明らかとなった。さらに、今回新たに同定されたVEAPやMTMは今後バベシア症に対する薬剤開発を行う上での標的分子となり得ると考えられる。さらに、今回得られた宿主細胞修飾分子やVESA-1の配列変化に関する新たな知見は、バベシア原虫にとどまらず、アピコンプレクサ門原虫の研究にも貢献するものと考えられる。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (9 results)

All 2022 2021 2020 2019

All Journal Article (8 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 8 results,  Open Access: 8 results) Book (1 results)

  • [Journal Article] Advances in understanding red blood cell modifications by Babesia2022

    • Author(s)
      Hakimi H, Yamagishi J, Kawazu SI, Asada M
    • Journal Title

      PLoS Pathogens

      Volume: 18(9) Issue: 9 Pages: e1010770-e1010770

    • DOI

      10.1371/journal.ppat.1010770

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation of viable Babesia bovis merozoites to study parasite invasion2021

    • Author(s)
      Hassan Hakimi, H, Masahito Asada, Takahiro Ishizaki, Shinichiro Kawazu
    • Journal Title

      Scientific reports

      Volume: 11 Issue: 1 Pages: 16959-16959

    • DOI

      10.1038/s41598-021-96365-w

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Recent Advances in Molecular Genetic Tools for Babesia2021

    • Author(s)
      Hassan Hakimi, H, Masahito Asada, Shinichiro Kawazu
    • Journal Title

      Veterinary Sciences

      Volume: 8 Issue: 10 Pages: 222-222

    • DOI

      10.3390/vetsci8100222

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Novel Babesia bovis exported proteins that modify properties of infected red blood cells2020

    • Author(s)
      Hakimi H, Templeton TJ, Sakaguchi M, Yamagishi J, Miyazaki S, Yahata K, Uchihashi T, Kawazu SI, Kaneko O, Asada M
    • Journal Title

      PLoS Pathogens

      Volume: 16(10) Issue: 10 Pages: e1008917-e1008917

    • DOI

      10.1371/journal.ppat.1008917

    • NAID

      120006988490

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ransient Transfection of the Zoonotic Parasite Babesia microti2020

    • Author(s)
      Liu M, Ji S, Rizk MA, Adjou Moumouni PF, Galon EM, Li J, Li Y, Zheng W, Benedicto B, Tumwebaze MA, Asada M, Xuan X
    • Journal Title

      Pathogens

      Volume: 9(2) Issue: 2 Pages: 108-108

    • DOI

      10.3390/pathogens9020108

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transient transfection of Babesia ovis using heterologous promoters2019

    • Author(s)
      Rosa C, Asada M, Hakimi H, Domingos A, Pimentel M, Antunes S
    • Journal Title

      Ticks Tick Borne Dis

      Volume: 10(6) Issue: 6 Pages: 101279-101279

    • DOI

      10.1016/j.ttbdis.2019.101279

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Initiated Babesia ovata Sexual Stages under In Vitro Conditions Were Recognized by Anti-CCp2 Antibodies, Showing Changes in the DNA Content by Imaging Flow Cytometry2019

    • Author(s)
      Nguyen TT, Dang-Trinh MA, Higuchi L, Mosqueda J, Hakimi H, Asada M, Yamagishi J, Umemiya-Shirafuji R, Kawazu SI
    • Journal Title

      Pathogens

      Volume: 8(3) Issue: 3 Pages: 104-104

    • DOI

      10.3390/pathogens8030104

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Epidemiology, risk factors, and co-infection of vector-borne pathogens in goats from Sistan and Baluchestan province, Iran2019

    • Author(s)
      Hakimi H, Sarani A, Takeda M, Kaneko O, Asada M
    • Journal Title

      PLoS One

      Volume: 14(6) Issue: 6 Pages: e0218609-e0218609

    • DOI

      10.1371/journal.pone.0218609

    • NAID

      120006987685

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Book] 寄生虫のはなし2020

    • Author(s)
      永宗喜三郎、脇 司、常盤俊大、島野智之編 (ピロプラズマの項目を麻田正仁、佐倉孝哉が執筆)
    • Total Pages
      168
    • Publisher
      朝倉書店
    • ISBN
      4254171749
    • Related Report
      2020 Research-status Report

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Published: 2019-04-18   Modified: 2024-01-30  

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