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Dynamics of RNA-protein complex in homologous chromosome pairing

Research Project

Project/Area Number 19K06503
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionNational Institute of Information and Communications Technology

Principal Investigator

Ding Daqiao  国立研究開発法人情報通信研究機構, 未来ICT研究所バイオICT研究室, 嘱託 (50359080)

Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords相同染色体対合 / 相分離 / 長鎖非コードRNA / RNA結合タンパク質 / in-vitro 解析 / 分裂酵母 / 減数分裂 / 液液相分離 / 染色体対合 / 精製タンパク質 / RNA / ドロップレット / 非コードRNA / 染色体 / 対合 / lncRNA
Outline of Research at the Start

有性生殖を司る減数分裂の最初のステップにおいで親世代から承け継いだ相同染色体が互いを見つけて対合するが、染色体対合のメカニズムが未だに解明されていない部分が多い。分裂酵母の染色体滞留lncRNAが相同染色体の対合を促進し、そこに複数のRNAプロセッシング因子が寄与することがこれまでの研究で明らかになった。本研究は、ライブセルイメージングからChIP-seqまで多様な分子細胞生物学研究手法を用い、新規のlncRNAに依存するロバストな対合サイトを同定し、さらにそのようなlncRNAの特徴を見出すことによって、染色体滞留lncRNAの相同染色体対合における役割と分子機構を明らかにしたい。

Outline of Final Research Achievements

Pairing of homologous chromosomes is a process in which homologous chromosomes inherited from parents find each other and adhere to each other. Normal pairing is essential for homologous recombination. The purpose of this research is to clarify the molecular mechanism of pairing. In this research, we found that long noncoding RNA loci contributed to pairing, and Smp proteins, which are multiple RNA-binding transcription termination factor, formed droplets with RNA through phase separation. We found that droplets with different RNAs could not fuse with each other, and only droplets with the same RNA could fuse with each other, which gave the specificity of homologous chromosome pairing. In addition, we reproduced droplet formation of Smp and RNA in vitro, and proved that physical properties of droplets were changed by the species of RNA.

Academic Significance and Societal Importance of the Research Achievements

減数分裂の最初のステップとして相同染色体が互いを見つけて対合しなければならないが、相同染色体対合のメカニズムが未だに解明されていない部分が多い。対合が失敗すると、その後の相同組換えが大きく下がり、正常の配偶子の形成に影響を及ぼす。人間の場合は、不妊症や流産を引き起こす可能性が高まる。従って、相同染色体対合のメカニズムの解明が生物学の基本問題であり、未解決である。本研究の研究成果は分裂酵母における対合のメカニズムの解明に貢献し、それは他の生物も類似する普遍的なメカニズムではないかを示唆した。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (17 results)

All 2022 2021 2020 2019 Other

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 7 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Rec8 Cohesin-mediated Axis-loop chromatin architecture is required for meiotic recombination2022

    • Author(s)
      Sakuno Takeshi、Tashiro Sanki、Tanizawa Hideki、Iwasaki Osamu、Ding Da-Qiao、Haraguchi Tokuko、Noma Ken-ichi、Hiraoka Yasushi
    • Journal Title

      Nucleic Acids Research

      Volume: - Issue: 7 Pages: 3799-3816

    • DOI

      10.1093/nar/gkac183

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Rec8 cohesin-mediated axis-loop chromatin architecture is required for meiotic recombination2021

    • Author(s)
      Sakuno Takeshi、Tashiro Sanki、Tanizawa Hideki、Iwasaki Osamu、Ding Da-Qiao、Haraguchi Tokuko、Noma Ken-ichi、Hiraoka Yasushi
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2021.12.09.472021

    • Related Report
      2021 Research-status Report
    • Open Access
  • [Journal Article] Linear elements are stable structures along the chromosome axis in fission yeast meiosis2021

    • Author(s)
      Ding Da-Qiao、Matsuda Atsushi、Okamasa Kasumi、Hiraoka Yasushi
    • Journal Title

      Chromosoma

      Volume: 2021 Issue: 2-3 Pages: 149-162

    • DOI

      10.1007/s00412-021-00757-w

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Linear Element is a Stable Structure Along the Chromosome Axis in Fission Yeast.2020

    • Author(s)
      Ding DQ, Matsuda A, Okamasa K, Hiraoka Y.
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2020.07.03.185785

    • Related Report
      2020 Research-status Report
    • Open Access
  • [Journal Article] Maintenance of meiotic crossover against reduced double-strand break formation in fission yeast lacking histone H2A.Z2020

    • Author(s)
      Yamada T, Yamada S, Ding DQ, Fujita Y, Takaya E, Hiraoka Y, Murakami H, Ohta K.
    • Journal Title

      Gene

      Volume: 743 Pages: 144615-144615

    • DOI

      10.1016/j.gene.2020.144615

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chromosome-associated RNA ;protein complexes promote pairing of homologous chromosomes during meiosis in Schizosaccharomyces pombe.2019

    • Author(s)
      Ding DQ, Okamasa K, Katou Y, Oya E, Nakayama J, Chikashige Y, Shirahige K, Haraguchi T, Hiraoka Y.
    • Journal Title

      Nature Communications

      Volume: 10 Issue: 1 Pages: 5598-5598

    • DOI

      10.1038/s41467-019-13609-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Histone H2A insufficiency causes chromosomal segregation defects due to anaphase chromosome bridge formation at rDNA repeats in fission yeast.2019

    • Author(s)
      Yamamoto TG, Ding DQ, Nagahama Y, Chikashige Y, Haraguchi T, Hiraoka Y.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 7159-7159

    • DOI

      10.1038/s41598-019-43633-5

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 減数分裂期相同染色体対合に寄与するlncRNA構造体2022

    • Author(s)
      丁大橋
    • Organizer
      日本分子生物学会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] lncRNA droplets mediate homologous chromosome pairing in meiosis2020

    • Author(s)
      丁 大橋
    • Organizer
      日本分子生物学会2020年会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Phase separation of RNA processing proteins is involved in homologous chromosome pairing during meiotic prophase2019

    • Author(s)
      丁大橋
    • Organizer
      10th International Meeting on Fission Yeast
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 相同染色体ペアリングにおける 液相分離の役割2019

    • Author(s)
      丁大橋
    • Organizer
      第71回日本細胞生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 分裂酵母における相同染色体認識のメカニズム2019

    • Author(s)
      丁大橋
    • Organizer
      酵母研究会第87回講演会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Book] 相分離生物学の全貌2020

    • Author(s)
      丁 大橋
    • Total Pages
      400
    • Publisher
      東京化学同人
    • ISBN
      9784807913466
    • Related Report
      2020 Research-status Report
  • [Remarks] 生物情報プロジェクト

    • URL

      https://www2.nict.go.jp/bio/seibutsu/CellMagic/index.html

    • Related Report
      2021 Research-status Report
  • [Remarks] 生物情報プロジェクト

    • URL

      https://www2.nict.go.jp/frontier/seibutsu/CellMagic/index.html

    • Related Report
      2020 Research-status Report
  • [Remarks] 情報通信研究機構研究報告 Vol.66 No.1 2020

    • URL

      http://www.nict.go.jp/publication/shuppan/kihou-journal/houkoku66-1/book/html5.html#page=31

    • Related Report
      2020 Research-status Report
  • [Remarks] 生命の遺伝情報継承に重要な相同染色体対合を促進する仕組みを発見

    • URL

      https://www.nict.go.jp/press/2019/12/10-1.html

    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2025-01-30  

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