| Project/Area Number |
19K06620
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 43050:Genome biology-related
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
MARLETAZ FERDINAND 沖縄科学技術大学院大学, 分子遺伝学ユニット, 客員研究員 (90833955)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | Cephalopod / limb / evolution / regulation / cephalopod / novelty / genes / transcription factors / development / Hox / chromatin / gene expression / regulatory profiling / limbs / evdevo / patterning / spiraling |
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| Outline of Research at the Start |
These experiments should show us which molecular actors are involved in limb patterning, they will show which new regulatory mechanisms have triggered the expression of Hox genes, and putative other actors, in limbs from their ancestral role in antero-posterior patterning. More generally, they will inform us on the way the genome is regulated in cephalopods, one of the most astonishing and complex group of animals outside vertebrates regulate their genome, and on the way they evolve novel complex morphological structure.
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| Outline of Final Research Achievements |
Our plan was to obtain RNA-seq and ATAC-seq library for each limb bud of a bobtail squid embryo in order to predict differences in transcription factor binding that would be involved in arm identity. We wanted to understand how a novel patterning could be deployed . We performed these experiments and sequenced corresponding libraries. We performed peak calling and differential peak activation analysis, which has been coupled with foot printing analysis. We identified some key genes and ordered corresponding probes for HCR, an advanced in situ hybridisation technique. In parellel, we optimised protocols for HCR imagining of squid embryos and fixed a lot of embryos. Some key results have been integrated in a paper about squid single-cell sequencing that will be submit in 2022.
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| Academic Significance and Societal Importance of the Research Achievements |
Development of new cephalopod model and new molecular biology techniques. Cephalopod have a strong cultural important (especially in Japan). Better understanding of biological novelties at regulatory level
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