| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In order to elucidate the mechanism by which specific G protein-coupled receptors localize to primary cilia, molecules that upregulate ciliary localization of serotonin receptor type 6 (Htr6) were explored. Yeast two-hybrid screening identified γ-adaptin as an interacting molecule of a region in the fourth intracellular domain of Htr6 that is important for ciliary localization of Htr6. By analysis of localization in hTERT-RPE1 cells knocked-out for γ-adaptin, it was suggested that localization of Htr6 to primary cilia is upregulated through the action of γ-adaptin on the fourth intracellular domain of Htr6.
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