Action of starfish gonadotropic hormone: interaction with the receptor and mechanism of gamete shedding
| Project/Area Number |
19K06747
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 44040:Morphology and anatomical structure-related
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 生殖腺刺激ホルモン / リラキシン様生殖巣刺激ペプチド / G-タンパク質共役受容体 / 生殖制御機構 / ヒトデ / リラキシン様生殖腺刺激ペプチド / 免疫組織化学染色 / C末アミド化ペプチド / Gタンパク質共役受容体 / 免疫組織化学 / シグナル伝達 / アセチルコリン |
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| Outline of Research at the Start |
生殖腺刺激ホルモン(GTH)は脊椎動物ばかりでなく無脊椎動物においても生殖細胞の形成や成長・成熟に重要な働きを持つ。ヒトデのGTHは放射神経に存在し、リラキシンとよく似た2本鎖ペプチドから構成されるペプチドホルモンである。それ故、ヒトデのGTHをリラキシン様生殖腺刺激ペプチド (relaxin-like gonad-stimulating peptide, RGP)と呼ぶ。本研究は、ヒトデをモデルに生殖制御機構を分子レベルで解明することを目的として、RGP受容体を同定し、RGPと受容体との相互作用の解析を行い、さらに、これまでほとんど未解決であった放卵・放精のメカニズムについて解析する。
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| Outline of Final Research Achievements |
Orthologs of relaxin-like gonadotrophic peptides (RGPs) were identified or predicted from 24 species of starfish. Some RGPs were chemically synthesized and examined spawning-inducing activities. RGP receptors (RGPRs) were also identified in Patiria pectinifera. The GPCRs were G protein-coupled receptors. Immunohistochemical analysis using anti-RGP antibodies revealed that RGP existed not only in the radial nerve cords and circumoral nerve rings but also in the terminal tentacles and gonoducts. Acetylcholine (ACh) was found within ovaries and testes. Thus presumably, RGP secreted from gonoducts stimulates ovarian follicle cells or interstitial cells, to produce maturation-inducing hormone, 1-methyladenine, resulting oocyte maturation and ACh secretion to shed mature eggs or spermatozoa.
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| Academic Significance and Societal Importance of the Research Achievements |
ヒトデのRGPは無脊椎動物で世界で最初に同定された生殖腺刺激ホルモン様活性物質でヒトの妊娠期の女性ホルモンとして知られるリラキシンとよく似た2本鎖ペプチドである。最近、RGPはヒトデ以外の他の棘皮動物（ナマコ類やクモヒトデ類）においても生殖腺刺激ホルモン様活性を持つことが明らかになった。さらにショウジョウバエの生殖巣の発達にリラキシン様ペプチド(Dilp8)とその受容体(Lgr3)が制御していることが示されている。それ故、本研究の成果は、ヒトデの生殖研究分野に新たな研究基盤を確立したばかりでなく、新口動物と旧口動物にまたがるリラキシン様ペプチドとその受容体の分子共進化を知る切っ掛けとなった。
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Report
(5 results)
Research Products
(39 results)
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[Presentation] Localisation of relaxin-like gonad-stimulating peptide expression in starfish: are the gonoducts the physiological source for its role as a regulator of spawning?2022
Author(s)
Feng, Y.L., Pinon Gonzalez, V.M., Lin, M., Egertova, M., Mita, M., and Elphick, M.R.
Organizer
30th Conference of the European Society for Comparative Endocrinology
Related Report
Int'l Joint Research
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[Presentation] Characterization and localization of relaxin-like gonad-stimulating peptide in the crown-of-thorns starfish, Acanthaster cf. solaris2022
Author(s)
Mita, M., Osugi, T., Tsuyoshi Kawada, T., Satake, H., Katayama, H., Kitamura, T., Miura, T., Miura, C.
Organizer
日本動物学会 第93回
Related Report
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[Presentation] Discovery of a second relaxin-like gonadotropic peptide in starfish2022
Author(s)
Mita, M., Katayama, H., Feng, Y., Pinon Gonzalez, V.M., Miyazaki, A., Elphick, M.R.
Organizer
The 95th Annual Meeting of the Japanese Biochemical Society
Related Report
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[Presentation] Metabolic regulation of sex differentiation under starvation in medaka2019
Author(s)
Sakae, Y., Sugiura, Y., Oikawa, A., Mita, M., Nishimura, T., Tanaka, M.
Organizer
Society for the Study of Reproduction 52nd Annual Meeting
Related Report
Int'l Joint Research
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