Biological roles of Ci-LF8, a novel Ciona-specific neuropeptide, on the regulation of ovarian follicle development

• Project/Area Number: 19K06752
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44040:Morphology and anatomical structure-related
• Research Institution: Suntory Foundation for Life Sciences
• Principal Investigator: Honoo Satake 公益財団法人サントリー生命科学財団, 生物有機科学研究所・統合生体分子機能研究部, 主幹研究員 (20280688)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ペプチド / GPCR / カタユウレイボヤ / 卵胞 / 成長 / 神経ペプチド / 受容体 / 成熟 / 進化

Outline of Research at the Start

ホヤ特異的な新規神経ペプチドの一つ、Ci-LF8とその受容体を同定し、Ci-LF8が卵巣に特異的に発現していることを明らかにした。本研究では、Ci-LF8が制御する卵胞成長成熟機構を体系的に解明し、ホヤの卵胞成熟機構をさらに明らかにするとともに、脊索動物門における同機構の進化や多様化を明らかにする分子基盤を構築する。

Outline of Final Research Achievements

Previously, we identified a novel Ciona-specific neuropeptide, CiLF-8, and its cognate receptor, CiLF8-R. However, its biological role remains to be elucidated. In this study, we revealed that CiLF8-R is localized to pre-vitellogenic oocytes. Moreover, RNA-seq for CiLF8-treated and untreated oocytes demonstrated that CiLF8 regulated a variety of non-coding RNAs. These results suggest that CiLF8 is involved in the growth of pre-vitellogenic oocytes.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、カタユウレイボヤの種特異的なペプチドであるCi-LF8の卵胞における生理機能を明らかにすることで、これまで不明だったカタユウレイボヤの卵胞の成長成熟機構の一端を解き明かすものである。脊索動物門における卵胞の成長制御機構の共通性と多様性、すなわち、進化の過程をを分子レベルで解明していく突破口となる。今後は、他のペプチドの作用と統合することで、ホヤの卵胞成長成熟機構の全容の解明を明らかにしていく。

Research Products

• [Journal Article] Omics Studies for the Identification of Ascidian Peptides, Cognate Receptors, and Their Relevant Roles in Ovarian Follicular Development (2022)
  • Author(s): Kawada Tsuyoshi、Osugi Tomohiro、Matsubara Shin、Sakai Tsubasa、Shiraishi Akira、Yamamoto Tatsuya、Satake Honoo
  • Journal Title: Frontiers in Endocrinology Volume: 13 Pages: 858885-858885
  • DOI: 10.3389/fendo.2022.858885
  • Peer Reviewed / Open Access
• [Journal Article] d-Serine controls epidermal vesicle release via NMDA receptor, allowing tissue migration during the metamorphosis of the chordate <i>Ciona</i> (2022)
  • Author(s): Krasovec Gabriel、Hozumi Akiko、Yoshida Tomoyuki、Obita Takayuki、Hamada Mayuko、Shiraishi Akira、Satake Honoo、Horie Takeo、Mori Hisashi、Sasakura Yasunori
  • Journal Title: Science Advances Volume: 8 Issue: 10 Pages: 1-22
  • DOI: 10.1126/sciadv.abn3264
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative analysis of transcriptomic profiles among ascidians, zebrafish, and mice: Insights from tissue-specific gene expression (2021)
  • Author(s): Matsubara Shin、Osugi Tomohiro、Shiraishi Akira、Wada Azumi、Satake Honoo
  • Journal Title: PLOS ONE Volume: 16 Issue: 9 Pages: e0254308-e0254308
  • DOI: 10.1371/journal.pone.0254308
  • Peer Reviewed / Open Access
• [Journal Article] Identification and function of ETH receptor networks in the silkworm Bombyx mori (2021)
  • Author(s): Daubnerov Ivana、Roller Ladislav、Satake Honoo、Zhang Chen、Kim Young-Joon、?it?an Du?an
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 11693-11693
  • DOI: 10.1038/s41598-021-91022-8
  • Peer Reviewed / Open Access
• [Journal Article] Cionin, a vertebrate cholecystokinin/gastrin homolog, induces ovulation in the ascidian Ciona intestinalis type A (2021)
  • Author(s): Osugi Tomohiro、Miyasaka Natsuko、Shiraishi Akira、Matsubara Shin、Satake Honoo
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 10911-10911
  • DOI: 10.1038/s41598-021-90295-3
  • Peer Reviewed / Open Access
• [Journal Article] Vasopressin Promoter Transgenic and Vasopressin Gene-Edited Ascidian, Ciona intestinalis Type A (Ciona robusta): Innervation, Gene Expression Profiles, and Phenotypes (2021)
  • Author(s): Kawada Tsuyoshi、Shiraishi Akira、Matsubara Shin、Hozumi Akiko、Horie Takeo、Sasakura Yasunori、Satake Honoo
  • Journal Title: Frontiers in Endocrinology Volume: 12 Pages: 668564-668564
  • DOI: 10.3389/fendo.2021.668564
  • Peer Reviewed / Open Access
• [Journal Article] Functional characterization and related evolutionary implications of invertebrate gonadotropin-releasing hormone/corazonin in a well-established model species (2021)
  • Author(s): Fodor Istvan、Svigruha Rika、Bozs? Zsolt、T?th G?bor K.、Osugi Tomohiro、Yamamoto Tatsuya、Satake Honoo、Pirger Zsolt
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 10028-10028
  • DOI: 10.1038/s41598-021-89614-5
  • Peer Reviewed / Open Access
• [Journal Article] A Testis-Specific Long Noncoding RNA, Start, Is a Regulator of Steroidogenesis in Mouse Leydig Cells (2021)
  • Author(s): Otsuka Kai、Matsubara Shin、Shiraishi Akira、Takei Natsumi、Satoh Yui、Terao Miho、Takada Shuji、Kotani Tomoya、Satake Honoo、Kimura Atsushi P.
  • Journal Title: Frontiers in Endocrinology Volume: 12 Pages: 665874-665874
  • DOI: 10.3389/fendo.2021.665874
  • Peer Reviewed / Open Access
• [Journal Article] A dual enhancer-silencer element, DES-K16, in mouse spermatocyte-derived GC-2spd(ts) cells (2021)
  • Author(s): Bandara Thusitha A.M.K.、Otsuka Kai、Matsubara Shin、Shiraishi Akira、Satake Honoo、Kimura Atsushi P.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 534 Pages: 1007-1012
  • DOI: 10.1016/j.bbrc.2020.10.049
  • NAID: 120007181810
  • Peer Reviewed
• [Journal Article] Distribution and physiological effect of enterin neuropeptides in the olfactory centers of the terrestrial slug Limax. (2020)
  • Author(s): *Matsuo R, Kobayashi S, Furuta A, Osugi T, Takahashi T, Satake H, and Matsuo Y
  • Journal Title: J Comp Physiol A Volume: in press Issue: 3 Pages: 401-418
  • DOI: 10.1007/s00359-020-01400-2
  • Peer Reviewed
• [Journal Article] The ventral peptidergic system of the adult ascidian Ciona robusta (Ciona intestinalis Type A) insights from a transgenic animal model (2020)
  • Author(s): Osugi Tomohiro、Sasakura Yasunori、Satake Honoo
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 1892-1892
  • DOI: 10.1038/s41598-020-58884-w
  • NAID: 120007163333
  • Peer Reviewed / Open Access
• [Journal Article] GABA-Induced GnRH Release Triggers Chordate Metamorphosis. (2020)
  • Author(s): Hozumi A, Matsunobu S, Mita K, Treen N, Sugihara T, Horie T, Sakuma T, Yamamoto T, Shiraishi A, Hamada M, Satoh N, Sakurai K, Satake H, Sasakura Y.
  • Journal Title: Current Biology Volume: 30 Issue: 8 Pages: 1555-1561
  • DOI: 10.1016/j.cub.2020.02.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Invertebrate Gonadotropin-Releasing Hormone Receptor Signaling and Its Relevant Biological Actions (2020)
  • Author(s): Sakai Tsubasa、Yamamoto Tatsuya、Matsubara Shin、Kawada Tsuyoshi、Satake Honoo
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 22 Pages: 8544-8544
  • DOI: 10.3390/ijms21228544
  • Peer Reviewed / Open Access
• [Journal Article] A novel G protein-coupled receptor for starfish gonadotropic hormone, relaxin-like gonad-stimulating peptide (2020)
  • Author(s): Mita Masatoshi、Matsubara Shin、Osugi Tomohiro、Shiraishi Akira、Wada Azumi、Satake Honoo
  • Journal Title: PLOS ONE Volume: 15 Issue: 11 Pages: 0242877-0242877
  • DOI: 10.1371/journal.pone.0242877
  • Peer Reviewed / Open Access
• [Journal Article] A relaxin‐like gonad‐stimulating peptide identified from the starfish Astropecten scoparius (2020)
  • Author(s): Mita Masatoshi、Osugi Tomohiro、Matsubara Shin、Kawada Tsuyoshi、Satake Honoo、Katayama Hidekazu
  • Journal Title: Molecular Reproduction and Development Volume: 88 Issue: 1 Pages: 34-42
  • DOI: 10.1002/mrd.23444
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fractionation of Ovarian Follicles and in vitro Oocyte Maturation and Ovulation Assay of Ciona intestinalis Type A (2020)
  • Author(s): Matsubara Shin、Shiraishi Akira、Osugi Tomohiro、Kawada Tsuyoshi、Satake Honoo
  • Journal Title: BIO-PROTOCOL Volume: 10 Issue: 7
  • DOI: 10.21769/bioprotoc.3577
  • Peer Reviewed / Open Access
• [Journal Article] Mechanism of gamete shedding in starfish: Involvement of acetylcholine in extracellular Ca2+-dependent contraction of gonadal walls (2020)
  • Author(s): Mita Masatoshi、Osugi Tomohiro、Takahashi Toshio、Watanabe Takehiro、Satake Honoo
  • Journal Title: General and Comparative Endocrinology Volume: 290 Pages: 113401-113401
  • DOI: 10.1016/j.ygcen.2020.113401
  • Peer Reviewed
• [Journal Article] Repertoires of G protein-coupled receptors for Ciona-specific neuropeptides (2019)
  • Author(s): Akira Shiraishi, Toshimi Okuda, Natsuko Miyasaka, Tomohiro Osugi, Yasushi Okuno, Jun Inoue, and Honoo Satake
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 116 (16) Issue: 16 Pages: 7847-7856
  • DOI: 10.1073/pnas.1816640116
  • Peer Reviewed / Open Access
• [Journal Article] Functional characterization of an invertebrate gonadotropin-releasing hormone receptor in the Yesso scallop Mizuhopecten yessoensis (2019)
  • Author(s): Nagasawa Kazue、Matsubara Shin、Satake Honoo、Osada Makoto
  • Journal Title: General and Comparative Endocrinology Volume: 282 Pages: 113201-113201
  • DOI: 10.1016/j.ygcen.2019.06.005
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a binding protein for sesamin and characterization of its roles in plant growth (2019)
  • Author(s): Tera Masayuki、Koyama Tomotsugu、Murata Jun、Furukawa Ayako、Mori Shoko、Azuma Toshiaki、Watanabe Takehiro、Hori Katsuhito、Okazawa Atsushi、Kabe Yasuaki、Suematsu Makoto、Satake Honoo、Ono Eiichiro、Horikawa Manabu
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 8631-8631
  • DOI: 10.1038/s41598-019-45003-7
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a New Theca/Interstitial Cell-Specific Gene and Its Biological Role in Growth of Mouse Ovarian Follicles at the Gonadotropin-Independent Stage (2019)
  • Author(s): Aoyama Masato、Shiraishi Akira、Matsubara Shin、Horie Kaoru、Osugi Tomohiro、Kawada Tsuyoshi、Yasuda Keiko、Satake Honoo
  • Journal Title: Frontiers in Endocrinology Volume: 10 Pages: 553-553
  • DOI: 10.3389/fendo.2019.00553
  • Peer Reviewed / Open Access
• [Journal Article] The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates (2019)
  • Author(s): Matsubara Shin、Shiraishi Akira、Osugi Tomohiro、Kawada Tsuyoshi、Satake Honoo
  • Journal Title: eLife Volume: 8
  • DOI: 10.7554/elife.49062
  • Peer Reviewed / Open Access
• [Journal Article] Editorial: Endocrine and Neuroendocrine Systems of Invertebrate Deuterostomes (2019)
  • Author(s): Satake Honoo、Larhammar Dan、Elphick Maurice R.
  • Journal Title: Frontiers in Endocrinology Volume: 10 Pages: 755-755
  • DOI: 10.3389/fendo.2019.00755
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Draft genome of Tanacetum cinerariifolium, the natural source of mosquito coil (2019)
  • Author(s): Yamashiro Takanori、Shiraishi Akira、Satake Honoo、Nakayama Koji
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 18249-18249
  • DOI: 10.1038/s41598-019-54815-6
  • Peer Reviewed / Open Access
• [Journal Article] Tachykinins: Neuropeptides That Are Ancient, Diverse, Widespread and Functionally Pleiotropic (2019)
  • Author(s): Nassel Dick R.、Zandawala Meet、Kawada Tsuyoshi、Satake Honoo
  • Journal Title: Frontiers in Neuroscience Volume: 13 Pages: 1262-1262
  • DOI: 10.3389/fnins.2019.01262
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 機械学習＋実験のコンビネーションで見えた、 新たなペプチド―受容体相互作用の姿 (2021)
  • Author(s): 佐竹炎
  • Organizer: 第45回日本比較内分泌学会金沢大会
  • Invited