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Studies on the regulation of the gonadal regression of seasonal breeding using intraspecific variation in the teleost fish medaka, Oryzias latipes

Research Project

Project/Area Number 19K06785
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45010:Genetics-related
Research InstitutionNational Institute for Basic Biology (2022-2023)
Center for Novel Science Initatives, National Institutes of Natural Sciences (2019-2021)

Principal Investigator

Shinomiya Ai  基礎生物学研究所, バイオリソース研究室, 助教 (60452067)

Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords光周性 / 季節繁殖 / QTL解析 / 種内変異 / メダカ / 地理的変異 / 次世代シーケンス / 生殖腺指数 / QTL-seq / RNA-seq / 卵巣
Outline of Research at the Start

メダカは日長の季節変化に対して明瞭な応答を示す。日本各地に由来する系統について短日暴露による繁殖停止の誘導を解析した結果、低緯度の系統において短日応答性が喪失していることが明らかとなった。本研究では、短日応答の種内変異を利用して、次世代シーケンサーを利用した遺伝学的解析およびトランスクリプトーム解析、遺伝子編集技術を応用した機能解析を行い、秋(短日)の感知に関わる遺伝子、繁殖停止を制御する遺伝子を明らかにすることを目的とする。本研究により脊椎動物の日長感知と繁殖停止の分子メカニズムの理解を目指す。

Outline of Final Research Achievements

Although the teleost medaka, Oryzias latipes, is known to be long-day breeder, we had previously shown that wild populations originating from low latitudes had genetically lower sensitivity to photoperiod. In this study, we used three wild populations including the short-day "responsive" Kiyosu and Kushima and "non-responsive" Miyazaki. Phenotypes of the F2 individuals in two types of crosses were examined, and three quantitative trait loci (QTLs) were identified on chromosomes 9, 6 and 20 by QTL analyses using whole genome sequences of the F2 generations. We compared the sequences of the QTL regions between "responsive" and "non-responsive" strains and performed transcriptome analyses (RNA-Seq) of Kiyosu and Miyazaki under long-day and short-day conditions, and discussed the genes involved in response to short-day and gonadal regression.

Academic Significance and Societal Importance of the Research Achievements

動植物の成長や繁殖に深く関わる季節適応の仕組みの解明は、基礎生物学的観点、また農水産業への応用の面からも重要である。多くの季節繁殖動物で、日長変化が繁殖期の引き金となることが知られているが、日長の感知とその下流で働く分子機構はまだ不明な点が多い。本研究で、メダカの日長変化への応答性の種内変異を利用して同定した、短日に応じた繁殖停止に関わる量的形質遺伝子座(QTLs)、短日応答時の遺伝子発現動態の変化は、季節繁殖停止の分子メカニズムの理解につながることが期待される。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (4 results)

All 2023 2022 2020

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (1 results) Book (1 results)

  • [Journal Article] Variation in responses to photoperiods and temperatures in Japanese medaka from different latitudes2023

    • Author(s)
      Shinomiya A, Adachi D, Shimmura T, Tanikawa M, Hiramatsu N, Shigeho I, Naruse K, Sakaizumi M, Yoshimura T
    • Journal Title

      Zoological Letters

      Volume: 9 Issue: 1 Pages: 16-16

    • DOI

      10.1186/s40851-023-00215-8

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic analysis of body weight in wild populations of medaka fish from different latitudes2020

    • Author(s)
      (1) Yassumoto TI, Nakatsukasa M, Nagano AJ, Yasugi M, Yoshimura T, Shinomiya A
    • Journal Title

      PLoS One

      Volume: e0234803 Issue: 6 Pages: e0234803-e0234803

    • DOI

      10.1371/journal.pone.0234803

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 野生メダカを利用した短日による季節繁殖停止機構の遺伝学的解析2022

    • Author(s)
      四宮愛、Imaeda Yassumoto Tamiris、吉村崇
    • Organizer
      日本動物学会 第93回 早稲田大会
    • Related Report
      2022 Research-status Report
  • [Book] 「動物の体色がわかる図鑑 動物たちの色はこうして決まる」(担当「特集 動物が見る世界の彩」)2022

    • Author(s)
      池田譲、伊藤祥輔、合田真、近藤滋、四宮愛、高橋明義、橋本寿史、廣部知久、福澤利彦、二橋美瑞子、二橋亮、持田浩治、森本元、吉岡伸也、若松一雅 著/秋山豊子 監修
    • Total Pages
      322
    • Publisher
      グラフィック社
    • ISBN
      9784766136272
    • Related Report
      2022 Research-status Report

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Published: 2019-04-18   Modified: 2025-01-30  

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