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Sex determination in multiple-sex chromosome system including three major sex-determining genes and its evolutionary mechanism

Research Project

Project/Area Number 19K06788
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45020:Evolutionary biology-related
Research InstitutionHiroshima University

Principal Investigator

Miura Ikuo  広島大学, 両生類研究センター, 准教授 (10173973)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords性染色体 / 性決定 / XY / ZW / ターンオーバー / カエル / 複合型性染色体 / 転座 / オスヘテロ
Outline of Research at the Start

脊椎動物では、これまで少なくとも3種類の主要な性決定遺伝子が同定されている。私たちは、染色体の相互転座によってこれら3つの遺伝子をつなぐ複合型性染色体をカエルに見出した。さらに、同種内で未だ相互転座が生じていない元祖集団も発見した。本研究では、3つの性決定遺伝子で構成される複合型性染色体の性決定様式と元祖型を起点としたその進化機構の解明に臨む。

Outline of Final Research Achievements

We identified a multiple sex chromosome system which was created by translocations among male three chromosomes in Taiwanese frog Odorrana swinhoana. And, in the closely related species Odorrana utsunomiyaorum inhabiting Japan (Iriomote and Ishigaki Islands), we identified turnover of sex chromosomes, transition of heterogametic sex from XY to ZW, and evolution from homomorphy to heteromorphy. This is a highly rapid and dynamic sex chromosome evolution that has never been found in vertebrates.

Academic Significance and Societal Importance of the Research Achievements

性、すなわち有性生殖は、生物個体の遺伝的多様性を生み出し、生物の進化に貢献する重要な生殖様式である。また、人類を含む真核生物の起源において誕生した独特な仕組みの一つである。性やジェンダーに関する問題は近年社会問題化しており、現代の人間社会において重要な課題である。本研究は、ヒトや哺乳類の研究では知ることができない性の多様性やその進化機構に関する疑問を解決するためのヒントを与えてくれる。この意味では重要な生物学的基礎研究である。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (36 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (5 results) Journal Article (9 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (18 results) (of which Int'l Joint Research: 8 results,  Invited: 6 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] 台湾国立師範大学(台湾)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of Canberra(オーストラリア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 台湾師範大学(台湾)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] 台湾国立師範大学(台湾)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] カセサート大学(タイ)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Phylogenetic Origins of a Newly Found Japanese Red-bellied Newt Population in Yokohama City and Other Populations in Kanagawa Prefecture2022

    • Author(s)
      Senzaki M, Sano S, Takemoto A, Miura I, Ogata M
    • Journal Title

      Current Herpetology

      Volume: 41 Issue: 1 Pages: 132-137

    • DOI

      10.5358/hsj.41.132

    • NAID

      130008164509

    • ISSN
      1345-5834, 1881-1019
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly rapid and diverse sex chromosome evolution in the frog Odorrana species complex2022

    • Author(s)
      Katsumi T, FShams F, Yanagi H, Ohnishi T, Toda M, Lin S-M, Mawaribuchi S, Shimizu N, Ezaz T, Miura I
    • Journal Title

      Development growth & differentiation

      Volume: -

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Sex-chromosome evolution from a heteromorphic to a homomorphic system by inter-population hybridization in a frog2021

    • Author(s)
      Ogata M, Suzuki K, Yuasa Y, and Miura I
    • Journal Title

      Philos. Trans. R. Soc. B

      Volume: 376 Issue: 1833 Pages: 20200105-20200105

    • DOI

      10.1098/rstb.2020.0105

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Evolution of a multiple sex-chromosome system by three-sequential translocations among potential sex-chromosomes in the Taiwanese frog Odorrana swinhoana.2021

    • Author(s)
      Miura I, Shams F, Lin S-M, 1, Cioffi MB, Liehr T, Al‐Rikabi A, Kuwana C, Srikulnath K, Higaki Y, Ezaz E.
    • Journal Title

      Cells

      Volume: 10 Issue: 3 Pages: 661-661

    • DOI

      10.3390/cells10030661

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Hybridogenesis in the Water Frogs from Western Russian Territory: Intrapopulation Variation in Genome Elimination.2021

    • Author(s)
      Miura I, Vershinin V, Vershinina S, Lebedinsky A, Trofimov A, Sitnikov I and Ito M
    • Journal Title

      Genes

      Volume: 12 Issue: 2 Pages: 244-244

    • DOI

      10.3390/genes12020244

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative genomics of Glandirana rugosa using unsupervised AI reveals a high CG frequency2021

    • Author(s)
      Katsura Y, Ikemura T, Kajitani R, Toyoda A, Itoh T, Ogata M, Miura I, Wada K, Wada Y, and Satta Y
    • Journal Title

      Life Science Alliance

      Volume: - Issue: 5 Pages: e202000905-e202000905

    • DOI

      10.26508/lsa.202000905

    • NAID

      120007153083

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolution of sex-chromosome heteromorphy in geographic populations of the Japanese Tago’s brown frog complex.2021

    • Author(s)
      Kuwana C, Fujita H, Tagami M, Matsuo T, and Miura I
    • Journal Title

      Cytogenetic and Genome Research

      Volume: -

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chromosomics: Bridging the Gap between Genomes and Chromosomes2019

    • Author(s)
      Deakin JE, Potter S, O'Neill R, Ruiz-Herrera A, Cioffi MB, Eldridge MDB, Fukui K, Marshall Graves JA, Griffin D, Grutzner F, Kratochv, L, Miura I, Rovatsos M, Srikulnath K, Wapstra E, Ezaz T
    • Journal Title

      Genes

      Volume: 10 Issue: 8 Pages: 627-627

    • DOI

      10.3390/genes10080627

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mitochondrial heteroplasmy in marsh frog (Pelophylax ridibundus Pallas, 17772019

    • Author(s)
      Vershinin V, Sitnikov I, Vershinina S, Trofimov A, Lebedinsky A and Miura I
    • Journal Title

      Russian Journal of Genetics

      Volume: 55 Pages: 1041-1045

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 6本の性染色体をもつカエルの祖先型性染色体の同定と近縁種間で生じたターンオーバーの発見2021

    • Author(s)
      三浦郁夫、林思民、大西拓、戸田守、桂有加子、回渕修治、Foyez Sham、Tariq Ezaz
    • Organizer
      第73回一般財団法人染色体学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 異種交配における片親ゲノム特異的排除(雑種生成)の分子機構の解明に向けて:トランスポゾンvs piRNAs2021

    • Author(s)
      須田皓介, 三浦郁夫, 鈴木貴大、林舜,廣田直哉, 田村啓,高松信彦, Vershinin Vladimir, 伊藤道彦
    • Organizer
      分子生物学会第44回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ツチガエルにおけるX、Y、ZおよびW染色体連鎖遺伝子群の分子進化2021

    • Author(s)
      回渕修治、三浦郁夫
    • Organizer
      分子生物学会第44回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 性染色体の動的進化:ターンオーバーとリサイクル2021

    • Author(s)
      三浦郁夫、尾形光昭
    • Organizer
      第23回進化学会シンポジウム 「動物の性決定システムの進化」
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Evolutionary history of sex chromosomesin the frog Glandirana rugosa:Two times turnover and four times recycling2021

    • Author(s)
      Miura I and Ogata M
    • Organizer
      23rd International Chromosome Conference (ICC)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Frog and Biology: Sex, Evolution and DNA music2021

    • Author(s)
      Miura I
    • Organizer
      lessons learned from global partnerships (Kasetsart University, Thailand)
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Optimistic destiny of sex chromosome evolution in frogs2020

    • Author(s)
      Miura I and Ogata M
    • Organizer
      7th Asian-Pacific Chromosome Colloquium
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Sex chromosome evolution in Japanese tago's brown frog species complex2020

    • Author(s)
      Kuwana C, Fujita H, Tagami M, Matsuo T, Sriklunath K, Miura I
    • Organizer
      7th Asian-Pacific Chromosome Colloquium
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Evolutionary genetic study on giant salamander, the geographic populations newly found in Hiroshima Prefecture, Japan.2020

    • Author(s)
      Goto S, Shimizu N, Tsubota H, Ikeda S, Taguchi Y, Minami S and Miura I
    • Organizer
      7th Asian-Pacific Chromosome Colloquium
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Six sex chromosomes in a Taiwanese frog2020

    • Author(s)
      Miura I, Kuwana C, Lin SM, and Srikulnath K
    • Organizer
      9th world congress of herpetoloty
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Sex chromosome evolution in Japanese tago brown frog species complex2020

    • Author(s)
      Kuwana C, Hiroyuki Fujita H, Tagami M, Sriklunath K, and Miura I
    • Organizer
      9th world congress of herpetoloty
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 6本の性染色体をもつカエル2019

    • Author(s)
      三浦郁夫、桑名知碧、林思民、Srikulnath Kornsorn
    • Organizer
      日本進化学会
    • Related Report
      2019 Research-status Report
  • [Presentation] ロシアウラル地方におけるヨーロッパトノサマガエルのゲノム排除機構と集団構造2019

    • Author(s)
      三浦郁夫、Vershinin Vradimir, Vershinina Svetlana, Ledivenski Andrei
    • Organizer
      日本爬虫両棲類学会
    • Related Report
      2019 Research-status Report
  • [Presentation] タゴガエルとナガレタゴガエルの性染色体の進化に関する研究(Rana tagoi,Rana sakuraii)2019

    • Author(s)
      桑名知碧,藤田宏之,田上正隆, Sriklunath Kornsorn, 三浦 郁夫
    • Organizer
      一般財団法人染色体学会
    • Related Report
      2019 Research-status Report
  • [Presentation] タゴガエル種複合体における性染色体の進化2019

    • Author(s)
      桑名知碧,藤田宏之,田上正隆, Sriklunath Kornsorn, 三浦 郁夫
    • Organizer
      日本爬虫両棲類学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 広島県で発見されてオオサンショウウオの新しい地域集団に関する進化遺伝学的研究2019

    • Author(s)
      後藤理史、清水則雄、坪田博美、池田誠慈、田口勇輝、三浦郁夫
    • Organizer
      日本爬虫両棲類学会
    • Related Report
      2019 Research-status Report
  • [Presentation] Sex chromosome recycling and turnover in frogs2019

    • Author(s)
      Miura I, Ogata M, Lin S-M
    • Organizer
      The workshop: Paradigm shift in sex chromosome evolution
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] The nature of hybridogenesis in the edible frog Pelophylax esculentus from Ural district, Russia2019

    • Author(s)
      Miura I
    • Organizer
      International Russian Japanese symposium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 両生類に魅せられて "ツチガエルで起きている地域集団および種間のせめぎ合い"2019

    • Author(s)
      尾形光昭、三浦郁夫
    • Total Pages
      175
    • Publisher
      市民科学出版
    • ISBN
      9784991053504
    • Related Report
      2019 Research-status Report
  • [Remarks] 生物はなぜ、どのように進化していくのか -その分子機構に迫る-

    • URL

      https://amphibian.hiroshima-u.ac.jp/~miura/first.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 【研究成果】6本の性染色体をもつカエルを発見

    • URL

      https://www.hiroshima-u.ac.jp/news/63680

    • Related Report
      2020 Research-status Report
  • [Remarks] 生物はなぜ、どのように進化していくのか

    • URL

      https://home.hiroshima-u.ac.jp/amphibia/miura/first.html

    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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