| Project/Area Number |
19K06798
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 45020:Evolutionary biology-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
PASCUAL JUAN 国立研究開発法人理化学研究所, 開拓研究本部, 研究員 (30594098)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | hematopoiesis / vertebrates / evolution / evo-devo / Evo-Devo / lamprey |
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| Outline of Research at the Start |
Vertebrates consists of two major groups: jawed vertebrates (mammals, reptiles, fish, etc) and jawless vertebrates (lampreys and hagfishes). While much is known about the formation of blood cells in mammals and fish, how these are formed in more distant vertebrates - both jawless and jawed- is unkown.
My research line hence focuses on studying some of these group of vertebrates for which our knowledge about their hematopoiesis system is limited.
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| Outline of Annual Research Achievements |
Hematopoiesis is the process by which the blood cells of an individual, from the embryo to adulthood, are formed. However, despite being a crucial physiological phenomenon of
animals, most of our knowledge derives from studies using a handful of classic animal models, mostly representatives of jawed vertebrates. With
this in mind, I set to study the lamprey, long-distant relative of jawed vertebrates that is understudied in terms of the blood system. In FY2020 I completed the analysis of RNA-seq data from lamprey embryos and identified all candidate hematopoietic markers in the lamprey. I generated a complete panel of expression patterns for all putative hematopoietic markers during lamprey embryogenesis. I was then able to perform CRISPR knock-outs of lamprey Scl gene, an important hematopoietic factor in other vertebrates, and obtained enough samples to carry out RNA-seq analysis as well as in situ hybridization experiments. Although there was no apparent mutant phenotype, blood alterations are expected. These samples will be used in the near future to assess the function of Scl in blood development in cyclostomes, what will enable comnparative analysis with gnathostome hematopoiesis.
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