Reassessment of fungal species composition in Japan based on verification of universality and uniqueness

• Project/Area Number: 19K06802
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 45030:Biodiversity and systematics-related
• Research Institution: Hirosaki University
• Principal Investigator: Tanaka Kazuaki 弘前大学, 農学生命科学部, 教授 (60431433)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 菌類 / 種分類 / 隠蔽種 / 分類体系 / 生物多様性 / 固有種 / 系統 / 種

Outline of Research at the Start

日本の植物寄生菌には、欧米産の菌種と同一種であるとされているものが多い。一方、日本からしか見つからない独自の種とされているものも存在する。しかし、これらの知見は菌類の種同定に関する間違った「前提」に基づく誤解である可能性が高い。菌類種同定に関する従前の前提を再検討し、菌類の普遍性と固有性を検証することで、日本における菌類種構成の実体解明を試み、これまでの予想以上にその多様性が高いことを明らかにする。

Outline of Final Research Achievements

Plant parasitic fungi in Japan have been identified mainly based on two assumptions. For example, fungi on woody plants such as beeches (Fagus spp.) have been considered to be the same species (universal species) between Japan and Europe/America (Assumption 1). It has also been believed that some fungal species on bamboos in Japan are composed of unique species (endemic species) (Assumption 2). These assumptions about species boundaries of ascomycetous fungi in Japan were tested by morphological comparison of phenotypic characters and phylogenetic analyses using molecular data. Assumption 1 was generally rejected, while assumption 2 was generally accepted. One new order and ten new species (including eight new combinations) of ascomycetous fungi were discovered and described, demonstrating species diversity of fungi in Japan.

Academic Significance and Societal Importance of the Research Achievements

日本産菌類の種に関する「普遍性」と「固有性」という対照的な特徴について、形態学的・分子系統学的に検証した。結果として日本の菌類相は従来考えられていたよりも、より多様な種により構成されていることを明らかにした。菌類の種同定に関連した従前の前提を見直すことで、菌類における種の考え方を根本的に変えていくきっかけになることが期待できる。本研究課題を進めるなかで約400株以上の純粋培養株を新たに取得した。これらを微生物遺伝資源として系統保存するとともに、有用代謝産物の探査源として整備・利用した。

Research Products

• [Int'l Joint Research] Kunming University/Zhongkai University/Southwest University(中国)
• [Int'l Joint Research] Chiang Mai University/Mae Fah Luang University/Mashroom Research Foundation(タイ)
• [Int'l Joint Research] University of North Carolina/University of Illinois(米国)
• [Int'l Joint Research] Manaaki Whenua-Landcare Research(ニュージーランド)
• [Int'l Joint Research] University of British Columbia(カナダ)
• [Int'l Joint Research]
• [Int'l Joint Research] Qujing Normal University/Chinese Academy of Sciences/Kunming Institute of Botany(中国)
• [Int'l Joint Research] UFMS/UFRN/UFPB(ブラジル)
• [Int'l Joint Research] Freie University Berlin/University of Bayreuth/SNSB(ドイツ)
• [Int'l Joint Research] Purdue University/The University of Alabama/University of Arkansas(米国)
• [Int'l Joint Research] National Fungal Culture Collection/Agharkar Research Institute/Birbal Sahni Institute of Palaeosciences(インド)
• [Int'l Joint Research]
• [Journal Article] Thyridium revised: Synonymisation of Phialemoniopsis under Thyridium and establishment of a new order, Thyridiales (2022)
  • Author(s): Sugita R, Tanaka K
  • Journal Title: MycoKeys Volume: 86 Pages: 147-176
  • DOI: 10.3897/mycokeys.86.78989
  • Peer Reviewed / Open Access
• [Journal Article] Decaspirones and palmarumycins from Phaeoseptum sp. KT4106: Chirality reinvestigation of palmarumycins CP4a and CP5 (2021)
  • Author(s): Kanehara R, Hayasaka A, Inose K, Okamura T, Miura S, Tanaka K, Hashimoto M
  • Journal Title: Tetrahedron Volume: 92 Pages: 132251-132251
  • DOI: 10.1016/j.tet.2021.132251
  • Peer Reviewed / Open Access
• [Journal Article] Isolation and structural elucidation of novel fusicoccan dehydroxypericonicin A from Roussoella sp. (2021)
  • Author(s): Hayasaka A, Tanaka K, Hashimoto M
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 85 Issue: 8 Pages: 1798-1801
  • DOI: 10.1093/bbb/zbab103
  • Peer Reviewed / Open Access
• [Journal Article] Cyclohelminthol CPs: Scope and Limitations of Density Functional Theory-Based Structural Elucidation of Natural Products (2021)
  • Author(s): Inose K, Tanaka S, Tanaka K, Hashimoto M
  • Journal Title: The Journal of Organic Chemistry Volume: 86 Issue: 2 Pages: 1505-1515
  • DOI: 10.1021/acs.joc.0c02378
  • Peer Reviewed
• [Journal Article] DFT Supported Structural Revision of Seiricardines (2020)
  • Author(s): Nishiyama M, Tayone WC, Maeda H, Tanaka K, Hashimoto M
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 93 Issue: 6 Pages: 768-775
  • DOI: 10.1246/bcsj.20200069
  • NAID: 130007856020
  • Peer Reviewed
• [Journal Article] DFT supported structural elucidations of seiridiasteriscane A, unique 15-norasteriscane and novel pestalotiopsin Congeners from Seiridium sp. KT3957 (2020)
  • Author(s): Wilanfranco C. Tayone, Mami Nishiyama, Kazuaki Tanaka, Maeda Hayato, Masaru Enomoto, Masaru Hashimoto
  • Journal Title: Tetrahedron Letters Volume: 76 Issue: 20 Pages: 131197-131197
  • DOI: 10.1016/j.tet.2020.131197
  • Peer Reviewed
• [Journal Article] Taxonomical re-examination of the genus Phyllosticta; Parasitic fungi on Cupressaceae trees in Japan (2020)
  • Author(s): Hattori Y, Motohashi K, Tanaka K, Nakashima C
  • Journal Title: Forest Pathology Volume: 50
  • Peer Reviewed
• [Journal Article] Freshwater Dothideomycetes (2020)
  • Author(s): Dong W, Wang B, Hyde KD, McKenzie EHC, Raja HA, Tanaka K, Abdel-Wahab MA, Abdel-Aziz FA, Doilom MA, Phookamsak R, Hongsanan S, Wanasinghe DN, Yu XD, Wang GN, Yang HA, Yang J, Thambugala KM, Tian Q, Luo ZL, Yang JB, Miller AN, Fournier J, Boonmee S, Hu DM, Nalumpang S, Zhang H
  • Journal Title: Fungal Diversity Volume: 105 Issue: 1 Pages: 319-575
  • DOI: 10.1007/s13225-020-00463-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Outline of Fungi and fungus-like taxa (2020)
  • Author(s): Wijayawardene NN, Hyde KD, Al-Ani LKT, Tedersoo L, Haelewaters D, Rajeshkumar KC et al. (Tanaka K, 96番目/158名中)
  • Journal Title: Mycosphere Volume: 11 Issue: 1 Pages: 1060-1456
  • DOI: 10.5943/mycosphere/11/1/8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cyclopericodiol and new chlorinated melleins isolated from Periconia macrospinosa KT3863 (2019)
  • Author(s): Inose K, Tanaka K, Koshino H, Hashimoto M
  • Journal Title: Tetrahedron Volume: 75 Issue: 35 Pages: 130470-130470
  • DOI: 10.1016/j.tet.2019.130470
  • Peer Reviewed
• [Presentation] Thyridium 属に関する分類学的研究 (2020)
  • Author(s): 杉田綾祐・田中和明
  • Organizer: 日本菌学会第64回大会
• [Presentation] 日本産 Hysterium 目菌の分類学的検討 (2020)
  • Author(s): 田中雅也・橋本陽・田中和明
  • Organizer: 日本菌学会第64回大会
• [Presentation] 青森県で採集された Pezicula 属菌2種に関する分類学的検討 (2020)
  • Author(s): 芳賀志保・大木保善・田中和明
  • Organizer: 日本菌学会第64回大会