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A study of diversity of minute brown algae using culture and molecular analyses

Research Project

Project/Area Number 19K06817
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionHokkaido University

Principal Investigator

Kogame Kazuhiro  北海道大学, 理学研究院, 教授 (80215219)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords褐藻 / 微小褐藻 / DNAバーコーディング / cox1 / COI / 分子系統 / 培養 / 分子系統解析 / 多様性 / 分類
Outline of Research at the Start

褐藻類には,大形の藻体をもつものばかりではなく,微視的な種も存在する。巨視的な種が形態的にも分子系統学的にも研究が進められているのに比較して,微視的な種は近年の分子系統学的研究の進展から取り残されており,その多様性に関する我々の知識は大変乏しい。本研究では,微小褐藻に特に注目して,採集,培養株の確立,形態観察,分子系統解析を行い,微小褐藻の多様性を明らかにしていく。日本各地から採集サンプルを得て,多くの培養株を確立し,シトクローム酸化酵素サブユニット1遺伝子を用いて系統解析を行う。それにより,微小褐藻の種多様性を明らかにし,地理的分布を把握するとともに,種の記載,分類体系の検討を進める。

Outline of Final Research Achievements

Brown algae include not only large species such as kelps, but also small species less than a few millimeters in size. The taxonomy of such minute brown algae is confusing because of the lack of clear taxonomic characters, making species identification difficult. In this study, isolates of minute brown algae were established from samples collected from the Japanese coasts, and DNA sequences were determined for the isolates in order to use the DNA sequences for understanding of the species classification and diversity of minute brown algae. DNA sequences were determined for 245 of 368 isolates. 24 isolates were identified at species level, and 5 species were considered to be new to Japan.

Academic Significance and Societal Importance of the Research Achievements

褐藻類にはコンブのような大形の種類だけではなく,数mm以下の小さなものもいる。そのような微小褐藻は,明瞭な分類形質が少なく分類が混乱しており,種同定が難しい。そこで,DNA塩基配列決定し,DNA塩基配列を用いて,微小褐藻の種分類,多様性の理解を目指した。微小褐藻の種をDNAで区別できるようになることで,分類だけでなく,これまで行うことができなかった微小褐藻の生態学的研究や他の生物との関係,環境評価,有用成分の探索など,他分野の研究の発展につながることが期待される。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (6 results)

All 2023 2022 2021 2020

All Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] Colpomenia borea sp. nov. (Scytosiphonaceae, Phaeophyceae) from Japan and Russian Far East2023

    • Author(s)
      Michael Jacob C. Dy, Masakazu Hoshino, Tsuyoshi Abe, Norishige Yotsukura, Nina Klochkova, Kyung Min Lee, Sung Min Boo and Kazuhiro Kogame
    • Journal Title

      Phycological Research

      Volume: 71 Issue: 2 Pages: 81-89

    • DOI

      10.1111/pre.12510

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Asterocladon ednae sp. nov. (Asterocladales, Phaeophyceae) from the Philippines2022

    • Author(s)
      Eriko Sasagawa, Wilfred John E. Santianez, Kazuhiro Kogame
    • Journal Title

      Phycological Research

      Volume: 70 Issue: 4 Pages: 185-191

    • DOI

      10.1111/pre.12490

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Geographical parthenogenesis in the brown alga Scytosiphon lomentaria (Scytosiphonaceae): Sexuals in warm waters and parthenogens in cold waters2021

    • Author(s)
      Hoshino Masakazu, Hiruta Shimpei F., Croce Maria Emilia, Kamiya Mitsunobu, Jomori Takahiro, Wakimoto Toshiyuki & Kogame Kazuhiro
    • Journal Title

      Molecular Ecology

      Volume: 30 Issue: 22 Pages: 5814-5830

    • DOI

      10.1111/mec.16152

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Systematics, distribution, and sexual compatibility of six Scytosiphon species (Scytosiphonaceae, Phaeophyceae) from Japan and the description of four new species2021

    • Author(s)
      Masakazu Hoshino, Atsuko Tanaka, Mitsunobu Kamiya, Shinya Uwai, Masanori Hiraoka, Kazuhiro Kogame
    • Journal Title

      Journal of Phycology

      Volume: 57 Pages: 416-434

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 褐藻類の微小藻体のCOIバーコーディング2022

    • Author(s)
      桝山海里,関本瑠菜,小亀一弘
    • Organizer
      日本藻類学会第46回大会(オンライン福井大会)
    • Related Report
      2021 Research-status Report
  • [Presentation] COIバーコーディングによる微小褐藻の多様性2020

    • Author(s)
      関本瑠菜,小亀一弘
    • Organizer
      日本藻類学会第44回大会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2024-01-30  

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