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Mechanisms of spontaneous activity-dependent synaptic competition in dendrite remodeling

Research Project

Project/Area Number 19K06886
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 46010:Neuroscience-general-related
Research InstitutionKyushu University

Principal Investigator

Fujimoto Satoshi  九州大学, 医学研究院, 助教 (50586592)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords神経回路形成 / 樹状突起 / 自発神経活動 / 嗅球 / シナプス刈り込み / Rho GTPases / Rho GTPase / 光遺伝学
Outline of Research at the Start

神経細胞が機能的な脳を構成するためには、シナプスの刈り込みを介した回路の精緻化が必要であり、その過程には神経活動の関与が示唆されている。本研究では、発達期の嗅覚僧帽細胞をモデルとして、樹状突起の選択的刈り込みを介した「勝ち」「負け」の決定が自発神経活動のタイミングや強度など、いかなるパラメーターよって制御されるのかについて明らかにする。

Outline of Final Research Achievements

During development, neuronal activity plays an essential role in dendritic remodeling. However, the mechanism stabilizing synapses that receive a strong input and prunes the remaining synapses is poorly understood. A mechanism that determines "winners" and "losers" through synaptic competition has been postulated, but the mechanism is unknown. In this study, using mitral cells as a model, we show that an NMDA receptor-RhoA signaling-dependent local protection and lateral inhibition control synaptic competition and a general principle of neuronal receptive field formation.

Academic Significance and Societal Importance of the Research Achievements

正しい回路が「作られる」仕組みは多く研究されてきたが、不要な回路の「刈り込み」の原理は長年の謎であった。発達期のシナプス再編成は脳のいたるところで起こっており、本研究で明らかとなったRhoAを介したシナプス競合と神経突起の刈り込み機構は、神経回路再編成における普遍的な原理であると考えられる。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (18 results)

All 2023 2022 2021 2020 2019

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Open Access: 5 results,  Peer Reviewed: 2 results) Presentation (12 results) (of which Int'l Joint Research: 8 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Automated neuronal reconstruction with super-multicolour fluorescence imaging2022

    • Author(s)
      Leiwe Marcus N.、Fujimoto Satoshi、Baba Toshikazu、Moriyasu Daichi、Saha Biswanath、Sakaguchi Richi、Inagaki Shigenori、Imai Takeshi
    • Journal Title

      bioRxiv

      Volume: - Pages: 512984-512984

    • DOI

      10.1101/2022.10.20.512984

    • Related Report
      2022 Annual Research Report
    • Open Access
  • [Journal Article] BMPR-2 gates activity-dependent stabilization of primary dendrites during mitral cell remodeling2021

    • Author(s)
      Shuhei Aihara, Satoshi Fujimoto, Richi Sakaguchi, Takeshi Imai
    • Journal Title

      Cell Reports

      Volume: 35 Issue: 12 Pages: 109276-109276

    • DOI

      10.1016/j.celrep.2021.109276

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Post hoc Correction of Chromatic Aberrations in Large-Scale Volumetric Images in Confocal Microscopy2021

    • Author(s)
      Leiwe Marcus N.、Fujimoto Satoshi、Imai Takeshi
    • Journal Title

      Frontiers in Neuroanatomy

      Volume: 15 Pages: 0-1

    • DOI

      10.3389/fnana.2021.760063

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] BMPR-2 gates activity-dependent stabilization of dendrites during mitral cell remodeling2020

    • Author(s)
      Shuhei Aihara, Satoshi Fujimoto, Richi Sakaguchi, Takeshi Imai
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2020.10.30.358861

    • Related Report
      2020 Research-status Report
    • Open Access
  • [Journal Article] Spontaneous activity generated within the olfactory bulb establishes the discrete wiring of mitral cell dendrites2019

    • Author(s)
      Satoshi Fujimoto, Marcus N. Leiwe, Richi Sakaguchi, Yuko Muroyama, Reiko Kobayakawa, Ko Kobayakawa, Tetsuichiro Saito, Takeshi Imai
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/625616

    • Related Report
      2019 Research-status Report
    • Open Access / Int'l Joint Research
  • [Presentation] Activity-dependent lateral inhibition signals for synaptic competition2023

    • Author(s)
      Satoshi Fujimoto
    • Organizer
      IRCN-iPlasticity International Symposium 2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activity-dependent lateral inhibition signals for synaptic competition2023

    • Author(s)
      Satoshi Fujimoto, Marcus Leiwe, Shuhei Aihara, Richi Sakaguchi, Yuko Muroyama, Reiko Kobayakawa, Ko Kobayakawa, Tetsuichiro Saito, Takeshi Imai
    • Organizer
      EMBO Workshop - Mechanisms of neuronal remodelling, 2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シナプス競合による樹状突起刈り込みのメカニズム2023

    • Author(s)
      藤本聡志
    • Organizer
      第5回これからの神経回路研究の会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Super-multicolour labelling of neurons for automated circuit reconstructions2022

    • Author(s)
      Marcus N. Leiwe, Satoshi Fujimoto, Toshikazu Baba, Takeshi Imai,
    • Organizer
      NEURO2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activity-dependent competition within a neuron ensures the discrete wiring of mitral cell dendrites2021

    • Author(s)
      Satoshi Fujimoto, Marcus N. Leiwe, Shuhei Aihara, Richi Sakaguchi, Yuko Muroyama, Reiko Kobayakawa, Ko Kobayakawa, Tetsuichiro Saito, Takeshi Imai
    • Organizer
      第44回日本神経科学大会 CJK第1回国際会議
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] BMPR-2 gates activity-dependent stabilization of dendrites during mitral cell remodeling2021

    • Author(s)
      Shuhei Aihara, Satoshi Fujimoto, Richi Sakaguchi, Takeshi Imai
    • Organizer
      第44回日本神経科学大会 CJK第1回国際会議
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 腸内感覚を担う迷走神経のin vivo カルシウムイメージング2021

    • Author(s)
      武島光里, 藤本聡志, 稲垣成矩, 今井猛
    • Organizer
      第72回西日本生理学会
    • Related Report
      2021 Research-status Report
  • [Presentation] Activity-dependent competition within a neuron ensures the discrete wiring of mitral cell dendrites2020

    • Author(s)
      Satoshi Fujimoto, Marcus N. Leiwe, Shuhei Aihara, Richi Sakaguchi, Yuko Muroyama, Reiko Kobayakawa, Ko Kobayakawa, Tetsuichiro Saito, Takeshi Imai
    • Organizer
      CSHL Meeting Molecular Mechanisms of Neuronal Connectivity
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Selective dendrite stabilization is controlled by BMPR2 in developing mitral cells2020

    • Author(s)
      Shuhei Aihara, Satoshi Fujimoto, Richi Sakaguchi, Takeshi Imai
    • Organizer
      CSHL Meeting Molecular Mechanisms of Neuronal Connectivity
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] Activity-dependent competition within a neuron ensures the discrete wiring of mitral cell dendrites2020

    • Author(s)
      Satoshi Fujimoto
    • Organizer
      遺伝研研究会
    • Related Report
      2020 Research-status Report
  • [Presentation] 生後発達期の嗅球における自発神経活動依存的な回路形成2019

    • Author(s)
      藤本聡志
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Spontaneous activity and olfactory circuit development2019

    • Author(s)
      Satoshi Fujimoto, Marcus N. Leiwe, Shuhei Aihara, *Takeshi Imai
    • Organizer
      中国神経科学学会第13回全国技術会議
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 解析方法、解析装置およびプログラム2021

    • Inventor(s)
      今井猛、マーカス ルーウィ、藤本聡志
    • Industrial Property Rights Holder
      今井猛、マーカス ルーウィ、藤本聡志
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-122029
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report

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Published: 2019-04-18   Modified: 2024-01-30  

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