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Neural Circuit Mechanisms for Olfactory Memory and Motivated Behavior

Research Project

Project/Area Number 19K06894
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 46010:Neuroscience-general-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Miyasaka Nobuhiko  国立研究開発法人理化学研究所, 脳神経科学研究センター, 副チームリーダー (70332335)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords匂い / 神経回路 / 脳 / 行動 / ゼブラフィッシュ / 学習 / 記憶 / モチベーション / 連合学習 / 視床
Outline of Research at the Start

嗅覚系は外界の多種多様な匂い分子(化学物質)を受容し、対象物の認知、情動の誘起、内分泌系の変化、さらには記憶の形成や想起を介して「目的行動への意欲」を生み出す神経システムである。これらの行動には、動物種特異的にプログラムされた「生得的な行動」と、過去の経験に依存する「学習依存的行動」が含まれる。本研究では熱帯魚ゼブラフィッシュを用いて、匂い学習の前後で活動するニューロン集団の相互関係、生得的行動と学習依存的行動に関わる神経回路素子のクロストーク、意欲行動とリンクして活性化する視床亜核の構造と機能を解析し、匂いの入力から行動意欲を生み出す神経回路メカニズムに迫る。

Outline of Final Research Achievements

How learning enables odor cues to evoke goal-oriented behavior remains largely unknown. Here I use a simple behavioral paradigm for appetitive olfactory conditioning in adult zebrafish. Repeated pairings of odorant infusion with feeding result in odorant-evoked attraction of zebrafish prior to feeding. Following the probe trial, the number of c-fos-positive cells in a specific subnucleus of the thalamus is significantly greater in paired group than that in unpaired group. Furthermore, c-fos-positive cells increase in number in the thalamic subnucleus upon exposing naive zebrafish to a novel environment. The thalamic c-fos-positive cells in both the experimental groups are a specific population of excitatory neurons that co-express particular neuropeptide and transcription factor genes. These results suggest that activation of the thalamic neurons correlates with a brain state common to motivated behaviors.

Academic Significance and Societal Importance of the Research Achievements

すべての動物の生存や種の維持にとって欠くことのできない摂食、危険回避、繁殖などの行動には、根源的な意志の力、すなわち意欲が重要な役割を果たしている。本研究では、ゼブラフィッシュの「匂い-エサ報酬連合学習系」を用いて、意欲行動にリンクして活性化する腹内側視床を同定した。魚類の腹内側視床は、これまでほとんど解析されていない未知の脳部位であるが、本研究によって遺伝子発現プロフィールの一端が明らかとなった。本研究成果は、脊椎動物の意欲を生み出す基本原理の解明に貢献することが期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (7 results)

All 2022 2021 2020 2019

All Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Presentation] A neural substrate for motivated behavior in zebrafish2022

    • Author(s)
      Miyasaka N, Yoshihara Y
    • Organizer
      Neuro2022
    • Related Report
      2021 Annual Research Report
  • [Presentation] ゼブラフィッシュで探る意欲の神経メカニズム2021

    • Author(s)
      宮坂信彦
    • Organizer
      Chemosensation and Behavior Workshop 2021
    • Related Report
      2020 Research-status Report
  • [Presentation] A neural substrate for motivated behavior following appetitive olfactory learning in zebrafish2020

    • Author(s)
      Miyasaka N, Yoshihara Y
    • Organizer
      第43回日本神経科学大会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Olfaction and motivated behavior in zebrafish2020

    • Author(s)
      Miyasaka N, Yoshihara Y
    • Organizer
      18th International Symposium on Olfaction and Taste (ISOT)
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Neural circuit mechanisms underlying olfactory memory and motivated behavior in zebrafish2019

    • Author(s)
      Miyasaka N, Yoshihara Y
    • Organizer
      Neuro2019
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 嗅覚記憶と意欲行動の神経回路メカニズム2019

    • Author(s)
      宮坂信彦, 吉原良浩
    • Organizer
      日本味と匂学会第53回大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Olfactory memory and motivated behavior in zebrafish2019

    • Author(s)
      Miyasaka N, Yoshihara Y
    • Organizer
      The 48th Naito Conference on Integrated Sensory Sciences - Pain, Itch, Smell and Taste
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2023-01-30  

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