Development of a method for analysis of sialyl glycans and glycopeptides using sialic acid linkage-specific derivatization
| Project/Area Number |
19K07025
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
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| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology |
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Principal Investigator |
Tsumoto Hiroki 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 主任研究員 (00409385)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 質量分析 / 糖鎖 / 糖ペプチド / シアル酸 / 誘導体化 |
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| Outline of Research at the Start |
本研究では、独自に開発したシアル酸結合様式特異的アルキルアミド化法と質量分析法を組み合わせ、生物学的に異なる役割を果たしているシアル酸の結合様式まで区別することができる新規シアリル化糖鎖・糖ペプチド解析法の開発を目的とする。また、確立した分析手法をヒト血漿タンパク質の糖鎖・糖ペプチド解析に応用し、加齢に伴う糖鎖構造変化を明らかにする。
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| Outline of Final Research Achievements |
In this study, a method for analysis of sialyl glycans and glycopeptides using sialic acid linkage-specific derivatization was developed to discriminate between alpha2,3- and alpha2,6-sialic acid.
Semi-supercentenarians (SSCs, older than 105 years old) are a good example of human longevity, therefore physiological and biochemical analyses of SSCs can contribute to the understanding of healthy human aging. Using a combination of the developed method and LC-MS/MS, N-glycomics and N-glycoproteomics of plasma proteins obtained from SSCs and younger control groups were performed. As a result of LC-MS/MS analyses, 1676 N-glycopeptides from 91 proteins were identified and quantified. Among them, 366 N-glycopeptides were altered in SSCs compared to younger control groups. Furthermore, multivariate analysis identified 10 N-glycopeptides which were characteristic of SSCs. Most of the identified N-glycopeptides were derived from alpha1-acid glycoprotein 1.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題により開発された解析法を用いることにより，これまではLC分離や標準品入手が困難で解析不可能であった高分岐かつ高シアリル化糖鎖や糖ペプチドの結合様式の違いを容易に区別できる．それにより，より詳細なグライコミクスやグライコプロテオミクス研究の推進や疾患バイオマーカーの探索研究への貢献が期待できる．
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Report
(4 results)
Research Products
(10 results)
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[Presentation] N-Glycan profiling in plasma proteins of semi-supercentenarians using sialic acid linkage-specific derivatization and negative-ion MALDI-TOF MS2022
Author(s)
Hiroki Tsumoto, Mako Inuzuka, Takashi Nishikaze, Yusaku Hioki, Md. Amir Hossen, Yasumichi Arai, Kazunori Ikebe, Tatsuro Ishizaki, Kei Kamide, Yasuyuki Gondo, Yukie Masui, Nobuyoshi Hirose, Yoshihiro Hayakawa, Yuri Miura, Tamao Endo
Organizer
70th ASMS Conference on Mass Spectrometry and Allied Topics
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Int'l Joint Research
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[Presentation] 血しょうタンパク質糖鎖修飾の健康寿命延伸を目指したバイオマーカーとしての可能性．2020
Author(s)
三浦ゆり, 川上恭司郎, 井出野佑太, 増井幸恵, 稲垣宏樹, 小野口航, 津元裕樹, 梅澤啓太郎, 新井康通, 池邉一典, 石崎達郎, 神出計, 権藤恭之, 遠藤玉夫．
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第71回日本電気泳動学会総会．
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[Presentation] 安定同位体を用いたSALSA法による比較質量分析法の開発．2020
Author(s)
花松久寿, 西風隆司, 津元裕樹, 小川浩司, 横田育子, 森川賢一, 須田剛生, 庄拓也, 中井正人, 三浦信明, 関谷禎規, 岩本慎一, 三浦ゆり, 田中耕一, 坂本直哉, 古川潤一．
Organizer
第39回日本糖質学会年会．
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