Analysis of intestinal microflora and obesity-resistance properties of Suncus murinus

• Project/Area Number: 19K07271
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 48010:Anatomy-related
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: Yi Shuang-Qin 東京都立大学, 人間健康科学研究科, 教授 (70334753)
• Co-Investigator(Kenkyū-buntansha): 佐々木 啓 順天堂大学, スポーツ健康科学部, 准教授 (20384969) ; 任 可 東京都立大学, 人間健康科学研究科, 客員研究員 (60837863)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 腸内細菌叢移植 / 短鎖脂肪酸 / 乳酸菌属 / 16S rRNA / Suncus murinus / 抗生剤投与 / 抗肥満 / 益生菌 / 腸内細菌叢 / 肥満 / 糞の移植 / 次世代DNAシーケエンサー（NGS） / 抗生剤のカクテル / 高脂肪食 / 糞移植 / スンクス / 腸間膜脂肪 / 内臓脂肪蓄積 / 次世代DNAシーケンサー / 腸間膜脂肪蓄積

Outline of Research at the Start

１）メタゲノムを用いたスンクスの腸内細菌叢の解析：スンクスを安楽死させた後，腸管を開き，糞（腸内細菌叢）を採集し，クローンライブラリー法，次世代DNAシーケンサー（NGS）解析法などを用いて，スンクスの腸内細菌叢を解析する。
２）スンクスの腸内細菌代謝パスウェイ経路の解析とトランスクリプトーム解析。
３）スンクスと肥満マウスの間の腸内細菌叢の相互移植を行う。離乳直後のrecipientに抗菌薬Ampicillinを3週間投与した後，donorからの盲腸内容物（糞）を経口的に投与する。移植実施3週間後，recipientとcontrol動物に対して，体脂肪・内臓脂肪や腸内細菌叢の変化などを解析する。

Outline of Final Research Achievements

As a result of analyzing the intestinal microflora of Suncus, the genus Firmicutes and Proteobacteria were abundant, but the genus Bacteroidetes was not detected, and the genus Lactobacillus was present abundantly. It is speculated that these factors may be related to obesity-resistance property of Suncus. In a gut microbiota transplantation experimental study, recipient C57BL mice showed significant changed in gut microbial composition and bacterial diversity. That was, although it could not completely colonize the intestinal tract of mice, it exerted a certain influence on the establishment of the intestinal microflora of mice. In addition, antibiotic treatment of recipient mice had a greater impact on transplant outcome when compared to analysis of results from antibiotic-only control groups.

Academic Significance and Societal Importance of the Research Achievements

本研究は初めて、天然抗肥満実験動物スンクスの腸内細菌叢をFPFマウスに腸内移植実験を行い、移植の条件などおよびその後の解析より、スンクスの抗肥満特性のメカニズムの解析に一歩前進し、ヒト肥満の形成、特に腸内細菌叢と肥満との関連についての解析に大きな貢献ができたと考えられる。

Research Products

• [Journal Article] Fecal microbiota transplantation from Suncus murinus, an obesity-resistant animal, to C57BL/6NCrSIc mice, and the antibiotic effects in the approach (2023)
  • Author(s): Zhang M, Sasaki H, Yang T, Chen J, Li R, Yi C, Li J, He M and Yi SQ
  • Journal Title: Front. Microbiol Volume: 00 Pages: 00-00
  • DOI: 10.3389/fmicb.2023.1138983
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Retrospective Histopathological Study of Pancreatic Fibrosis in Cadaver Samples (2023)
  • Author(s): 8.Juefei Chen, Ting Yang, Shuichi Nakagawa, Rujia Li, Mingshou Zhang, Ke Ren, Yidan Dai, Iwao Sato, Jun Li, Shuang-Qin Yi
  • Journal Title: J Gstro Hepato Volume: V9(15) Pages: 1-7
  • DOI: 10.2139/ssrn.4288092
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells. (2023)
  • Author(s): Yao-Qi Zhou , Tian-Shang Bao, Jia-Xuan Xie, Lin-Li Yao, Si-Te Yu, Qing Li, Pei-Qi Huang, Wan-Zhen Zhou, Yang-Yang Wang, Su-Yuan Chen, Xiao-Qi Wang, Xue-Li Zhang, Shu-Heng Jiang, Shuang-Qin Yi, Zhi-Gang Zhang, Ming-Ze Ma, Li-Peng Hu, Jia Xu, Jun Li.
  • Journal Title: Cell Oncol (Dordr) Volume: 00 Issue: 4 Pages: 00-00
  • DOI: 10.1007/s13402-023-00795-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spatiotemporal quantification of metastatic tumour cell growth and distribution in lymph nodes by whole-mount tissue 3D imaging. (2022)
  • Author(s): Li J, Xu CJ, Tian GA, Li Q, Li DX, Yan F, Zhou YQ, Huang PQ, Xie JX, Wang X, Jiang SH, Wang YH, Song J, Zhang XL, Yi SQ, Hu LP, Xu Q, Li XW, Zhang ZG.
  • Journal Title: Int J Biol Sci Volume: 18(10) Issue: 10 Pages: 3993-4005
  • DOI: 10.7150/ijbs.72552
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Heat shock protein A4L is a potent autoantigen for testicular autoimmunity in mice (2021)
  • Author(s): Nagahori Kenta、Hirai Shuichi、Hatayama Naoyuki、Kuramasu Miyuki、Omotehara Takuya、Kawata Shinichi、Li Zhonglian、Miyaso Hidenobu、Ogawa Yuki、Qu Ning、Terayama Hayato、Hayashi Shogo、Yi Shuang-Qin、Naito Munekazu、Itoh Masahiro
  • Journal Title: Journal of Reproductive Immunology Volume: 145 Pages: 103318-103318
  • DOI: 10.1016/j.jri.2021.103318
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 5-Aminolevulinic acid combined with sodium ferrous citrate (5-ALA/SFC) ameliorated liver injury in a murine acute graft-versus-host disease model by reducing inflammation responses through PGC1-α activation (2020)
  • Author(s): 1.Wang ZD, K, Liu C, Hu X, Que WT, Ito H, Takahashi K, Nakajima M, Tanaka T, Ren K, Guo WZ, Yi SQ, Li XK.
  • Journal Title: Drug Discoveries & Therapeutics Volume: 14 Issue: 6 Pages: 304-312
  • DOI: 10.5582/ddt.2020.03112
  • NAID: 130007974245
  • ISSN: 1881-7831, 1881-784X
  • Year and Date: 2020-12-31
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Anatomy of the coronary arteries in fetal pigs: comparison with human anatomy. (2020)
  • Author(s): Dai Y, Yi K, Shimada K, Ren K, Wang Z, Terayama H, Li Xiao K, Yi SQ.
  • Journal Title: Anat Sci Int. Volume: 95 Issue: 2 Pages: 265-276
  • DOI: 10.1007/s12565-019-00516-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Precise anatomical resection based on structures of nerve and fibrous tissue around the superior mesenteric artery for mesopancreas dissection in pancreaticoduodenectomy for pancreatic cancer. (2020)
  • Author(s): Nagakawa Y, Yi SQ, Takishita C, Sahara Y, Osakabe H, Kiya Y, Yamaguchi H, Miwa Y, Sato I, Tsuchida A.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 000 Issue: 6 Pages: 000-000
  • DOI: 10.1002/jhbp.725
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Morphogenesis of the ventral pancreas anlagen is influenced by the SMA branching pattern. (2020)
  • Author(s): Dai Y, Kurosawa K, Ren K, Miwa Y, Sato I, Liu T, Lu X, Yi SQ.
  • Journal Title: Ann Anat. Volume: 14 Pages: 000-000
  • DOI: 10.1016/j.aanat.2020.151481
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Tamarix chinensis Lour inhibits chronic ethanol-induced liver injury in mice. (2020)
  • Author(s): Wang ZD, Zhang Y, Dai YD, Ren K, Han C, Wang HX, Yi SQ.
  • Journal Title: World J Gastroenterol. Volume: 26 Issue: 12 Pages: 1286-1297
  • DOI: 10.3748/wjg.v26.i12.1286
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Mesopancreas and Pancreatic Head Plexus: Morphological, Developmental, and Clinical Perspectives. (2020)
  • Author(s): Yi SQ, Nagakawa Y, Ren K, Dai YD, Zhang MS, Chen JF, Wang ZD, Miwa Y, Liu T, Lu XM.
  • Journal Title: Surg Radiol Aant Volume: 42 Issue: 12 Pages: 1501-1508
  • DOI: 10.1007/s00276-020-02547-y
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] High Fat Diet Load Study in Natural Obesity-Resistant Animal Model, Suncus murinus. (2020)
  • Author(s): 3.Zhang MS, Dai YD, Sasaki H, Terayama H, Ren K, Wang ZD, Chen JF, Yamamoto Y, Yi SQ.
  • Journal Title: J Veterina Sci Res. Volume: 2 Pages: 19-29
  • DOI: 10.36811/jvsr.2020.110011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Variation of insertion of the pectoralis major in a cadaveric study: a case report. (2020)
  • Author(s): 2.Katsuki S, Terayama H, Tanaka R, Qu N, Nomura H, Kawakami S, Umemoto K, Suyama K, Yi SQ, Suzuki T, Sakabe K.
  • Journal Title: Medicine Volume: 99 Issue: 31 Pages: 000-000
  • DOI: 10.1097/md.0000000000021475
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The distribution of nerves supplying the testis, epididymis and accessory sex glands of Suncus murinus (2019)
  • Author(s): Dai Y, Ren K, Kurosawa K, Terayama H, Miwa Y, Sato I, Yi SQ
  • Journal Title: Anat Sci Int Volume: 94(1) Issue: 1 Pages: 128-135
  • DOI: 10.1007/s12565-018-0459-5
  • Peer Reviewed / Open Access
• [Journal Article] Clinical anatomy of the anterior and posterior hepatic plexuses, including relations with the pancreatic plexus: a cadaver study (2019)
  • Author(s): Ren K, Yi SQ, Dai Y, Kurosawa K, Miwa Y, Sato I
  • Journal Title: Clin Anat Volume: 00 Issue: 5 Pages: 00-00
  • DOI: 10.1002/ca.23470
  • Peer Reviewed
• [Journal Article] Prolonged cold ischemia time in mouse heart transplantation using supercooling preservation (2019)
  • Author(s): 4.Que W, Hu X, Fujino M, Terayama H, Sakabe K, Fukunishi N, Zhu P, Yi SQ, Yamada Y, Zhong L, Li XK
  • Journal Title: Transplantation Volume: 00 Issue: 9 Pages: 00-00
  • DOI: 10.1097/tp.0000000000003089
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 膵炎大全２～膵炎・Up to date～ 臓の発生と構造 (2019)
  • Author(s): 易 勤
  • Journal Title: 胆と膵 Volume: 40 Pages: 1031-1037
• [Presentation] ブタに存在する下腿前面の二関節筋の報告 (2023)
  • Author(s): 夏山裕太郎、張明寿、Yang Ting、時田幸之輔、小島龍平、易勤
  • Organizer: 第128回日本解剖学会総会・全国学術集会
• [Presentation] ラットにおける尾部懸垂方法の検討 (2023)
  • Author(s): 清水知恵、張明寿、根岸徹、夏山裕太郎、植竹駿一、易 勤
  • Organizer: 第128回日本解剖学会総会・全国学術集会
• [Presentation] Pathological study of the follow-up analysis of pancreatic disease before death in a cadaver sample (2023)
  • Author(s): Shuang-Qin Yi, Juefei Chen, Ting Yang, Rujia Li,. Mingshou Zhang, Yutarou Natsuyama, Iwao Sato
  • Organizer: 第128回日本解剖学会総会・全国学術集会
• [Presentation] Effect of gut microbiota transplantation of obesity-resistant animal Suncus murinus on the gut flora of C57BL/NcrSIc mice (2023)
  • Author(s): Mingshou Zhang、Hiraku Sasaki、Ting Yang、Juefei Chen、Rujia Li, Shuang-Qin Yi
  • Organizer: 第128回日本解剖学会総会・全国学術集会
• [Presentation] Retrospective histopathological study of pancreatic fatty degeneration in cadaver samples (2023)
  • Author(s): Ting Yang, Rujia Li, Juefei Chen, Mingshou Zhang, Yutarou Natsuyama, Iwao Sato, Shuang-Qin Yi
  • Organizer: 第128回日本解剖学会総会・全国学術集会
• [Presentation] 胎児ブタを用いた解剖学実習と研究の経験に基づく報告 (2023)
  • Author(s): 夏山 裕太郎、易 勤
  • Organizer: 第128回日本解剖学会総会・全国学術集会
  • Invited
• [Presentation] ブタを用いた膝内側関節包の連続性に関する組織学的研究 (2022)
  • Author(s): 夏山裕太郎、島田和幸、張明寿、Yang Ting、易勤
  • Organizer: 第20回学術集会・総会
• [Presentation] Cadaverにおける膵臓の脂肪変性の病理学研究 (2022)
  • Author(s): Ting Yang、Juefei Chen、Mingshou Zhang、Rujia Li、島田和幸、夏山裕太郎、佐藤 巌、易 勤
  • Organizer: コ・メディカル形態機能学会 第20回学術集会・総会
• [Presentation] 腹側膵と背側膵の血管供給の比較解剖学研究 (2022)
  • Author(s): 易 勤、Mingshou Zhang、Ting Yang、島田和幸、夏山裕太郎、Rujia Li、Juefei Chen、植竹駿一
  • Organizer: コ・メディカル形態機能学会 第20回学術集会・総会
• [Presentation] 関節半月内縁の栄養供給に関わるサブ要素 (2022)
  • Author(s): 夏山裕太郎、島田和幸、張明寿、Yang Ting、易 勤
  • Organizer: 日本解剖学会第110回関東支部学術集会
• [Presentation] Cadaverを用いた生前膵臓の脂肪変性の病理学研究 (2022)
  • Author(s): Ting Yang、Juefei Chen、Rujia Li、Mingshou Zhang、島田和幸、夏山裕太郎、佐藤 巌、易 勤
  • Organizer: 日本解剖学会第110回関東支部学術集会
• [Presentation] Pathological study of the follow-up analysis of pancreatic disease before death in a cadaver sample (2022)
  • Author(s): Shuang-Qin Yi, Juefei Chen, Ting Yang, Yoko Miwa, Mingshou Zhang, Rujia Li, Jun Li, Yuichi Nagakawa
  • Organizer: The 26th Meeting of International Association of Pancreatology (IAP) and The 53rd Annual Meeting of Japan Pancreas Society (JPS)
  • Int'l Joint Research
• [Presentation] Gut microbiota transplantation experimental study in anti-visceral fat accumulation experimental animal Suncus murinus to C57BL/NcrSIc mice (2022)
  • Author(s): Mingshou Zhang、Hiraku Sasaki、Ting Yang、Juefei Chen、Rujia Li, Shuang-Qin Yi
  • Organizer: 第127回日本解剖学会総会・全国学術集会
• [Presentation] Pathological study of the follow-up analysis of pancreatic disease before death in a cadaver sample (2022)
  • Author(s): Juefei Chen, Ting Yang, Yoko Miwa, Mingshou Zhang, Rujia Li, Shuang-Qin Yi
  • Organizer: 第127回日本解剖学会総会・全国学術集会
• [Presentation] 内側側副靭帯と関節包の結合における組織学的検討 (2022)
  • Author(s): 夏山裕太郎、張明寿、楊テイ、島田和幸、易勤
  • Organizer: 第127回日本解剖学会総会・全国学術集会
• [Presentation] Clinical Anatomy of the Anterior and Posterior Hepatic Plexuses. (2021)
  • Author(s): Shuang-Qin Yi, Yoko Miwa, Ke Ren, Yuichi Nagakawa.
  • Organizer: 日本膵臓学会
• [Presentation] Study of age-related change in femur left-right differences in rats. (2021)
  • Author(s): Shunichi Uetake, Toru Negishi, Ming-Shou Zhang, Shuang-Qin Yi.
  • Organizer: 日本解剖学会総会
• [Presentation] The distribution and ramification of the coronary artery in fetus pigs (2019)
  • Author(s): Dai Y, Shimada K, Ren K, Fujiwara M, Kuruzawa K, Wang Z, Yi SQ
  • Organizer: 第124回日本解剖学会総会・全国学術集会
• [Presentation] コ・メディカル向け解剖学国家試験対策e-learningサイトの構築 (2019)
  • Author(s): 黒澤一弘、易 勤
  • Organizer: 第124回日本解剖学会総会・全国学術集会
• [Presentation] Morphogenesis of the ventral pancreas anlagen is related to derivation of the inferior pancreatic artery? (2019)
  • Author(s): Yi SQ, Kurosawa k, Dai Y, Ren K, Miwa Y, Sato I
  • Organizer: 第124回日本解剖学会総会・全国学術集会
• [Presentation] 高齢者膝関節における大腿骨軟骨と半月板の退行性変化について (2019)
  • Author(s): 渡辺 新之助、冨岡 大樹、高橋 雅人、上田 容子、佐藤 巌、易 勤
  • Organizer: 第124回日本解剖学会総会・全国学術集会
• [Presentation] 胎児ブタを用いた膝内側側副靭帯・半月板の血管支配の比較解剖研究 (2019)
  • Author(s): 藤原 基、ニン カ、ダイ イダン、黒澤 一弘、オウ シダン、堀江 俊裕、岡田 昌也、易 勤
  • Organizer: 第124回日本解剖学会総会・全国学術集会
• [Presentation] Morphogenesis of the Ventral Pancreas Anlagen is Influenced by the SMA Branching Pattern (2019)
  • Author(s): Yidan Dai, Kazuhiro Kurosawa, Ke Ren, Yoko Miwa, Iwao Sato, Tao Liu, Xiaoming Lu, Shuang-Qin Yi
  • Organizer: The 50th Anniversary Joint Meeting of American Pancreatic Association (APA) and Japan Pancreas Society (JPS)
  • Int'l Joint Research
• [Presentation] The Mesopancreas and Pancreatic Head Plexus: Morphological, Developmental, and Clinical Perspectives (2019)
  • Author(s): Shuang-Qin Yi, Yi-Dan Dai, Ke Ren, Mingshou Zhang, Yuichi Nagakawa, Yoko Miwa, Iwao Sato, Tao Liu, Xiao-Ming Lu
  • Organizer: The 50th Anniversary Joint Meeting of American Pancreatic Association (APA) and Japan Pancreas Society (JPS)
  • Int'l Joint Research
• [Book] 膵臓病診療ガイドブック (2020)
  • Author(s): 易 勤（分担）
  • Publisher: 診断と治療社（株）