| Project/Area Number |
19K07526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 49040:Parasitology-related
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| Research Institution | Ehime University (2020-2021)
Nagasaki University (2019) |
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Principal Investigator |
Culleton Richard 愛媛大学, プロテオサイエンスセンター, 教授 (10503782)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Malaria / Genetics / Genomics / malaria / P. vinckei / vaccine / LGS / RMP / Plasmodium vinckei / WGS / genetics / crossing / Vaccine / Genetic linkage analysis |
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| Outline of Research at the Start |
In order to design new vaccines for malaria, proteins that play crucial roles in inducing protective host immunity must be identified. I propose to identify targets of host immunity using Linkage Group Selection (LGS) and the experimental rodent malaria parasite system, Plasmodium vinckei. I propose systematically test the ability of combinations of strains of P. vinckei to induce strain specific immunity in mice, then apply LGS to attempt to identify the genes that encode protective antigens.
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| Outline of Final Research Achievements |
We have generated a comprehensive genetic resource for P. vinckei comprising of five reference-quality genomes, one for each of its subspecies, blood-stage RNA sequencing data for five P. vinckei isolates, and genotypes and growth phenotypes for ten isolates. Additionally, we sequenced seven isolates of the RMP species Plasmodium chabaudi and Plasmodium yoelii, thus extending genotypic information for four additional subspecies enabling a re-evaluation of the genotypic diversity and evolutionary history of RMPs. The subspecies from the highland forests of Katanga, P. v. vinckei, has a uniquely smaller genome, a reduced multigene family repertoire and is also amenable to transfection making it an ideal parasite for reverse genetics. We also show that P. vinckei parasites are amenable to genetic crosses.
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| Academic Significance and Societal Importance of the Research Achievements |
Plasmodium vinckei isolates display a large degree of phenotypic and genotypic diversity and could serve as a resource to study parasite virulence and immunogenicity. Amenability to genetic crossing and transfection make them also suitable for classical and functional genetics to study malaria.
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