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Generation mechanisms and functions of small RNA derived from influenza virus

Research Project

Project/Area Number 19K07576
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49060:Virology-related
Research InstitutionNagasaki University (2021-2022)
Kyoto University (2019-2020)

Principal Investigator

Furuse Yuki  長崎大学, 医歯薬学総合研究科(医学系), 教授 (50740940)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsウイルス / RNA / 機能性RNA / ゲノム / 進化 / インフルエンザ / インフルエンザウイルス
Outline of Research at the Start

インフルエンザの病態を理解するためには、インフルエンザウイルスの増殖機構や宿主細胞との相互作用を解明することが重要である。そのためには、ウイルスゲノム上にコードされるタンパク質だけではなくゲノム上の塩基配列に由来する機能性RNAの理解が必須である。最近、インフルエンザウイルスのRNAゲノムに由来する small RNA が発見されたが、その意義や機能はいまだ不明である。
そこで本研究では、①インフルエンザウイルス small RNA がつくられるメカニズム、②インフルエンザウイルス small RNA のウイルス生活環における機能、の2点の解明を試みる。

Outline of Final Research Achievements

The study aimed to reveal the physiological functions of RNA molecules derived from the RNA genome of influenza virus. In this study, we found that the length and expression level of small RNA derived from the RNA genome are determined by the nucleotide sequence in the corresponding region in the genome. We further showed that the small RNA plays a role in the transition between replication and transcription of viral genes. The presence of RNA modifications in viral RNAs was also explored. Finally, genetic mutations under selective sweep in the viral RNAs were identified using a novel informatics algorithm.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、インフルエンザウイルスに由来するRNAの持つさまざまな性質が明らかになった。ウイルスに由来するRNA分子がウイルスの増殖機構や、宿主とウイルスの相互作用に関与していることが示されたことから、これらのRNA分子はインフルエンザの病原性に関わっていると考えられる。そのため本成果をもとにした今後の研究によって、新規治療薬開発のターゲットを見つけられる可能性がある。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (8 results)

All 2022 2021 2020 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] Cartography of SARS‐CoV‐2 variants based on the susceptibility to therapeutic monoclonal antibodies2022

    • Author(s)
      Furuse Yuki
    • Journal Title

      Journal of Medical Virology

      Volume: 95 Issue: 1

    • DOI

      10.1002/jmv.28275

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA Modifications in Genomic RNA of Influenza A Virus and the Relationship between RNA Modifications and Viral Infection2021

    • Author(s)
      Furuse Yuki
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 17 Pages: 9127-9127

    • DOI

      10.3390/ijms22179127

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identifying Potentially Beneficial Genetic Mutations Associated with Monophyletic Selective Sweep and a Proof-of-Concept Study with Viral Genetic Data2021

    • Author(s)
      Furuse Yuki
    • Journal Title

      mSystems

      Volume: 6 Issue: 1

    • DOI

      10.1128/msystems.01151-20

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] RNA modifications on viral genome and viral transcripts of influenza A virus.2022

    • Author(s)
      Furuse Y
    • Organizer
      Options XI for the control of influenza
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Virus Research from Cellular to Global Level2021

    • Author(s)
      古瀬祐気
    • Organizer
      FRIS Annual Meeting 2021 / 1st TI-FRIS International Symposium
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel Algorithm to Identify Beneficial Genetic Mutations in Viral Genome: Ebola, Influenza, and SARS-CoV-22020

    • Author(s)
      古瀬祐気
    • Organizer
      The 15th International Symposium of the Institute Network for Biomedical Sciences 2020
    • Related Report
      2020 Research-status Report
  • [Remarks] YUKI FURUSE Lab

    • URL

      https://furuse-lab.mystrikingly.com/

    • Related Report
      2021 Research-status Report
  • [Remarks] YUKI FURUSE Lab

    • URL

      http://furuse-lab.mystrikingly.com/

    • Related Report
      2019 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

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